Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000300250 |
Resumo: | ABSTRACT Toxoplasmosis is a zoonotic infection caused by the protozoan parasite Toxoplasma gondii. The infection is widely disseminated in the human population and is usually benign or asymptomatic. Systemic T. gondii infection presents risks for pregnant women and AIDS patients. Although rare, T. gondii can cause outbreaks in urban centers. The origin of these outbreaks is not completely understood but probably results from introduction of zoonotic T. gondii strains in the population. During such outbreaks other pathogens which mimic T. gondii acute febrile syndrome may also circulate; therefore, detailed investigation of the outbreak is of extreme importance. In this study we performed viral metagenomics next-generation sequencing (mNGS) in patient samples obtained during T. gondii outbreak in Santa Maria city, South Brazil. Specific bioinformatics pipelines specialized in virus discovery were applied in order to identify co-circulating vial agents. Epstein Barr virus and Parvovirus B19 contigs were assembled and these viruses can cause symptoms similar to toxoplasmosis. In conclusion, our findings show the importance of Metagenomics next generation sequencing (mNGS) use to help characterize the outbreak more completely and in the management of the affected patients. |
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Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south BrazilToxoplasma gondiiViral metagenomicsEpstein-Barr virusHuman Parvovirus B19ABSTRACT Toxoplasmosis is a zoonotic infection caused by the protozoan parasite Toxoplasma gondii. The infection is widely disseminated in the human population and is usually benign or asymptomatic. Systemic T. gondii infection presents risks for pregnant women and AIDS patients. Although rare, T. gondii can cause outbreaks in urban centers. The origin of these outbreaks is not completely understood but probably results from introduction of zoonotic T. gondii strains in the population. During such outbreaks other pathogens which mimic T. gondii acute febrile syndrome may also circulate; therefore, detailed investigation of the outbreak is of extreme importance. In this study we performed viral metagenomics next-generation sequencing (mNGS) in patient samples obtained during T. gondii outbreak in Santa Maria city, South Brazil. Specific bioinformatics pipelines specialized in virus discovery were applied in order to identify co-circulating vial agents. Epstein Barr virus and Parvovirus B19 contigs were assembled and these viruses can cause symptoms similar to toxoplasmosis. In conclusion, our findings show the importance of Metagenomics next generation sequencing (mNGS) use to help characterize the outbreak more completely and in the management of the affected patients.Brazilian Society of Infectious Diseases2020-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000300250Brazilian Journal of Infectious Diseases v.24 n.3 2020reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2020.04.011info:eu-repo/semantics/openAccessBezerra,Rafael dos SantosDiefenbach,Cristiane FraçãoPereira,Dalnei VeigaKashima,SimoneSlavov,Svetoslav Naneveng2020-08-14T00:00:00Zoai:scielo:S1413-86702020000300250Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2020-08-14T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil |
title |
Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil |
spellingShingle |
Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil Bezerra,Rafael dos Santos Toxoplasma gondii Viral metagenomics Epstein-Barr virus Human Parvovirus B19 |
title_short |
Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil |
title_full |
Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil |
title_fullStr |
Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil |
title_full_unstemmed |
Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil |
title_sort |
Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil |
author |
Bezerra,Rafael dos Santos |
author_facet |
Bezerra,Rafael dos Santos Diefenbach,Cristiane Fração Pereira,Dalnei Veiga Kashima,Simone Slavov,Svetoslav Nanev |
author_role |
author |
author2 |
Diefenbach,Cristiane Fração Pereira,Dalnei Veiga Kashima,Simone Slavov,Svetoslav Nanev |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Bezerra,Rafael dos Santos Diefenbach,Cristiane Fração Pereira,Dalnei Veiga Kashima,Simone Slavov,Svetoslav Nanev |
dc.subject.por.fl_str_mv |
Toxoplasma gondii Viral metagenomics Epstein-Barr virus Human Parvovirus B19 |
topic |
Toxoplasma gondii Viral metagenomics Epstein-Barr virus Human Parvovirus B19 |
description |
ABSTRACT Toxoplasmosis is a zoonotic infection caused by the protozoan parasite Toxoplasma gondii. The infection is widely disseminated in the human population and is usually benign or asymptomatic. Systemic T. gondii infection presents risks for pregnant women and AIDS patients. Although rare, T. gondii can cause outbreaks in urban centers. The origin of these outbreaks is not completely understood but probably results from introduction of zoonotic T. gondii strains in the population. During such outbreaks other pathogens which mimic T. gondii acute febrile syndrome may also circulate; therefore, detailed investigation of the outbreak is of extreme importance. In this study we performed viral metagenomics next-generation sequencing (mNGS) in patient samples obtained during T. gondii outbreak in Santa Maria city, South Brazil. Specific bioinformatics pipelines specialized in virus discovery were applied in order to identify co-circulating vial agents. Epstein Barr virus and Parvovirus B19 contigs were assembled and these viruses can cause symptoms similar to toxoplasmosis. In conclusion, our findings show the importance of Metagenomics next generation sequencing (mNGS) use to help characterize the outbreak more completely and in the management of the affected patients. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000300250 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000300250 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjid.2020.04.011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.24 n.3 2020 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209245060923392 |