Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations

Detalhes bibliográficos
Autor(a) principal: Leal,Elida A.
Data de Publicação: 2017
Outros Autores: Moreira,Josimar D., Nunes,Fernanda F., Souza,Larissa R., Martins,Janaina M., Toledo,Vicente P.C., Almeida,Alzira M.P., Guimarães,Tania M.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600620
Resumo: ABSTRACT Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response. Methods: The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40 µg or 20 µg for each preparation. Immunophenotyping was performed by flow cytometry. Results: Animals immunized with the YP total extract did not elicit detectable anti-F1 antibodies (Ab) in the hemaglutination/inhibition (HA/HI) test. Animals immunized with 40 µg or 20 µg of the F1 protein produced anti-F1 Abs, with titres ranging from 1/16 to 1/8132. The average of CD3+-CD4+ and CD3+-CD8+ T cells did not differ significantly between the groups. Neither YP total extract nor F1 protein induced a significant expression of IFN-γ and IL-10 in CD4+ T lymphocytes. In addition, F1 failed to induce IFN-γ expression in CD8+ T cells, unlike the YP total extract. Conclusion: The results showed that F1 protein is not an immunogenic T cell antigen, although the YP total extract (40 µg dose) favoured CD8+ T cell-mediated cellular immunity.
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spelling Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparationsYersinia pestisAntigensMiceImmune responseVaccinesABSTRACT Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response. Methods: The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40 µg or 20 µg for each preparation. Immunophenotyping was performed by flow cytometry. Results: Animals immunized with the YP total extract did not elicit detectable anti-F1 antibodies (Ab) in the hemaglutination/inhibition (HA/HI) test. Animals immunized with 40 µg or 20 µg of the F1 protein produced anti-F1 Abs, with titres ranging from 1/16 to 1/8132. The average of CD3+-CD4+ and CD3+-CD8+ T cells did not differ significantly between the groups. Neither YP total extract nor F1 protein induced a significant expression of IFN-γ and IL-10 in CD4+ T lymphocytes. In addition, F1 failed to induce IFN-γ expression in CD8+ T cells, unlike the YP total extract. Conclusion: The results showed that F1 protein is not an immunogenic T cell antigen, although the YP total extract (40 µg dose) favoured CD8+ T cell-mediated cellular immunity.Brazilian Society of Infectious Diseases2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600620Brazilian Journal of Infectious Diseases v.21 n.6 2017reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2017.09.001info:eu-repo/semantics/openAccessLeal,Elida A.Moreira,Josimar D.Nunes,Fernanda F.Souza,Larissa R.Martins,Janaina M.Toledo,Vicente P.C.Almeida,Alzira M.P.Guimarães,Tania M.P.eng2017-12-11T00:00:00Zoai:scielo:S1413-86702017000600620Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2017-12-11T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
title Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
spellingShingle Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
Leal,Elida A.
Yersinia pestis
Antigens
Mice
Immune response
Vaccines
title_short Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
title_full Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
title_fullStr Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
title_full_unstemmed Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
title_sort Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
author Leal,Elida A.
author_facet Leal,Elida A.
Moreira,Josimar D.
Nunes,Fernanda F.
Souza,Larissa R.
Martins,Janaina M.
Toledo,Vicente P.C.
Almeida,Alzira M.P.
Guimarães,Tania M.P.
author_role author
author2 Moreira,Josimar D.
Nunes,Fernanda F.
Souza,Larissa R.
Martins,Janaina M.
Toledo,Vicente P.C.
Almeida,Alzira M.P.
Guimarães,Tania M.P.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leal,Elida A.
Moreira,Josimar D.
Nunes,Fernanda F.
Souza,Larissa R.
Martins,Janaina M.
Toledo,Vicente P.C.
Almeida,Alzira M.P.
Guimarães,Tania M.P.
dc.subject.por.fl_str_mv Yersinia pestis
Antigens
Mice
Immune response
Vaccines
topic Yersinia pestis
Antigens
Mice
Immune response
Vaccines
description ABSTRACT Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response. Methods: The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40 µg or 20 µg for each preparation. Immunophenotyping was performed by flow cytometry. Results: Animals immunized with the YP total extract did not elicit detectable anti-F1 antibodies (Ab) in the hemaglutination/inhibition (HA/HI) test. Animals immunized with 40 µg or 20 µg of the F1 protein produced anti-F1 Abs, with titres ranging from 1/16 to 1/8132. The average of CD3+-CD4+ and CD3+-CD8+ T cells did not differ significantly between the groups. Neither YP total extract nor F1 protein induced a significant expression of IFN-γ and IL-10 in CD4+ T lymphocytes. In addition, F1 failed to induce IFN-γ expression in CD8+ T cells, unlike the YP total extract. Conclusion: The results showed that F1 protein is not an immunogenic T cell antigen, although the YP total extract (40 µg dose) favoured CD8+ T cell-mediated cellular immunity.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600620
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600620
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjid.2017.09.001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.21 n.6 2017
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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