Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea

Detalhes bibliográficos
Autor(a) principal: Kumari,Aloka
Data de Publicação: 2017
Outros Autores: Baskaran,Ponnusamy, Staden,Johannes Van
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300228
Resumo: Abstract Developing a tissue culture-independent genetic transformation system would be an interesting technique for gene transfer in valuable medicinal and horticultural plants. Efficient gene delivery (Agrobacterium tumefaciens strain LBA 4404: harbouring PBI121 plasmid) was achieved with Km-resistant pollen grains (pollen tube technique) and were found to be GUS-positive for Albuca nelsonii (31.3%) and Tulbaghia violacea (32.6%). The Km-resistance (95.6% for A. nelsonii and 86.7% for T. violacea) and GUS-positive (100% for A. nelsonii and 97.5% for T. violacea) putative transgenic seedlings in vitro were obtained with 200 mg L-1 Km. The in vitro plants were obtained from leaf explants of putative transgenic seedlings and were confirmed to be Km-resistant and GUS-positive (T. violacea, 73.7% and A. nelsonii, 80.5%). The plants were successfully acclimatized in the greenhouse. We describe a tissue culture-independent gene transfer technique with high efficiency clonal transgenic plant production for A. nelsonii and T. violacea. This can also be applied to biotechnological crop improvement of the same species and potentially to other plants.
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spelling Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violaceaAgrobacterium tumefaciensgenetic transformationGUS-expressionin vitro regenerationpollen transformationAbstract Developing a tissue culture-independent genetic transformation system would be an interesting technique for gene transfer in valuable medicinal and horticultural plants. Efficient gene delivery (Agrobacterium tumefaciens strain LBA 4404: harbouring PBI121 plasmid) was achieved with Km-resistant pollen grains (pollen tube technique) and were found to be GUS-positive for Albuca nelsonii (31.3%) and Tulbaghia violacea (32.6%). The Km-resistance (95.6% for A. nelsonii and 86.7% for T. violacea) and GUS-positive (100% for A. nelsonii and 97.5% for T. violacea) putative transgenic seedlings in vitro were obtained with 200 mg L-1 Km. The in vitro plants were obtained from leaf explants of putative transgenic seedlings and were confirmed to be Km-resistant and GUS-positive (T. violacea, 73.7% and A. nelsonii, 80.5%). The plants were successfully acclimatized in the greenhouse. We describe a tissue culture-independent gene transfer technique with high efficiency clonal transgenic plant production for A. nelsonii and T. violacea. This can also be applied to biotechnological crop improvement of the same species and potentially to other plants.Crop Breeding and Applied Biotechnology2017-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300228Crop Breeding and Applied Biotechnology v.17 n.3 2017reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332017v17n3a35info:eu-repo/semantics/openAccessKumari,AlokaBaskaran,PonnusamyStaden,Johannes Vaneng2017-07-21T00:00:00Zoai:scielo:S1984-70332017000300228Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-07-21T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
title Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
spellingShingle Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
Kumari,Aloka
Agrobacterium tumefaciens
genetic transformation
GUS-expression
in vitro regeneration
pollen transformation
title_short Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
title_full Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
title_fullStr Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
title_full_unstemmed Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
title_sort Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
author Kumari,Aloka
author_facet Kumari,Aloka
Baskaran,Ponnusamy
Staden,Johannes Van
author_role author
author2 Baskaran,Ponnusamy
Staden,Johannes Van
author2_role author
author
dc.contributor.author.fl_str_mv Kumari,Aloka
Baskaran,Ponnusamy
Staden,Johannes Van
dc.subject.por.fl_str_mv Agrobacterium tumefaciens
genetic transformation
GUS-expression
in vitro regeneration
pollen transformation
topic Agrobacterium tumefaciens
genetic transformation
GUS-expression
in vitro regeneration
pollen transformation
description Abstract Developing a tissue culture-independent genetic transformation system would be an interesting technique for gene transfer in valuable medicinal and horticultural plants. Efficient gene delivery (Agrobacterium tumefaciens strain LBA 4404: harbouring PBI121 plasmid) was achieved with Km-resistant pollen grains (pollen tube technique) and were found to be GUS-positive for Albuca nelsonii (31.3%) and Tulbaghia violacea (32.6%). The Km-resistance (95.6% for A. nelsonii and 86.7% for T. violacea) and GUS-positive (100% for A. nelsonii and 97.5% for T. violacea) putative transgenic seedlings in vitro were obtained with 200 mg L-1 Km. The in vitro plants were obtained from leaf explants of putative transgenic seedlings and were confirmed to be Km-resistant and GUS-positive (T. violacea, 73.7% and A. nelsonii, 80.5%). The plants were successfully acclimatized in the greenhouse. We describe a tissue culture-independent gene transfer technique with high efficiency clonal transgenic plant production for A. nelsonii and T. violacea. This can also be applied to biotechnological crop improvement of the same species and potentially to other plants.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300228
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300228
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332017v17n3a35
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.17 n.3 2017
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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