Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)

Detalhes bibliográficos
Autor(a) principal: Cid-Contreras,Roberto Carlos
Data de Publicação: 2019
Outros Autores: Mascorro-Gallardo,José Oscar, Valadez-Moctezuma,Ernestina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000300285
Resumo: Abstract Genotypes of the chrysanthemum cultivars Harman and Indianapolis were transformed using Agrobacterium tumefaciens strain LBA4404, which carries the binary plasmid pBIN19::Rd29A::ScTPS1TPS2::nos and pBIN19::35S::ScTPS1TPS2::nos, respectively. ISSR markers were used to discriminate two cultivars from each other, and independent transgenic plants generated in each cultivar. Firstly, the nptII gene and Tnos sequence in the transformed genotypes was confirmed by PCR. For genotyping, 10 ISSR profile markers produced 131 DNA bands. The percentage of polymorphism ranged from 20 to 82.4 %. Primer UBC 872 provided the highest percentage of polymorphic bands (PBP), polymorphic information content (PIC), marker index (MI) and resolving power (Rp). A positive correlation was found between the Rp value of each primer and the number of identified genotypes (r=0.822***). The UPGMA analysis generated two groups with a similarity coefficient of 0.67. The genotype grouping was confirmed by PCoA. The ISSR technique was efficient to discriminate transgenic cultivars from non-transgenic plants.
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spelling Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)Agrobacteriumgenomic differentiationgenotypingISSR markerstransgenic plantsAbstract Genotypes of the chrysanthemum cultivars Harman and Indianapolis were transformed using Agrobacterium tumefaciens strain LBA4404, which carries the binary plasmid pBIN19::Rd29A::ScTPS1TPS2::nos and pBIN19::35S::ScTPS1TPS2::nos, respectively. ISSR markers were used to discriminate two cultivars from each other, and independent transgenic plants generated in each cultivar. Firstly, the nptII gene and Tnos sequence in the transformed genotypes was confirmed by PCR. For genotyping, 10 ISSR profile markers produced 131 DNA bands. The percentage of polymorphism ranged from 20 to 82.4 %. Primer UBC 872 provided the highest percentage of polymorphic bands (PBP), polymorphic information content (PIC), marker index (MI) and resolving power (Rp). A positive correlation was found between the Rp value of each primer and the number of identified genotypes (r=0.822***). The UPGMA analysis generated two groups with a similarity coefficient of 0.67. The genotype grouping was confirmed by PCoA. The ISSR technique was efficient to discriminate transgenic cultivars from non-transgenic plants.Crop Breeding and Applied Biotechnology2019-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000300285Crop Breeding and Applied Biotechnology v.19 n.3 2019reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332019v19n3a40info:eu-repo/semantics/openAccessCid-Contreras,Roberto CarlosMascorro-Gallardo,José OscarValadez-Moctezuma,Ernestinaeng2019-10-29T00:00:00Zoai:scielo:S1984-70332019000300285Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2019-10-29T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
title Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
spellingShingle Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
Cid-Contreras,Roberto Carlos
Agrobacterium
genomic differentiation
genotyping
ISSR markers
transgenic plants
title_short Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
title_full Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
title_fullStr Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
title_full_unstemmed Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
title_sort Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
author Cid-Contreras,Roberto Carlos
author_facet Cid-Contreras,Roberto Carlos
Mascorro-Gallardo,José Oscar
Valadez-Moctezuma,Ernestina
author_role author
author2 Mascorro-Gallardo,José Oscar
Valadez-Moctezuma,Ernestina
author2_role author
author
dc.contributor.author.fl_str_mv Cid-Contreras,Roberto Carlos
Mascorro-Gallardo,José Oscar
Valadez-Moctezuma,Ernestina
dc.subject.por.fl_str_mv Agrobacterium
genomic differentiation
genotyping
ISSR markers
transgenic plants
topic Agrobacterium
genomic differentiation
genotyping
ISSR markers
transgenic plants
description Abstract Genotypes of the chrysanthemum cultivars Harman and Indianapolis were transformed using Agrobacterium tumefaciens strain LBA4404, which carries the binary plasmid pBIN19::Rd29A::ScTPS1TPS2::nos and pBIN19::35S::ScTPS1TPS2::nos, respectively. ISSR markers were used to discriminate two cultivars from each other, and independent transgenic plants generated in each cultivar. Firstly, the nptII gene and Tnos sequence in the transformed genotypes was confirmed by PCR. For genotyping, 10 ISSR profile markers produced 131 DNA bands. The percentage of polymorphism ranged from 20 to 82.4 %. Primer UBC 872 provided the highest percentage of polymorphic bands (PBP), polymorphic information content (PIC), marker index (MI) and resolving power (Rp). A positive correlation was found between the Rp value of each primer and the number of identified genotypes (r=0.822***). The UPGMA analysis generated two groups with a similarity coefficient of 0.67. The genotype grouping was confirmed by PCoA. The ISSR technique was efficient to discriminate transgenic cultivars from non-transgenic plants.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000300285
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000300285
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332019v19n3a40
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.19 n.3 2019
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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