Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense

Detalhes bibliográficos
Autor(a) principal: Moreira,Nádia Fernandes
Data de Publicação: 2017
Outros Autores: Pereira,Telma Nair Santana, Martins,Kellen Coutinho
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200159
Resumo: Abstract The aim of this study was to observe the genetic relationship between C. frutescens (UENF 1636) and C. chinense (UENF 1785) based on the meiotic behavior, on the meiotic index, and on pollen viability of their F1 hybrids. For meiotic analysis and meiotic index, flower buds were collected and fixed for 24 hours in ethanol: acetic acid (3:1), and then transferred to 70% ethanol, and stored in a freezer. Slides preparation for meiosis, meiotic index and pollen viability was carried out according to the laboratory’s protocol. Hybrid meiosis was regular, and 12 pairs of chromosomes were observed in diakinesis, as well as a few anomalies. The MI of 88.16%, as well as the pollen viability of 72.5%, were satisfactory. Given the results, it was confirmed the genetic proximity of these species, which are classified in the Capsicum annuum gene complex.
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spelling Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinensePepperchromosomescytogeneticsAbstract The aim of this study was to observe the genetic relationship between C. frutescens (UENF 1636) and C. chinense (UENF 1785) based on the meiotic behavior, on the meiotic index, and on pollen viability of their F1 hybrids. For meiotic analysis and meiotic index, flower buds were collected and fixed for 24 hours in ethanol: acetic acid (3:1), and then transferred to 70% ethanol, and stored in a freezer. Slides preparation for meiosis, meiotic index and pollen viability was carried out according to the laboratory’s protocol. Hybrid meiosis was regular, and 12 pairs of chromosomes were observed in diakinesis, as well as a few anomalies. The MI of 88.16%, as well as the pollen viability of 72.5%, were satisfactory. Given the results, it was confirmed the genetic proximity of these species, which are classified in the Capsicum annuum gene complex.Crop Breeding and Applied Biotechnology2017-06-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200159Crop Breeding and Applied Biotechnology v.17 n.2 2017reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332017v17n2n23info:eu-repo/semantics/openAccessMoreira,Nádia FernandesPereira,Telma Nair SantanaMartins,Kellen Coutinhoeng2017-03-28T00:00:00Zoai:scielo:S1984-70332017000200159Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-03-28T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
title Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
spellingShingle Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
Moreira,Nádia Fernandes
Pepper
chromosomes
cytogenetics
title_short Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
title_full Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
title_fullStr Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
title_full_unstemmed Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
title_sort Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense
author Moreira,Nádia Fernandes
author_facet Moreira,Nádia Fernandes
Pereira,Telma Nair Santana
Martins,Kellen Coutinho
author_role author
author2 Pereira,Telma Nair Santana
Martins,Kellen Coutinho
author2_role author
author
dc.contributor.author.fl_str_mv Moreira,Nádia Fernandes
Pereira,Telma Nair Santana
Martins,Kellen Coutinho
dc.subject.por.fl_str_mv Pepper
chromosomes
cytogenetics
topic Pepper
chromosomes
cytogenetics
description Abstract The aim of this study was to observe the genetic relationship between C. frutescens (UENF 1636) and C. chinense (UENF 1785) based on the meiotic behavior, on the meiotic index, and on pollen viability of their F1 hybrids. For meiotic analysis and meiotic index, flower buds were collected and fixed for 24 hours in ethanol: acetic acid (3:1), and then transferred to 70% ethanol, and stored in a freezer. Slides preparation for meiosis, meiotic index and pollen viability was carried out according to the laboratory’s protocol. Hybrid meiosis was regular, and 12 pairs of chromosomes were observed in diakinesis, as well as a few anomalies. The MI of 88.16%, as well as the pollen viability of 72.5%, were satisfactory. Given the results, it was confirmed the genetic proximity of these species, which are classified in the Capsicum annuum gene complex.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200159
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200159
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332017v17n2n23
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.17 n.2 2017
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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