Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide

Detalhes bibliográficos
Autor(a) principal: Rutherford,RS
Data de Publicação: 2017
Outros Autores: Maphalala,KZ, Koch,AC, Snyman,SJ, Watt,MP
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200107
Resumo: Abstract Seven imazapyr-tolerant mutant sugarcane plants, previously generated by in vitro mutagenesis, were studied. The imazapyr concentrations required to inhibit their acetolactate synthase (ALS basal activity) (IC50 as μmoles acetoin h-1 mg-1 protein) were 0.77 - 5.36 times greater than that of the N12 ‘parent’. The basal ALS activities of Mut1 and Mut6 were 1.4-fold higher than that of N12. When the mutants were sprayed with Arsenal® GEN 2 (312 and 624 g a.i. imazapyr ha-1), 2 months after field planting, and evaluated 9 months later, live stalk height and number were significantly lowest in Mut2, Mut3 and the control N12. No differences in sucrose, fibre and estimated yield were observed amongst lines in untreated plots. Mutant plants germinated and grew in soil treated with the herbicide (at the lethal dose of 1248 g a.i. ha-1). The Mut lines tested in this study offer improved options for weed control.
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spelling Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicideAcetolactate synthaseethyl methanesulfonateimidazolinonemutation breedingAbstract Seven imazapyr-tolerant mutant sugarcane plants, previously generated by in vitro mutagenesis, were studied. The imazapyr concentrations required to inhibit their acetolactate synthase (ALS basal activity) (IC50 as μmoles acetoin h-1 mg-1 protein) were 0.77 - 5.36 times greater than that of the N12 ‘parent’. The basal ALS activities of Mut1 and Mut6 were 1.4-fold higher than that of N12. When the mutants were sprayed with Arsenal® GEN 2 (312 and 624 g a.i. imazapyr ha-1), 2 months after field planting, and evaluated 9 months later, live stalk height and number were significantly lowest in Mut2, Mut3 and the control N12. No differences in sucrose, fibre and estimated yield were observed amongst lines in untreated plots. Mutant plants germinated and grew in soil treated with the herbicide (at the lethal dose of 1248 g a.i. ha-1). The Mut lines tested in this study offer improved options for weed control.Crop Breeding and Applied Biotechnology2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200107Crop Breeding and Applied Biotechnology v.17 n.2 2017reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332017v17n2a17info:eu-repo/semantics/openAccessRutherford,RSMaphalala,KZKoch,ACSnyman,SJWatt,MPeng2017-03-28T00:00:00Zoai:scielo:S1984-70332017000200107Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-03-28T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
spellingShingle Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
Rutherford,RS
Acetolactate synthase
ethyl methanesulfonate
imidazolinone
mutation breeding
title_short Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_full Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_fullStr Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_full_unstemmed Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_sort Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
author Rutherford,RS
author_facet Rutherford,RS
Maphalala,KZ
Koch,AC
Snyman,SJ
Watt,MP
author_role author
author2 Maphalala,KZ
Koch,AC
Snyman,SJ
Watt,MP
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rutherford,RS
Maphalala,KZ
Koch,AC
Snyman,SJ
Watt,MP
dc.subject.por.fl_str_mv Acetolactate synthase
ethyl methanesulfonate
imidazolinone
mutation breeding
topic Acetolactate synthase
ethyl methanesulfonate
imidazolinone
mutation breeding
description Abstract Seven imazapyr-tolerant mutant sugarcane plants, previously generated by in vitro mutagenesis, were studied. The imazapyr concentrations required to inhibit their acetolactate synthase (ALS basal activity) (IC50 as μmoles acetoin h-1 mg-1 protein) were 0.77 - 5.36 times greater than that of the N12 ‘parent’. The basal ALS activities of Mut1 and Mut6 were 1.4-fold higher than that of N12. When the mutants were sprayed with Arsenal® GEN 2 (312 and 624 g a.i. imazapyr ha-1), 2 months after field planting, and evaluated 9 months later, live stalk height and number were significantly lowest in Mut2, Mut3 and the control N12. No differences in sucrose, fibre and estimated yield were observed amongst lines in untreated plots. Mutant plants germinated and grew in soil treated with the herbicide (at the lethal dose of 1248 g a.i. ha-1). The Mut lines tested in this study offer improved options for weed control.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200107
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200107
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332017v17n2a17
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.17 n.2 2017
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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