Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina

Detalhes bibliográficos
Autor(a) principal: Enrique,Ramón
Data de Publicação: 2021
Outros Autores: Kurth,Daniel, Ibarra-Laclette,Enrique, Noguera,Aldo, Welin,Björn, Castagnaro,Atilio Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000300202
Resumo: Abstract: A prerequisite for commercial release of a transgenic crop variety in Argentina and other countries in the world is the identification and sequence analysis of the transgene(s) insertion site in the plant genome. Southern blot analysis showed a high number of transgene inserts in the glyphosate-resistant transgenic sugarcane line TUC 87-3RG. By using a targeted sequence enrichment approach, coupled with second-generation sequencing (SGS) and bioinformatic analyses, we were able to identify and subsequently analyze all flanking sequences for these transgene insertions in the sugarcane genome. PCR reactions confirmed 18 out of 29 candidate contigs as junction sequences that did not match essential genes in published sugarcane genomes. The methodology outlined here represents an efficient strategy for characterization of flanking sequences corresponding to multiple inserts of a transgene in a large, complex, and not fully sequenced plant genome such as that of sugarcane.
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spelling Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in ArgentinaSugarcanetargeted sequence captureSGSflanking sequencesmultiple insertsAbstract: A prerequisite for commercial release of a transgenic crop variety in Argentina and other countries in the world is the identification and sequence analysis of the transgene(s) insertion site in the plant genome. Southern blot analysis showed a high number of transgene inserts in the glyphosate-resistant transgenic sugarcane line TUC 87-3RG. By using a targeted sequence enrichment approach, coupled with second-generation sequencing (SGS) and bioinformatic analyses, we were able to identify and subsequently analyze all flanking sequences for these transgene insertions in the sugarcane genome. PCR reactions confirmed 18 out of 29 candidate contigs as junction sequences that did not match essential genes in published sugarcane genomes. The methodology outlined here represents an efficient strategy for characterization of flanking sequences corresponding to multiple inserts of a transgene in a large, complex, and not fully sequenced plant genome such as that of sugarcane.Crop Breeding and Applied Biotechnology2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000300202Crop Breeding and Applied Biotechnology v.21 n.3 2021reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332021v21n3a42info:eu-repo/semantics/openAccessEnrique,RamónKurth,DanielIbarra-Laclette,EnriqueNoguera,AldoWelin,BjörnCastagnaro,Atilio Pedroeng2021-10-18T00:00:00Zoai:scielo:S1984-70332021000300202Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2021-10-18T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
title Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
spellingShingle Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
Enrique,Ramón
Sugarcane
targeted sequence capture
SGS
flanking sequences
multiple inserts
title_short Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
title_full Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
title_fullStr Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
title_full_unstemmed Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
title_sort Identification of transgene flanking sequences in a pre-market safety assessed sugarcane in Argentina
author Enrique,Ramón
author_facet Enrique,Ramón
Kurth,Daniel
Ibarra-Laclette,Enrique
Noguera,Aldo
Welin,Björn
Castagnaro,Atilio Pedro
author_role author
author2 Kurth,Daniel
Ibarra-Laclette,Enrique
Noguera,Aldo
Welin,Björn
Castagnaro,Atilio Pedro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Enrique,Ramón
Kurth,Daniel
Ibarra-Laclette,Enrique
Noguera,Aldo
Welin,Björn
Castagnaro,Atilio Pedro
dc.subject.por.fl_str_mv Sugarcane
targeted sequence capture
SGS
flanking sequences
multiple inserts
topic Sugarcane
targeted sequence capture
SGS
flanking sequences
multiple inserts
description Abstract: A prerequisite for commercial release of a transgenic crop variety in Argentina and other countries in the world is the identification and sequence analysis of the transgene(s) insertion site in the plant genome. Southern blot analysis showed a high number of transgene inserts in the glyphosate-resistant transgenic sugarcane line TUC 87-3RG. By using a targeted sequence enrichment approach, coupled with second-generation sequencing (SGS) and bioinformatic analyses, we were able to identify and subsequently analyze all flanking sequences for these transgene insertions in the sugarcane genome. PCR reactions confirmed 18 out of 29 candidate contigs as junction sequences that did not match essential genes in published sugarcane genomes. The methodology outlined here represents an efficient strategy for characterization of flanking sequences corresponding to multiple inserts of a transgene in a large, complex, and not fully sequenced plant genome such as that of sugarcane.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000300202
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000300202
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332021v21n3a42
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.21 n.3 2021
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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