Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis

Detalhes bibliográficos
Autor(a) principal: Chen,Fangyu
Data de Publicação: 2019
Outros Autores: Jiang,Liangrong, Huang,Rongyu, Chen,Xiaolong, Chen,Zhiming, Wang,Houcong, Huang,Yumin, Zheng,Jingsheng
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100110
Resumo: Abstract To identify potential protein markers associated with tillering in rice, a d10 allelic mutant (JHCA) with a high-tillering dwarf phenotype and a wild-type cultivar (GLA4) with normal numbers of tillers were used to produce a hybrid (F1), and 2-DE (two-dimensional electrophoresis) analysis was conducted in seedling leaf sheaths and roots of the rice hybrid and its parents. A total of 61 protein spots representing qualitative or quantitative polymorphisms were detected. Among them, 38 spots were successfully identified as 23 different proteins by MALDI-TOF/MS. The identified proteins were further divided into P/As (presence/absence variations), PSs (position shift variations) and qVs (quantitative variations). Most of the identified polymorphic proteins exhibited tissue-specific expression. Two spot families, identified as a putative vitamin B12-independent methionine synthase and a putative phosphoglycerate mutase were found. The profiles of expression abundance of three iso-spots in each spot family showed a reciprocal relationship between the two parents.
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spelling Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysisProtein polymorphismriceleaf sheathrootproteomicsAbstract To identify potential protein markers associated with tillering in rice, a d10 allelic mutant (JHCA) with a high-tillering dwarf phenotype and a wild-type cultivar (GLA4) with normal numbers of tillers were used to produce a hybrid (F1), and 2-DE (two-dimensional electrophoresis) analysis was conducted in seedling leaf sheaths and roots of the rice hybrid and its parents. A total of 61 protein spots representing qualitative or quantitative polymorphisms were detected. Among them, 38 spots were successfully identified as 23 different proteins by MALDI-TOF/MS. The identified proteins were further divided into P/As (presence/absence variations), PSs (position shift variations) and qVs (quantitative variations). Most of the identified polymorphic proteins exhibited tissue-specific expression. Two spot families, identified as a putative vitamin B12-independent methionine synthase and a putative phosphoglycerate mutase were found. The profiles of expression abundance of three iso-spots in each spot family showed a reciprocal relationship between the two parents.Crop Breeding and Applied Biotechnology2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100110Crop Breeding and Applied Biotechnology v.19 n.1 2019reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332019v19n1a15info:eu-repo/semantics/openAccessChen,FangyuJiang,LiangrongHuang,RongyuChen,XiaolongChen,ZhimingWang,HoucongHuang,YuminZheng,Jingshengeng2019-04-08T00:00:00Zoai:scielo:S1984-70332019000100110Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2019-04-08T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis
title Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis
spellingShingle Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis
Chen,Fangyu
Protein polymorphism
rice
leaf sheath
root
proteomics
title_short Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis
title_full Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis
title_fullStr Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis
title_full_unstemmed Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis
title_sort Visualized protein polymorphisms in leaf sheaths and roots of rice assessed by 2-DE analysis
author Chen,Fangyu
author_facet Chen,Fangyu
Jiang,Liangrong
Huang,Rongyu
Chen,Xiaolong
Chen,Zhiming
Wang,Houcong
Huang,Yumin
Zheng,Jingsheng
author_role author
author2 Jiang,Liangrong
Huang,Rongyu
Chen,Xiaolong
Chen,Zhiming
Wang,Houcong
Huang,Yumin
Zheng,Jingsheng
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chen,Fangyu
Jiang,Liangrong
Huang,Rongyu
Chen,Xiaolong
Chen,Zhiming
Wang,Houcong
Huang,Yumin
Zheng,Jingsheng
dc.subject.por.fl_str_mv Protein polymorphism
rice
leaf sheath
root
proteomics
topic Protein polymorphism
rice
leaf sheath
root
proteomics
description Abstract To identify potential protein markers associated with tillering in rice, a d10 allelic mutant (JHCA) with a high-tillering dwarf phenotype and a wild-type cultivar (GLA4) with normal numbers of tillers were used to produce a hybrid (F1), and 2-DE (two-dimensional electrophoresis) analysis was conducted in seedling leaf sheaths and roots of the rice hybrid and its parents. A total of 61 protein spots representing qualitative or quantitative polymorphisms were detected. Among them, 38 spots were successfully identified as 23 different proteins by MALDI-TOF/MS. The identified proteins were further divided into P/As (presence/absence variations), PSs (position shift variations) and qVs (quantitative variations). Most of the identified polymorphic proteins exhibited tissue-specific expression. Two spot families, identified as a putative vitamin B12-independent methionine synthase and a putative phosphoglycerate mutase were found. The profiles of expression abundance of three iso-spots in each spot family showed a reciprocal relationship between the two parents.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100110
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100110
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332019v19n1a15
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.19 n.1 2019
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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