Identification of haploids and diploids in maize using seedling traits and flow cytometry
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000400203 |
Resumo: | Abstract: The seedling traits is a simple and non-destructive methodology used to identify haploids/diploids in maize. This study aimed at establishing an optimal germination temperature to evaluate this methodology and verify its effectiveness. Haploid and diploid seeds, obtained from crosses with the haploidy inducer TAIL9, were classified using the R1-Navajo marker and placed to germinated in growth chambers maintained at different temperatures. After 96 h, radicle and coleoptile lengths and the number of lateral seminal roots were determined. Cut-off points for these traits were established using Receiver Operating Characteristic curves and a new haploid/diploid classification was performed and compared to the plant phenotype (gold standard) to obtain false discovery rates and false negative rates. The seedling traits methodology successfully differentiated haploids and diploids, proving to be effective in eliminating false positives, selected by the R1-Navajo marker. The temperature of 30 °C was established as the optimal germination temperature for this study. |
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Crop Breeding and Applied Biotechnology |
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Identification of haploids and diploids in maize using seedling traits and flow cytometryDoubled haploidR1-Navajohaploid inducerAbstract: The seedling traits is a simple and non-destructive methodology used to identify haploids/diploids in maize. This study aimed at establishing an optimal germination temperature to evaluate this methodology and verify its effectiveness. Haploid and diploid seeds, obtained from crosses with the haploidy inducer TAIL9, were classified using the R1-Navajo marker and placed to germinated in growth chambers maintained at different temperatures. After 96 h, radicle and coleoptile lengths and the number of lateral seminal roots were determined. Cut-off points for these traits were established using Receiver Operating Characteristic curves and a new haploid/diploid classification was performed and compared to the plant phenotype (gold standard) to obtain false discovery rates and false negative rates. The seedling traits methodology successfully differentiated haploids and diploids, proving to be effective in eliminating false positives, selected by the R1-Navajo marker. The temperature of 30 °C was established as the optimal germination temperature for this study.Crop Breeding and Applied Biotechnology2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000400203Crop Breeding and Applied Biotechnology v.21 n.4 2021reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332021v21n4a54info:eu-repo/semantics/openAccessBaleroni,Alessandra GuedesRé,FabríciaPelozo,AndressaKamphorst,Samuel HenriqueCarneiro,José Walter PedrozaRossi,Robson MarceloScapim,Carlos Albertoeng2022-02-08T00:00:00Zoai:scielo:S1984-70332021000400203Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2022-02-08T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Identification of haploids and diploids in maize using seedling traits and flow cytometry |
title |
Identification of haploids and diploids in maize using seedling traits and flow cytometry |
spellingShingle |
Identification of haploids and diploids in maize using seedling traits and flow cytometry Baleroni,Alessandra Guedes Doubled haploid R1-Navajo haploid inducer |
title_short |
Identification of haploids and diploids in maize using seedling traits and flow cytometry |
title_full |
Identification of haploids and diploids in maize using seedling traits and flow cytometry |
title_fullStr |
Identification of haploids and diploids in maize using seedling traits and flow cytometry |
title_full_unstemmed |
Identification of haploids and diploids in maize using seedling traits and flow cytometry |
title_sort |
Identification of haploids and diploids in maize using seedling traits and flow cytometry |
author |
Baleroni,Alessandra Guedes |
author_facet |
Baleroni,Alessandra Guedes Ré,Fabrícia Pelozo,Andressa Kamphorst,Samuel Henrique Carneiro,José Walter Pedroza Rossi,Robson Marcelo Scapim,Carlos Alberto |
author_role |
author |
author2 |
Ré,Fabrícia Pelozo,Andressa Kamphorst,Samuel Henrique Carneiro,José Walter Pedroza Rossi,Robson Marcelo Scapim,Carlos Alberto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Baleroni,Alessandra Guedes Ré,Fabrícia Pelozo,Andressa Kamphorst,Samuel Henrique Carneiro,José Walter Pedroza Rossi,Robson Marcelo Scapim,Carlos Alberto |
dc.subject.por.fl_str_mv |
Doubled haploid R1-Navajo haploid inducer |
topic |
Doubled haploid R1-Navajo haploid inducer |
description |
Abstract: The seedling traits is a simple and non-destructive methodology used to identify haploids/diploids in maize. This study aimed at establishing an optimal germination temperature to evaluate this methodology and verify its effectiveness. Haploid and diploid seeds, obtained from crosses with the haploidy inducer TAIL9, were classified using the R1-Navajo marker and placed to germinated in growth chambers maintained at different temperatures. After 96 h, radicle and coleoptile lengths and the number of lateral seminal roots were determined. Cut-off points for these traits were established using Receiver Operating Characteristic curves and a new haploid/diploid classification was performed and compared to the plant phenotype (gold standard) to obtain false discovery rates and false negative rates. The seedling traits methodology successfully differentiated haploids and diploids, proving to be effective in eliminating false positives, selected by the R1-Navajo marker. The temperature of 30 °C was established as the optimal germination temperature for this study. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000400203 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000400203 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332021v21n4a54 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.21 n.4 2021 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209188432576512 |