RNAi-mediated resistance to SMV and BYMV in transgenic tobacco

Detalhes bibliográficos
Autor(a) principal: Thu,Lo Thi Mai
Data de Publicação: 2016
Outros Autores: Thuy,Vi Thi Xuan, Duc,Le Hoang, Son,Le Van, Ha,Chu Hoang, Mau,Chu Hoang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300213
Resumo: Abstract Soybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV) are two typical types of viruses that cause mosaic in soybean plants. Multiple viral infections at the same site can lead to 66% to 80% yield reduction. We have aimed to improve SMV and BYMV resistance in Vietnamese soybeans using gene transfer techniques under the mechanism of RNAi. In this study, we present newly generated transgenic tobacco plants carrying RNAi [CPi (SMV-BYMV)] resistance to the two types of viruses; 73.08% of transgenic tobacco lines proved to be fully resistant to SMV and BYMV. In addition, the number of virus copies in transgenic tobacco plants was reduced on average by more than 51% compared to the control plants (wild type). This promising result shows the potential of transerring the CPi (SMV-BYMV) structure in soybean to increase resistance of soybean to SMV and BYMV and advance the aims of antiviral soybean breeding in Vietnam.
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spelling RNAi-mediated resistance to SMV and BYMV in transgenic tobaccoAntiviral soybean breedinggene transferGlycine maxqRT-PCRmosaic virusAbstract Soybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV) are two typical types of viruses that cause mosaic in soybean plants. Multiple viral infections at the same site can lead to 66% to 80% yield reduction. We have aimed to improve SMV and BYMV resistance in Vietnamese soybeans using gene transfer techniques under the mechanism of RNAi. In this study, we present newly generated transgenic tobacco plants carrying RNAi [CPi (SMV-BYMV)] resistance to the two types of viruses; 73.08% of transgenic tobacco lines proved to be fully resistant to SMV and BYMV. In addition, the number of virus copies in transgenic tobacco plants was reduced on average by more than 51% compared to the control plants (wild type). This promising result shows the potential of transerring the CPi (SMV-BYMV) structure in soybean to increase resistance of soybean to SMV and BYMV and advance the aims of antiviral soybean breeding in Vietnam.Crop Breeding and Applied Biotechnology2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300213Crop Breeding and Applied Biotechnology v.16 n.3 2016reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332016v16n3a32info:eu-repo/semantics/openAccessThu,Lo Thi MaiThuy,Vi Thi XuanDuc,Le HoangSon,Le VanHa,Chu HoangMau,Chu Hoangeng2017-05-18T00:00:00Zoai:scielo:S1984-70332016000300213Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-05-18T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
title RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
spellingShingle RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
Thu,Lo Thi Mai
Antiviral soybean breeding
gene transfer
Glycine max
qRT-PCR
mosaic virus
title_short RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
title_full RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
title_fullStr RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
title_full_unstemmed RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
title_sort RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
author Thu,Lo Thi Mai
author_facet Thu,Lo Thi Mai
Thuy,Vi Thi Xuan
Duc,Le Hoang
Son,Le Van
Ha,Chu Hoang
Mau,Chu Hoang
author_role author
author2 Thuy,Vi Thi Xuan
Duc,Le Hoang
Son,Le Van
Ha,Chu Hoang
Mau,Chu Hoang
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Thu,Lo Thi Mai
Thuy,Vi Thi Xuan
Duc,Le Hoang
Son,Le Van
Ha,Chu Hoang
Mau,Chu Hoang
dc.subject.por.fl_str_mv Antiviral soybean breeding
gene transfer
Glycine max
qRT-PCR
mosaic virus
topic Antiviral soybean breeding
gene transfer
Glycine max
qRT-PCR
mosaic virus
description Abstract Soybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV) are two typical types of viruses that cause mosaic in soybean plants. Multiple viral infections at the same site can lead to 66% to 80% yield reduction. We have aimed to improve SMV and BYMV resistance in Vietnamese soybeans using gene transfer techniques under the mechanism of RNAi. In this study, we present newly generated transgenic tobacco plants carrying RNAi [CPi (SMV-BYMV)] resistance to the two types of viruses; 73.08% of transgenic tobacco lines proved to be fully resistant to SMV and BYMV. In addition, the number of virus copies in transgenic tobacco plants was reduced on average by more than 51% compared to the control plants (wild type). This promising result shows the potential of transerring the CPi (SMV-BYMV) structure in soybean to increase resistance of soybean to SMV and BYMV and advance the aims of antiviral soybean breeding in Vietnam.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300213
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300213
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332016v16n3a32
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.16 n.3 2016
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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