ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya

Detalhes bibliográficos
Autor(a) principal: Costa,Fabiane Rabelo da
Data de Publicação: 2011
Outros Autores: Pereira,Telma Nair Santana, Gabriel,Ana Paula Candido, Pereira,Messias Gonzaga
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000400009
Resumo: ISSR markers are polymorphic and their results easily reproducible. They are therefore intensely used in phylogenetic studies and sex differentiation of some economically interesting plant species. The objectives of this study were to analyze the genetic diversity in Caricaceae using ISSR markers, to identify a specific ISSR band that could distinguish female from hermaphrodite papaya genotypes and to verify whether this marker could be used for early sex differentiation. The ISSR-PCR was performed with nine primers and they could distinguish all species. It was observed that Jacaratia spinosa was closer to Vasconcellea than to Carica. The species C. papaya was only distantly related to both genera. A 500 bp ISSR marker was found in 25 % of the papaya genotypes studied. Specifically in these cases this marker could be used for early sex differentiation in papaya.
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spelling ISSR markers for genetic relationships in Caricaceae and sex differentiation in papayagenetic diversityearly molecular sexingISSR markers are polymorphic and their results easily reproducible. They are therefore intensely used in phylogenetic studies and sex differentiation of some economically interesting plant species. The objectives of this study were to analyze the genetic diversity in Caricaceae using ISSR markers, to identify a specific ISSR band that could distinguish female from hermaphrodite papaya genotypes and to verify whether this marker could be used for early sex differentiation. The ISSR-PCR was performed with nine primers and they could distinguish all species. It was observed that Jacaratia spinosa was closer to Vasconcellea than to Carica. The species C. papaya was only distantly related to both genera. A 500 bp ISSR marker was found in 25 % of the papaya genotypes studied. Specifically in these cases this marker could be used for early sex differentiation in papaya.Crop Breeding and Applied Biotechnology2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000400009Crop Breeding and Applied Biotechnology v.11 n.4 2011reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/S1984-70332011000400009info:eu-repo/semantics/openAccessCosta,Fabiane Rabelo daPereira,Telma Nair SantanaGabriel,Ana Paula CandidoPereira,Messias Gonzagaeng2012-01-06T00:00:00Zoai:scielo:S1984-70332011000400009Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2012-01-06T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
title ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
spellingShingle ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
Costa,Fabiane Rabelo da
genetic diversity
early molecular sexing
title_short ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
title_full ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
title_fullStr ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
title_full_unstemmed ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
title_sort ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
author Costa,Fabiane Rabelo da
author_facet Costa,Fabiane Rabelo da
Pereira,Telma Nair Santana
Gabriel,Ana Paula Candido
Pereira,Messias Gonzaga
author_role author
author2 Pereira,Telma Nair Santana
Gabriel,Ana Paula Candido
Pereira,Messias Gonzaga
author2_role author
author
author
dc.contributor.author.fl_str_mv Costa,Fabiane Rabelo da
Pereira,Telma Nair Santana
Gabriel,Ana Paula Candido
Pereira,Messias Gonzaga
dc.subject.por.fl_str_mv genetic diversity
early molecular sexing
topic genetic diversity
early molecular sexing
description ISSR markers are polymorphic and their results easily reproducible. They are therefore intensely used in phylogenetic studies and sex differentiation of some economically interesting plant species. The objectives of this study were to analyze the genetic diversity in Caricaceae using ISSR markers, to identify a specific ISSR band that could distinguish female from hermaphrodite papaya genotypes and to verify whether this marker could be used for early sex differentiation. The ISSR-PCR was performed with nine primers and they could distinguish all species. It was observed that Jacaratia spinosa was closer to Vasconcellea than to Carica. The species C. papaya was only distantly related to both genera. A 500 bp ISSR marker was found in 25 % of the papaya genotypes studied. Specifically in these cases this marker could be used for early sex differentiation in papaya.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000400009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000400009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-70332011000400009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.11 n.4 2011
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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