Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000400201 |
Resumo: | Abstract Palm trees are the third most important botanical family for humans because of their potential use in oils, drugs, cosmetics, food, and feed. Despite their importance, little information on their genetics and molecular variations exists, and a better understanding could contribute to breeding programs. This study aimed to determine the amount, distribution, and organization of plastid simple sequence repeats (cpSSRs) and their potential use in breeding in 52 species belonging to the order Arecales. Plastid genomes were analyzed to identify cpSSRs according to their nature, position, and presence in genic or intergenic regions. Primer pairs were validated in silico for amplification and polymorphisms in these SSRs and their dissimilarities were evaluated. The results showed a high frequency of mononucleotide repeats in the intergenic regions. Approximately 76 primer pairs were generated and are suggested for further studies. The dissimilarity analysis of cpSSRs showed that mono- and trinucleotides were highly abundant in plastid SSRs. |
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Crop Breeding and Applied Biotechnology |
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Variation in palm tree plastidial simple sequence repeats, characterization, and potential useIn silicochloroplast DNAmarkersprimersAbstract Palm trees are the third most important botanical family for humans because of their potential use in oils, drugs, cosmetics, food, and feed. Despite their importance, little information on their genetics and molecular variations exists, and a better understanding could contribute to breeding programs. This study aimed to determine the amount, distribution, and organization of plastid simple sequence repeats (cpSSRs) and their potential use in breeding in 52 species belonging to the order Arecales. Plastid genomes were analyzed to identify cpSSRs according to their nature, position, and presence in genic or intergenic regions. Primer pairs were validated in silico for amplification and polymorphisms in these SSRs and their dissimilarities were evaluated. The results showed a high frequency of mononucleotide repeats in the intergenic regions. Approximately 76 primer pairs were generated and are suggested for further studies. The dissimilarity analysis of cpSSRs showed that mono- and trinucleotides were highly abundant in plastid SSRs.Crop Breeding and Applied Biotechnology2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000400201Crop Breeding and Applied Biotechnology v.22 n.4 2022reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332022v22n4a36info:eu-repo/semantics/openAccessSilveira,TatieliFreitas,Karine Elise Janner deSantos,Railson Schreinert dosOliveira,Antonio Costa deBarbieri,Rosa Líaeng2022-12-15T00:00:00Zoai:scielo:S1984-70332022000400201Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2022-12-15T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Variation in palm tree plastidial simple sequence repeats, characterization, and potential use |
title |
Variation in palm tree plastidial simple sequence repeats, characterization, and potential use |
spellingShingle |
Variation in palm tree plastidial simple sequence repeats, characterization, and potential use Silveira,Tatieli In silico chloroplast DNA markers primers |
title_short |
Variation in palm tree plastidial simple sequence repeats, characterization, and potential use |
title_full |
Variation in palm tree plastidial simple sequence repeats, characterization, and potential use |
title_fullStr |
Variation in palm tree plastidial simple sequence repeats, characterization, and potential use |
title_full_unstemmed |
Variation in palm tree plastidial simple sequence repeats, characterization, and potential use |
title_sort |
Variation in palm tree plastidial simple sequence repeats, characterization, and potential use |
author |
Silveira,Tatieli |
author_facet |
Silveira,Tatieli Freitas,Karine Elise Janner de Santos,Railson Schreinert dos Oliveira,Antonio Costa de Barbieri,Rosa Lía |
author_role |
author |
author2 |
Freitas,Karine Elise Janner de Santos,Railson Schreinert dos Oliveira,Antonio Costa de Barbieri,Rosa Lía |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silveira,Tatieli Freitas,Karine Elise Janner de Santos,Railson Schreinert dos Oliveira,Antonio Costa de Barbieri,Rosa Lía |
dc.subject.por.fl_str_mv |
In silico chloroplast DNA markers primers |
topic |
In silico chloroplast DNA markers primers |
description |
Abstract Palm trees are the third most important botanical family for humans because of their potential use in oils, drugs, cosmetics, food, and feed. Despite their importance, little information on their genetics and molecular variations exists, and a better understanding could contribute to breeding programs. This study aimed to determine the amount, distribution, and organization of plastid simple sequence repeats (cpSSRs) and their potential use in breeding in 52 species belonging to the order Arecales. Plastid genomes were analyzed to identify cpSSRs according to their nature, position, and presence in genic or intergenic regions. Primer pairs were validated in silico for amplification and polymorphisms in these SSRs and their dissimilarities were evaluated. The results showed a high frequency of mononucleotide repeats in the intergenic regions. Approximately 76 primer pairs were generated and are suggested for further studies. The dissimilarity analysis of cpSSRs showed that mono- and trinucleotides were highly abundant in plastid SSRs. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000400201 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000400201 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332022v22n4a36 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.22 n.4 2022 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209188576231424 |