Variation in palm tree plastidial simple sequence repeats, characterization, and potential use

Detalhes bibliográficos
Autor(a) principal: Silveira,Tatieli
Data de Publicação: 2022
Outros Autores: Freitas,Karine Elise Janner de, Santos,Railson Schreinert dos, Oliveira,Antonio Costa de, Barbieri,Rosa Lía
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000400201
Resumo: Abstract Palm trees are the third most important botanical family for humans because of their potential use in oils, drugs, cosmetics, food, and feed. Despite their importance, little information on their genetics and molecular variations exists, and a better understanding could contribute to breeding programs. This study aimed to determine the amount, distribution, and organization of plastid simple sequence repeats (cpSSRs) and their potential use in breeding in 52 species belonging to the order Arecales. Plastid genomes were analyzed to identify cpSSRs according to their nature, position, and presence in genic or intergenic regions. Primer pairs were validated in silico for amplification and polymorphisms in these SSRs and their dissimilarities were evaluated. The results showed a high frequency of mononucleotide repeats in the intergenic regions. Approximately 76 primer pairs were generated and are suggested for further studies. The dissimilarity analysis of cpSSRs showed that mono- and trinucleotides were highly abundant in plastid SSRs.
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spelling Variation in palm tree plastidial simple sequence repeats, characterization, and potential useIn silicochloroplast DNAmarkersprimersAbstract Palm trees are the third most important botanical family for humans because of their potential use in oils, drugs, cosmetics, food, and feed. Despite their importance, little information on their genetics and molecular variations exists, and a better understanding could contribute to breeding programs. This study aimed to determine the amount, distribution, and organization of plastid simple sequence repeats (cpSSRs) and their potential use in breeding in 52 species belonging to the order Arecales. Plastid genomes were analyzed to identify cpSSRs according to their nature, position, and presence in genic or intergenic regions. Primer pairs were validated in silico for amplification and polymorphisms in these SSRs and their dissimilarities were evaluated. The results showed a high frequency of mononucleotide repeats in the intergenic regions. Approximately 76 primer pairs were generated and are suggested for further studies. The dissimilarity analysis of cpSSRs showed that mono- and trinucleotides were highly abundant in plastid SSRs.Crop Breeding and Applied Biotechnology2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000400201Crop Breeding and Applied Biotechnology v.22 n.4 2022reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332022v22n4a36info:eu-repo/semantics/openAccessSilveira,TatieliFreitas,Karine Elise Janner deSantos,Railson Schreinert dosOliveira,Antonio Costa deBarbieri,Rosa Líaeng2022-12-15T00:00:00Zoai:scielo:S1984-70332022000400201Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2022-12-15T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
title Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
spellingShingle Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
Silveira,Tatieli
In silico
chloroplast DNA
markers
primers
title_short Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
title_full Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
title_fullStr Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
title_full_unstemmed Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
title_sort Variation in palm tree plastidial simple sequence repeats, characterization, and potential use
author Silveira,Tatieli
author_facet Silveira,Tatieli
Freitas,Karine Elise Janner de
Santos,Railson Schreinert dos
Oliveira,Antonio Costa de
Barbieri,Rosa Lía
author_role author
author2 Freitas,Karine Elise Janner de
Santos,Railson Schreinert dos
Oliveira,Antonio Costa de
Barbieri,Rosa Lía
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silveira,Tatieli
Freitas,Karine Elise Janner de
Santos,Railson Schreinert dos
Oliveira,Antonio Costa de
Barbieri,Rosa Lía
dc.subject.por.fl_str_mv In silico
chloroplast DNA
markers
primers
topic In silico
chloroplast DNA
markers
primers
description Abstract Palm trees are the third most important botanical family for humans because of their potential use in oils, drugs, cosmetics, food, and feed. Despite their importance, little information on their genetics and molecular variations exists, and a better understanding could contribute to breeding programs. This study aimed to determine the amount, distribution, and organization of plastid simple sequence repeats (cpSSRs) and their potential use in breeding in 52 species belonging to the order Arecales. Plastid genomes were analyzed to identify cpSSRs according to their nature, position, and presence in genic or intergenic regions. Primer pairs were validated in silico for amplification and polymorphisms in these SSRs and their dissimilarities were evaluated. The results showed a high frequency of mononucleotide repeats in the intergenic regions. Approximately 76 primer pairs were generated and are suggested for further studies. The dissimilarity analysis of cpSSRs showed that mono- and trinucleotides were highly abundant in plastid SSRs.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000400201
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000400201
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332022v22n4a36
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.22 n.4 2022
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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