Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean

Detalhes bibliográficos
Autor(a) principal: Dubiela,Carla
Data de Publicação: 2019
Outros Autores: Montecelli,Tatiane Dalla Nora, Lazzari,Fabiane, Souto,Eliezer Rodrigues de, Schuster,Ivan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100102
Resumo: Abstract This work aimed to develop and validate individual SNP molecular markers previously identified in a genetic association study of resistance to Meloidogyne incognita in soybean using a microarray panel. The markers identified in the array were converted in single TaqMan® markers. The single markers were used to create an SNP genotyping protocol and establish a marker-assisted selection (MAS) routine associated with resistance to M. incognita in soybean. Out of the eight TaqMan® assays tested, three were validated for use in MAS. The MAS protocol developed in this study uses sequential selection. Initially, molecular markers are used to identify susceptible plants; subsequently, the phenotypic evaluation of plants expressing resistance genotype for the markers is carried out, resulting in the accurate identification of resistant plants. The accuracy of this approach for MAS sequential for M. incognita varied from 94 to 96%.
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spelling Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybeanMASSNP markerstaqMan® assaysoybeannematodeend-point PCRAbstract This work aimed to develop and validate individual SNP molecular markers previously identified in a genetic association study of resistance to Meloidogyne incognita in soybean using a microarray panel. The markers identified in the array were converted in single TaqMan® markers. The single markers were used to create an SNP genotyping protocol and establish a marker-assisted selection (MAS) routine associated with resistance to M. incognita in soybean. Out of the eight TaqMan® assays tested, three were validated for use in MAS. The MAS protocol developed in this study uses sequential selection. Initially, molecular markers are used to identify susceptible plants; subsequently, the phenotypic evaluation of plants expressing resistance genotype for the markers is carried out, resulting in the accurate identification of resistant plants. The accuracy of this approach for MAS sequential for M. incognita varied from 94 to 96%.Crop Breeding and Applied Biotechnology2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100102Crop Breeding and Applied Biotechnology v.19 n.1 2019reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332019v19n1a14info:eu-repo/semantics/openAccessDubiela,CarlaMontecelli,Tatiane Dalla NoraLazzari,FabianeSouto,Eliezer Rodrigues deSchuster,Ivaneng2019-04-08T00:00:00Zoai:scielo:S1984-70332019000100102Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2019-04-08T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean
title Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean
spellingShingle Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean
Dubiela,Carla
MAS
SNP markers
taqMan® assay
soybean
nematode
end-point PCR
title_short Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean
title_full Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean
title_fullStr Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean
title_full_unstemmed Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean
title_sort Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean
author Dubiela,Carla
author_facet Dubiela,Carla
Montecelli,Tatiane Dalla Nora
Lazzari,Fabiane
Souto,Eliezer Rodrigues de
Schuster,Ivan
author_role author
author2 Montecelli,Tatiane Dalla Nora
Lazzari,Fabiane
Souto,Eliezer Rodrigues de
Schuster,Ivan
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Dubiela,Carla
Montecelli,Tatiane Dalla Nora
Lazzari,Fabiane
Souto,Eliezer Rodrigues de
Schuster,Ivan
dc.subject.por.fl_str_mv MAS
SNP markers
taqMan® assay
soybean
nematode
end-point PCR
topic MAS
SNP markers
taqMan® assay
soybean
nematode
end-point PCR
description Abstract This work aimed to develop and validate individual SNP molecular markers previously identified in a genetic association study of resistance to Meloidogyne incognita in soybean using a microarray panel. The markers identified in the array were converted in single TaqMan® markers. The single markers were used to create an SNP genotyping protocol and establish a marker-assisted selection (MAS) routine associated with resistance to M. incognita in soybean. Out of the eight TaqMan® assays tested, three were validated for use in MAS. The MAS protocol developed in this study uses sequential selection. Initially, molecular markers are used to identify susceptible plants; subsequently, the phenotypic evaluation of plants expressing resistance genotype for the markers is carried out, resulting in the accurate identification of resistant plants. The accuracy of this approach for MAS sequential for M. incognita varied from 94 to 96%.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100102
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000100102
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332019v19n1a14
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.19 n.1 2019
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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