Evaluation of SRAP markers for mapping of Pisum sativum L.

Detalhes bibliográficos
Autor(a) principal: Guindon,María Fernanda
Data de Publicação: 2016
Outros Autores: Martin,Eugenia, Zayas,Aldana, Cointry,Enrique, Cravero,Vanina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300182
Resumo: Abstract Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 were generated. A total of 25 SRAP primer combinations were evaluated in 45 F2 plants and both parental lines, generating 208 polymorphic bands/markers. The markers were analyzed by the chi-square goodness-of-fit test to check the expected Mendelian segregation ratio. The resulting linkage map consists of 112 genetic markers distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers. The linkage map developed in this study indicates that the SRAP marker system could be applied to mapping studies of pea.
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spelling Evaluation of SRAP markers for mapping of Pisum sativum L.Peaplant breedinglinkage mapF2 populationmolecular markersAbstract Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 were generated. A total of 25 SRAP primer combinations were evaluated in 45 F2 plants and both parental lines, generating 208 polymorphic bands/markers. The markers were analyzed by the chi-square goodness-of-fit test to check the expected Mendelian segregation ratio. The resulting linkage map consists of 112 genetic markers distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers. The linkage map developed in this study indicates that the SRAP marker system could be applied to mapping studies of pea.Crop Breeding and Applied Biotechnology2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300182Crop Breeding and Applied Biotechnology v.16 n.3 2016reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332016v16n3a28info:eu-repo/semantics/openAccessGuindon,María FernandaMartin,EugeniaZayas,AldanaCointry,EnriqueCravero,Vaninaeng2017-05-18T00:00:00Zoai:scielo:S1984-70332016000300182Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-05-18T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Evaluation of SRAP markers for mapping of Pisum sativum L.
title Evaluation of SRAP markers for mapping of Pisum sativum L.
spellingShingle Evaluation of SRAP markers for mapping of Pisum sativum L.
Guindon,María Fernanda
Pea
plant breeding
linkage map
F2 population
molecular markers
title_short Evaluation of SRAP markers for mapping of Pisum sativum L.
title_full Evaluation of SRAP markers for mapping of Pisum sativum L.
title_fullStr Evaluation of SRAP markers for mapping of Pisum sativum L.
title_full_unstemmed Evaluation of SRAP markers for mapping of Pisum sativum L.
title_sort Evaluation of SRAP markers for mapping of Pisum sativum L.
author Guindon,María Fernanda
author_facet Guindon,María Fernanda
Martin,Eugenia
Zayas,Aldana
Cointry,Enrique
Cravero,Vanina
author_role author
author2 Martin,Eugenia
Zayas,Aldana
Cointry,Enrique
Cravero,Vanina
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Guindon,María Fernanda
Martin,Eugenia
Zayas,Aldana
Cointry,Enrique
Cravero,Vanina
dc.subject.por.fl_str_mv Pea
plant breeding
linkage map
F2 population
molecular markers
topic Pea
plant breeding
linkage map
F2 population
molecular markers
description Abstract Linkage maps have become important tools for genetic studies. With the aim of evaluating the SRAP (sequence-related amplified polymorphism) technique for linkage mapping in Pisum sativum L., a F2 mapping population derived from an initial cross between cvs. DDR11 and Zav25 were generated. A total of 25 SRAP primer combinations were evaluated in 45 F2 plants and both parental lines, generating 208 polymorphic bands/markers. The markers were analyzed by the chi-square goodness-of-fit test to check the expected Mendelian segregation ratio. The resulting linkage map consists of 112 genetic markers distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers. The linkage map developed in this study indicates that the SRAP marker system could be applied to mapping studies of pea.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300182
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300182
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332016v16n3a28
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.16 n.3 2016
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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