Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100208 |
Resumo: | Abstract The potato tuber moth (Phthorimaea operculella Zeller) (PTM) is a destructive potato pest. Bacillus thuringiensis cry genes encode crystal proteins that have toxic characteristics against PTM. The aim of the present study was to develop a PTM resistant potato cv. Agria using the synthetic Bt cry1Ab gene. The transformation vector was constructed by inserting the cry1Ab gene into the pBI121 binary expression vector. The Agrobacterium tumefaciens strain AGL1 harboring the pBI35SCry1Ab construct was used for potato transformation. The results indicated that out of 97 putative transformed lines, 30 lines were PCR positive. Southern blot analysis revealed that one to three copies of transgenes were inserted into transgenic lines. The immunoassay test revealed the expression of the Cry protein. Furthermore, the results of bioassays confirmed the high mortality level of neonate larvae. The results of this study suggested that the transgenic lines have significant potential in pest control. |
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Crop Breeding and Applied Biotechnology |
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Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larvaBacillus thuringiensiscry proteinspotato tuber moth (PTM)Solanum tuberosumtransgenic plantAbstract The potato tuber moth (Phthorimaea operculella Zeller) (PTM) is a destructive potato pest. Bacillus thuringiensis cry genes encode crystal proteins that have toxic characteristics against PTM. The aim of the present study was to develop a PTM resistant potato cv. Agria using the synthetic Bt cry1Ab gene. The transformation vector was constructed by inserting the cry1Ab gene into the pBI121 binary expression vector. The Agrobacterium tumefaciens strain AGL1 harboring the pBI35SCry1Ab construct was used for potato transformation. The results indicated that out of 97 putative transformed lines, 30 lines were PCR positive. Southern blot analysis revealed that one to three copies of transgenes were inserted into transgenic lines. The immunoassay test revealed the expression of the Cry protein. Furthermore, the results of bioassays confirmed the high mortality level of neonate larvae. The results of this study suggested that the transgenic lines have significant potential in pest control.Crop Breeding and Applied Biotechnology2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100208Crop Breeding and Applied Biotechnology v.21 n.1 2021reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332021v21n1a9info:eu-repo/semantics/openAccessSalehian,HamedRahnama,HassanDezhsetan,SaraBabaei,Saeideng2021-05-19T00:00:00Zoai:scielo:S1984-70332021000100208Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2021-05-19T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva |
title |
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva |
spellingShingle |
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva Salehian,Hamed Bacillus thuringiensis cry proteins potato tuber moth (PTM) Solanum tuberosum transgenic plant |
title_short |
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva |
title_full |
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva |
title_fullStr |
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva |
title_full_unstemmed |
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva |
title_sort |
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva |
author |
Salehian,Hamed |
author_facet |
Salehian,Hamed Rahnama,Hassan Dezhsetan,Sara Babaei,Saeid |
author_role |
author |
author2 |
Rahnama,Hassan Dezhsetan,Sara Babaei,Saeid |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Salehian,Hamed Rahnama,Hassan Dezhsetan,Sara Babaei,Saeid |
dc.subject.por.fl_str_mv |
Bacillus thuringiensis cry proteins potato tuber moth (PTM) Solanum tuberosum transgenic plant |
topic |
Bacillus thuringiensis cry proteins potato tuber moth (PTM) Solanum tuberosum transgenic plant |
description |
Abstract The potato tuber moth (Phthorimaea operculella Zeller) (PTM) is a destructive potato pest. Bacillus thuringiensis cry genes encode crystal proteins that have toxic characteristics against PTM. The aim of the present study was to develop a PTM resistant potato cv. Agria using the synthetic Bt cry1Ab gene. The transformation vector was constructed by inserting the cry1Ab gene into the pBI121 binary expression vector. The Agrobacterium tumefaciens strain AGL1 harboring the pBI35SCry1Ab construct was used for potato transformation. The results indicated that out of 97 putative transformed lines, 30 lines were PCR positive. Southern blot analysis revealed that one to three copies of transgenes were inserted into transgenic lines. The immunoassay test revealed the expression of the Cry protein. Furthermore, the results of bioassays confirmed the high mortality level of neonate larvae. The results of this study suggested that the transgenic lines have significant potential in pest control. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100208 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100208 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332021v21n1a9 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.21 n.1 2021 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209188391682048 |