Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100210 |
Resumo: | Abstract Interspecific crosses between the more than 520 Passiflora species may or may not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm species identity and to estimate the genetic distances among 48 Passiflora accessions of a germplasm bank. Twenty species were used for crosses within and between distinct Passiflora subgenera. The phylogenetic resolution based on ITS data was superior to that of any single chloroplast marker, recommending it as a DNA barcode for this genus. However, the tree topology based on Bayesian phylogenetic analysis using the combination of the four chloroplast markers possessed greater support for subclades within the subgenus Passiflora, which contains more species of interest for breeders. We could not identify a clear cutoff value of pairwise Kimura two-parameter (K2P) distances to predict success or failure of crosses. Rather, the clustering of species pairs together within the same or closely related phylogenetic subclades predicted the probability of wide cross compatibility more reliably. |
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Crop Breeding and Applied Biotechnology |
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Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruitPassionfruitphylogeneticsDNA barcodinghybridsidentificationAbstract Interspecific crosses between the more than 520 Passiflora species may or may not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm species identity and to estimate the genetic distances among 48 Passiflora accessions of a germplasm bank. Twenty species were used for crosses within and between distinct Passiflora subgenera. The phylogenetic resolution based on ITS data was superior to that of any single chloroplast marker, recommending it as a DNA barcode for this genus. However, the tree topology based on Bayesian phylogenetic analysis using the combination of the four chloroplast markers possessed greater support for subclades within the subgenus Passiflora, which contains more species of interest for breeders. We could not identify a clear cutoff value of pairwise Kimura two-parameter (K2P) distances to predict success or failure of crosses. Rather, the clustering of species pairs together within the same or closely related phylogenetic subclades predicted the probability of wide cross compatibility more reliably.Crop Breeding and Applied Biotechnology2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100210Crop Breeding and Applied Biotechnology v.21 n.1 2021reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332021v21n1a12info:eu-repo/semantics/openAccessInglis,Peter W.Bellon,GracieleJunqueira,Nilton T. V.Faleiro,Fábio G.Ferreira,Márcio E.eng2021-05-19T00:00:00Zoai:scielo:S1984-70332021000100210Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2021-05-19T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit |
title |
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit |
spellingShingle |
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit Inglis,Peter W. Passionfruit phylogenetics DNA barcoding hybrids identification |
title_short |
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit |
title_full |
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit |
title_fullStr |
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit |
title_full_unstemmed |
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit |
title_sort |
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit |
author |
Inglis,Peter W. |
author_facet |
Inglis,Peter W. Bellon,Graciele Junqueira,Nilton T. V. Faleiro,Fábio G. Ferreira,Márcio E. |
author_role |
author |
author2 |
Bellon,Graciele Junqueira,Nilton T. V. Faleiro,Fábio G. Ferreira,Márcio E. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Inglis,Peter W. Bellon,Graciele Junqueira,Nilton T. V. Faleiro,Fábio G. Ferreira,Márcio E. |
dc.subject.por.fl_str_mv |
Passionfruit phylogenetics DNA barcoding hybrids identification |
topic |
Passionfruit phylogenetics DNA barcoding hybrids identification |
description |
Abstract Interspecific crosses between the more than 520 Passiflora species may or may not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm species identity and to estimate the genetic distances among 48 Passiflora accessions of a germplasm bank. Twenty species were used for crosses within and between distinct Passiflora subgenera. The phylogenetic resolution based on ITS data was superior to that of any single chloroplast marker, recommending it as a DNA barcode for this genus. However, the tree topology based on Bayesian phylogenetic analysis using the combination of the four chloroplast markers possessed greater support for subclades within the subgenus Passiflora, which contains more species of interest for breeders. We could not identify a clear cutoff value of pairwise Kimura two-parameter (K2P) distances to predict success or failure of crosses. Rather, the clustering of species pairs together within the same or closely related phylogenetic subclades predicted the probability of wide cross compatibility more reliably. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100210 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100210 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332021v21n1a12 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.21 n.1 2021 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209188394827776 |