In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200176 |
Resumo: | Abstract We report the in vitro induction of polyploids from two cassava cultivars by colchicine treatment. Shoot nodal segments, collected from in vitro cultivated plants of Porquinho and Vassourinha cultivars, were used as explants. Explants were treated in liquid media without and with colchicine (0.05, 0.10 or 0.15%), at 90 rpm, in the dark, for 48 or 96 h. Normal-like and putative-polyploid phenotypes were recovered from in vitro culture, for both cultivars. All plants from the putative-polyploid group were confirmed as tetraploids by flow cytometry and root tip chromosome counting, whereas no association was found between ploidy level and phenotype in normal-like plants. Mixoploids were also recovered from both cultivars. Vassourinha was more responsive to colchicine treatment than Porquinho. Tetraploid plants have fewer, but bigger, stomata guard cells than those in diploids. Colchicine at 0.10% for 96 hours induced a high number of tetraploids in both cultivars. |
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Crop Breeding and Applied Biotechnology |
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In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivarsChimerachromosome doublingcolchicineManihot esculentapolyploidyAbstract We report the in vitro induction of polyploids from two cassava cultivars by colchicine treatment. Shoot nodal segments, collected from in vitro cultivated plants of Porquinho and Vassourinha cultivars, were used as explants. Explants were treated in liquid media without and with colchicine (0.05, 0.10 or 0.15%), at 90 rpm, in the dark, for 48 or 96 h. Normal-like and putative-polyploid phenotypes were recovered from in vitro culture, for both cultivars. All plants from the putative-polyploid group were confirmed as tetraploids by flow cytometry and root tip chromosome counting, whereas no association was found between ploidy level and phenotype in normal-like plants. Mixoploids were also recovered from both cultivars. Vassourinha was more responsive to colchicine treatment than Porquinho. Tetraploid plants have fewer, but bigger, stomata guard cells than those in diploids. Colchicine at 0.10% for 96 hours induced a high number of tetraploids in both cultivars.Crop Breeding and Applied Biotechnology2018-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200176Crop Breeding and Applied Biotechnology v.18 n.2 2018reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332018v18n2a25info:eu-repo/semantics/openAccessMondin,MateusSilva,Paulo Artur Konzen Xavier de Mello eLatado,Rodrigo RochaMourão Filho,Francisco de Assis Alveseng2018-04-25T00:00:00Zoai:scielo:S1984-70332018000200176Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2018-04-25T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars |
title |
In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars |
spellingShingle |
In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars Mondin,Mateus Chimera chromosome doubling colchicine Manihot esculenta polyploidy |
title_short |
In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars |
title_full |
In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars |
title_fullStr |
In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars |
title_full_unstemmed |
In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars |
title_sort |
In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars |
author |
Mondin,Mateus |
author_facet |
Mondin,Mateus Silva,Paulo Artur Konzen Xavier de Mello e Latado,Rodrigo Rocha Mourão Filho,Francisco de Assis Alves |
author_role |
author |
author2 |
Silva,Paulo Artur Konzen Xavier de Mello e Latado,Rodrigo Rocha Mourão Filho,Francisco de Assis Alves |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mondin,Mateus Silva,Paulo Artur Konzen Xavier de Mello e Latado,Rodrigo Rocha Mourão Filho,Francisco de Assis Alves |
dc.subject.por.fl_str_mv |
Chimera chromosome doubling colchicine Manihot esculenta polyploidy |
topic |
Chimera chromosome doubling colchicine Manihot esculenta polyploidy |
description |
Abstract We report the in vitro induction of polyploids from two cassava cultivars by colchicine treatment. Shoot nodal segments, collected from in vitro cultivated plants of Porquinho and Vassourinha cultivars, were used as explants. Explants were treated in liquid media without and with colchicine (0.05, 0.10 or 0.15%), at 90 rpm, in the dark, for 48 or 96 h. Normal-like and putative-polyploid phenotypes were recovered from in vitro culture, for both cultivars. All plants from the putative-polyploid group were confirmed as tetraploids by flow cytometry and root tip chromosome counting, whereas no association was found between ploidy level and phenotype in normal-like plants. Mixoploids were also recovered from both cultivars. Vassourinha was more responsive to colchicine treatment than Porquinho. Tetraploid plants have fewer, but bigger, stomata guard cells than those in diploids. Colchicine at 0.10% for 96 hours induced a high number of tetraploids in both cultivars. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200176 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200176 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332018v18n2a25 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.18 n.2 2018 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209187566452736 |