Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Colégio Brasileiro de Cirurgiões |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912014000300181 |
Resumo: | OBJECTIVE: To standardize a technique for ventilating rat fetuses with Congenital Diaphragmatic Hernia (CDH) using a volume-controlled ventilator.METHODS: Pregnant rats were divided into the following groups: a) control (C); b) exposed to nitrofen with CDH (CDH); and c) exposed to nitrofen without CDH (N-). Fetuses of the three groups were randomly divided into the subgroups ventilated (V) and non-ventilated (N-V). Fetuses were collected on day 21.5 of gestation, weighed and ventilated for 30 minutes using a volume-controlled ventilator. Then the lungs were collected for histological study. We evaluated: body weight (BW), total lung weight (TLW), left lung weight (LLW), ratios TLW / BW and LLW / BW, morphological histology of the airways and causes of failures of ventilation.RESULTS: BW, TLW, LLW, TLW / BW and LLW / BW were higher in C compared with N- (p <0.05) and CDH (p <0.05), but no differences were found between the subgroups V and N-V (p> 0.05). The morphology of the pulmonary airways showed hypoplasia in groups N- and CDH, with no difference between V and N-V (p <0.05). The C and N- groups could be successfully ventilated using a tidal volume of 75 ìl, but the failure of ventilation in the CDH group decreased only when ventilated with 50 ìl.CONCLUSION: Volume ventilation is possible in rats with CDH for a short period and does not alter fetal or lung morphology. |
id |
CBC-1_1ada81eb8baa4ecb02e74841eaf665f8 |
---|---|
oai_identifier_str |
oai:scielo:S0100-69912014000300181 |
network_acronym_str |
CBC-1 |
network_name_str |
Revista do Colégio Brasileiro de Cirurgiões |
repository_id_str |
|
spelling |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic herniaHernia, diaphragmatic/congenitalVentilationModels, animalRatsNitrophenolsOBJECTIVE: To standardize a technique for ventilating rat fetuses with Congenital Diaphragmatic Hernia (CDH) using a volume-controlled ventilator.METHODS: Pregnant rats were divided into the following groups: a) control (C); b) exposed to nitrofen with CDH (CDH); and c) exposed to nitrofen without CDH (N-). Fetuses of the three groups were randomly divided into the subgroups ventilated (V) and non-ventilated (N-V). Fetuses were collected on day 21.5 of gestation, weighed and ventilated for 30 minutes using a volume-controlled ventilator. Then the lungs were collected for histological study. We evaluated: body weight (BW), total lung weight (TLW), left lung weight (LLW), ratios TLW / BW and LLW / BW, morphological histology of the airways and causes of failures of ventilation.RESULTS: BW, TLW, LLW, TLW / BW and LLW / BW were higher in C compared with N- (p <0.05) and CDH (p <0.05), but no differences were found between the subgroups V and N-V (p> 0.05). The morphology of the pulmonary airways showed hypoplasia in groups N- and CDH, with no difference between V and N-V (p <0.05). The C and N- groups could be successfully ventilated using a tidal volume of 75 ìl, but the failure of ventilation in the CDH group decreased only when ventilated with 50 ìl.CONCLUSION: Volume ventilation is possible in rats with CDH for a short period and does not alter fetal or lung morphology.Colégio Brasileiro de Cirurgiões2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912014000300181Revista do Colégio Brasileiro de Cirurgiões v.41 n.3 2014reponame:Revista do Colégio Brasileiro de Cirurgiõesinstname:Colégio Brasileiro de Cirurgiões (CBC)instacron:CBC10.1590/S0100-69912014000300008info:eu-repo/semantics/openAccessGallindo,Rodrigo MeloGonçalves,Frances Lilian LanhellasFigueira,Rebeca LopesSimões,Ana Leda BertonciniSbragia,Lourençoeng2015-09-25T00:00:00Zoai:scielo:S0100-69912014000300181Revistahttp://www.scielo.br/rcbcONGhttps://old.scielo.br/oai/scielo-oai.php||revistacbc@cbc.org.br1809-45460100-6991opendoar:2015-09-25T00:00Revista do Colégio Brasileiro de Cirurgiões - Colégio Brasileiro de Cirurgiões (CBC)false |
dc.title.none.fl_str_mv |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia |
title |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia |
spellingShingle |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia Gallindo,Rodrigo Melo Hernia, diaphragmatic/congenital Ventilation Models, animal Rats Nitrophenols |
title_short |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia |
title_full |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia |
title_fullStr |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia |
title_full_unstemmed |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia |
title_sort |
Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia |
author |
Gallindo,Rodrigo Melo |
author_facet |
Gallindo,Rodrigo Melo Gonçalves,Frances Lilian Lanhellas Figueira,Rebeca Lopes Simões,Ana Leda Bertoncini Sbragia,Lourenço |
author_role |
author |
author2 |
Gonçalves,Frances Lilian Lanhellas Figueira,Rebeca Lopes Simões,Ana Leda Bertoncini Sbragia,Lourenço |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gallindo,Rodrigo Melo Gonçalves,Frances Lilian Lanhellas Figueira,Rebeca Lopes Simões,Ana Leda Bertoncini Sbragia,Lourenço |
dc.subject.por.fl_str_mv |
Hernia, diaphragmatic/congenital Ventilation Models, animal Rats Nitrophenols |
topic |
Hernia, diaphragmatic/congenital Ventilation Models, animal Rats Nitrophenols |
description |
OBJECTIVE: To standardize a technique for ventilating rat fetuses with Congenital Diaphragmatic Hernia (CDH) using a volume-controlled ventilator.METHODS: Pregnant rats were divided into the following groups: a) control (C); b) exposed to nitrofen with CDH (CDH); and c) exposed to nitrofen without CDH (N-). Fetuses of the three groups were randomly divided into the subgroups ventilated (V) and non-ventilated (N-V). Fetuses were collected on day 21.5 of gestation, weighed and ventilated for 30 minutes using a volume-controlled ventilator. Then the lungs were collected for histological study. We evaluated: body weight (BW), total lung weight (TLW), left lung weight (LLW), ratios TLW / BW and LLW / BW, morphological histology of the airways and causes of failures of ventilation.RESULTS: BW, TLW, LLW, TLW / BW and LLW / BW were higher in C compared with N- (p <0.05) and CDH (p <0.05), but no differences were found between the subgroups V and N-V (p> 0.05). The morphology of the pulmonary airways showed hypoplasia in groups N- and CDH, with no difference between V and N-V (p <0.05). The C and N- groups could be successfully ventilated using a tidal volume of 75 ìl, but the failure of ventilation in the CDH group decreased only when ventilated with 50 ìl.CONCLUSION: Volume ventilation is possible in rats with CDH for a short period and does not alter fetal or lung morphology. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912014000300181 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912014000300181 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-69912014000300008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Cirurgiões |
publisher.none.fl_str_mv |
Colégio Brasileiro de Cirurgiões |
dc.source.none.fl_str_mv |
Revista do Colégio Brasileiro de Cirurgiões v.41 n.3 2014 reponame:Revista do Colégio Brasileiro de Cirurgiões instname:Colégio Brasileiro de Cirurgiões (CBC) instacron:CBC |
instname_str |
Colégio Brasileiro de Cirurgiões (CBC) |
instacron_str |
CBC |
institution |
CBC |
reponame_str |
Revista do Colégio Brasileiro de Cirurgiões |
collection |
Revista do Colégio Brasileiro de Cirurgiões |
repository.name.fl_str_mv |
Revista do Colégio Brasileiro de Cirurgiões - Colégio Brasileiro de Cirurgiões (CBC) |
repository.mail.fl_str_mv |
||revistacbc@cbc.org.br |
_version_ |
1754209212686139392 |