Testing intravitreal toxicity of rapamycin in rabbit eyes

Detalhes bibliográficos
Autor(a) principal: Manzano,Roberta Pereira de Almeida
Data de Publicação: 2009
Outros Autores: Peyman,Gholam A., Khan,Palwasha, Kivilcim,Muhamet, Chevez-Barrios,Patricia, Takahashi,Walter
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos brasileiros de oftalmologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492009000100004
Resumo: PURPOSE: To evaluate retinal toxicity of varying doses of rapamycin when injected intravitreally in rabbits. Rapamycin is a potent immunosuppressive agent with significant antitumor and antiangiogenic properties, clinically approved for prevention of organ transplant rejection. METHODS: Twelve New Zealand albino rabbits were divided into four groups. Four different doses of rapamycin were prepared in 0.1 ml: 20 µg, 50 µg, 200 µg, and 1000 µg. Each concentration was injected in one eye of three rabbits, and 0.1 ml volume of sterile BSS was injected into the contralateral eye of the three rabbits. Slit-lamp and fundoscopic examinations were performed and the animals were observed for 2 weeks for signs of infection, inflammation, and toxicity. A baseline ERG was performed before drug treatment and at day 14, after which the rabbits were euthanized. Histology of the enucleated eyes was studied to look for retinal toxicity. RESULTS: ERG results showed some decrease in scotopic response; however this was not dose related. ERG results were normal at 20 µg. Histological results showed no retinal toxicity in all groups. CONCLUSION: Although ERG changes were identified at dosages between 50-1000 µg, the histology of all groups up to 1000 µg did not show any discernable abnormalities.
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spelling Testing intravitreal toxicity of rapamycin in rabbit eyesChoroidal neovascularizationSirolimus/toxicityUveitisVascular endothelial growth factor receptor-1RabbitsPURPOSE: To evaluate retinal toxicity of varying doses of rapamycin when injected intravitreally in rabbits. Rapamycin is a potent immunosuppressive agent with significant antitumor and antiangiogenic properties, clinically approved for prevention of organ transplant rejection. METHODS: Twelve New Zealand albino rabbits were divided into four groups. Four different doses of rapamycin were prepared in 0.1 ml: 20 µg, 50 µg, 200 µg, and 1000 µg. Each concentration was injected in one eye of three rabbits, and 0.1 ml volume of sterile BSS was injected into the contralateral eye of the three rabbits. Slit-lamp and fundoscopic examinations were performed and the animals were observed for 2 weeks for signs of infection, inflammation, and toxicity. A baseline ERG was performed before drug treatment and at day 14, after which the rabbits were euthanized. Histology of the enucleated eyes was studied to look for retinal toxicity. RESULTS: ERG results showed some decrease in scotopic response; however this was not dose related. ERG results were normal at 20 µg. Histological results showed no retinal toxicity in all groups. CONCLUSION: Although ERG changes were identified at dosages between 50-1000 µg, the histology of all groups up to 1000 µg did not show any discernable abnormalities.Conselho Brasileiro de Oftalmologia2009-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492009000100004Arquivos Brasileiros de Oftalmologia v.72 n.1 2009reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.1590/S0004-27492009000100004info:eu-repo/semantics/openAccessManzano,Roberta Pereira de AlmeidaPeyman,Gholam A.Khan,PalwashaKivilcim,MuhametChevez-Barrios,PatriciaTakahashi,Waltereng2009-03-30T00:00:00Zoai:scielo:S0004-27492009000100004Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2009-03-30T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false
dc.title.none.fl_str_mv Testing intravitreal toxicity of rapamycin in rabbit eyes
title Testing intravitreal toxicity of rapamycin in rabbit eyes
spellingShingle Testing intravitreal toxicity of rapamycin in rabbit eyes
Manzano,Roberta Pereira de Almeida
Choroidal neovascularization
Sirolimus/toxicity
Uveitis
Vascular endothelial growth factor receptor-1
Rabbits
title_short Testing intravitreal toxicity of rapamycin in rabbit eyes
title_full Testing intravitreal toxicity of rapamycin in rabbit eyes
title_fullStr Testing intravitreal toxicity of rapamycin in rabbit eyes
title_full_unstemmed Testing intravitreal toxicity of rapamycin in rabbit eyes
title_sort Testing intravitreal toxicity of rapamycin in rabbit eyes
author Manzano,Roberta Pereira de Almeida
author_facet Manzano,Roberta Pereira de Almeida
Peyman,Gholam A.
Khan,Palwasha
Kivilcim,Muhamet
Chevez-Barrios,Patricia
Takahashi,Walter
author_role author
author2 Peyman,Gholam A.
Khan,Palwasha
Kivilcim,Muhamet
Chevez-Barrios,Patricia
Takahashi,Walter
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Manzano,Roberta Pereira de Almeida
Peyman,Gholam A.
Khan,Palwasha
Kivilcim,Muhamet
Chevez-Barrios,Patricia
Takahashi,Walter
dc.subject.por.fl_str_mv Choroidal neovascularization
Sirolimus/toxicity
Uveitis
Vascular endothelial growth factor receptor-1
Rabbits
topic Choroidal neovascularization
Sirolimus/toxicity
Uveitis
Vascular endothelial growth factor receptor-1
Rabbits
description PURPOSE: To evaluate retinal toxicity of varying doses of rapamycin when injected intravitreally in rabbits. Rapamycin is a potent immunosuppressive agent with significant antitumor and antiangiogenic properties, clinically approved for prevention of organ transplant rejection. METHODS: Twelve New Zealand albino rabbits were divided into four groups. Four different doses of rapamycin were prepared in 0.1 ml: 20 µg, 50 µg, 200 µg, and 1000 µg. Each concentration was injected in one eye of three rabbits, and 0.1 ml volume of sterile BSS was injected into the contralateral eye of the three rabbits. Slit-lamp and fundoscopic examinations were performed and the animals were observed for 2 weeks for signs of infection, inflammation, and toxicity. A baseline ERG was performed before drug treatment and at day 14, after which the rabbits were euthanized. Histology of the enucleated eyes was studied to look for retinal toxicity. RESULTS: ERG results showed some decrease in scotopic response; however this was not dose related. ERG results were normal at 20 µg. Histological results showed no retinal toxicity in all groups. CONCLUSION: Although ERG changes were identified at dosages between 50-1000 µg, the histology of all groups up to 1000 µg did not show any discernable abnormalities.
publishDate 2009
dc.date.none.fl_str_mv 2009-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492009000100004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492009000100004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0004-27492009000100004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
dc.source.none.fl_str_mv Arquivos Brasileiros de Oftalmologia v.72 n.1 2009
reponame:Arquivos brasileiros de oftalmologia (Online)
instname:Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO
instname_str Conselho Brasileiro de Oftalmologia (CBO)
instacron_str CBO
institution CBO
reponame_str Arquivos brasileiros de oftalmologia (Online)
collection Arquivos brasileiros de oftalmologia (Online)
repository.name.fl_str_mv Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)
repository.mail.fl_str_mv aboonline@cbo.com.br||abo@cbo.com.br
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