Testing intravitreal toxicity of rapamycin in rabbit eyes
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos brasileiros de oftalmologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492009000100004 |
Resumo: | PURPOSE: To evaluate retinal toxicity of varying doses of rapamycin when injected intravitreally in rabbits. Rapamycin is a potent immunosuppressive agent with significant antitumor and antiangiogenic properties, clinically approved for prevention of organ transplant rejection. METHODS: Twelve New Zealand albino rabbits were divided into four groups. Four different doses of rapamycin were prepared in 0.1 ml: 20 µg, 50 µg, 200 µg, and 1000 µg. Each concentration was injected in one eye of three rabbits, and 0.1 ml volume of sterile BSS was injected into the contralateral eye of the three rabbits. Slit-lamp and fundoscopic examinations were performed and the animals were observed for 2 weeks for signs of infection, inflammation, and toxicity. A baseline ERG was performed before drug treatment and at day 14, after which the rabbits were euthanized. Histology of the enucleated eyes was studied to look for retinal toxicity. RESULTS: ERG results showed some decrease in scotopic response; however this was not dose related. ERG results were normal at 20 µg. Histological results showed no retinal toxicity in all groups. CONCLUSION: Although ERG changes were identified at dosages between 50-1000 µg, the histology of all groups up to 1000 µg did not show any discernable abnormalities. |
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Arquivos brasileiros de oftalmologia (Online) |
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Testing intravitreal toxicity of rapamycin in rabbit eyesChoroidal neovascularizationSirolimus/toxicityUveitisVascular endothelial growth factor receptor-1RabbitsPURPOSE: To evaluate retinal toxicity of varying doses of rapamycin when injected intravitreally in rabbits. Rapamycin is a potent immunosuppressive agent with significant antitumor and antiangiogenic properties, clinically approved for prevention of organ transplant rejection. METHODS: Twelve New Zealand albino rabbits were divided into four groups. Four different doses of rapamycin were prepared in 0.1 ml: 20 µg, 50 µg, 200 µg, and 1000 µg. Each concentration was injected in one eye of three rabbits, and 0.1 ml volume of sterile BSS was injected into the contralateral eye of the three rabbits. Slit-lamp and fundoscopic examinations were performed and the animals were observed for 2 weeks for signs of infection, inflammation, and toxicity. A baseline ERG was performed before drug treatment and at day 14, after which the rabbits were euthanized. Histology of the enucleated eyes was studied to look for retinal toxicity. RESULTS: ERG results showed some decrease in scotopic response; however this was not dose related. ERG results were normal at 20 µg. Histological results showed no retinal toxicity in all groups. CONCLUSION: Although ERG changes were identified at dosages between 50-1000 µg, the histology of all groups up to 1000 µg did not show any discernable abnormalities.Conselho Brasileiro de Oftalmologia2009-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492009000100004Arquivos Brasileiros de Oftalmologia v.72 n.1 2009reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.1590/S0004-27492009000100004info:eu-repo/semantics/openAccessManzano,Roberta Pereira de AlmeidaPeyman,Gholam A.Khan,PalwashaKivilcim,MuhametChevez-Barrios,PatriciaTakahashi,Waltereng2009-03-30T00:00:00Zoai:scielo:S0004-27492009000100004Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2009-03-30T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false |
dc.title.none.fl_str_mv |
Testing intravitreal toxicity of rapamycin in rabbit eyes |
title |
Testing intravitreal toxicity of rapamycin in rabbit eyes |
spellingShingle |
Testing intravitreal toxicity of rapamycin in rabbit eyes Manzano,Roberta Pereira de Almeida Choroidal neovascularization Sirolimus/toxicity Uveitis Vascular endothelial growth factor receptor-1 Rabbits |
title_short |
Testing intravitreal toxicity of rapamycin in rabbit eyes |
title_full |
Testing intravitreal toxicity of rapamycin in rabbit eyes |
title_fullStr |
Testing intravitreal toxicity of rapamycin in rabbit eyes |
title_full_unstemmed |
Testing intravitreal toxicity of rapamycin in rabbit eyes |
title_sort |
Testing intravitreal toxicity of rapamycin in rabbit eyes |
author |
Manzano,Roberta Pereira de Almeida |
author_facet |
Manzano,Roberta Pereira de Almeida Peyman,Gholam A. Khan,Palwasha Kivilcim,Muhamet Chevez-Barrios,Patricia Takahashi,Walter |
author_role |
author |
author2 |
Peyman,Gholam A. Khan,Palwasha Kivilcim,Muhamet Chevez-Barrios,Patricia Takahashi,Walter |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Manzano,Roberta Pereira de Almeida Peyman,Gholam A. Khan,Palwasha Kivilcim,Muhamet Chevez-Barrios,Patricia Takahashi,Walter |
dc.subject.por.fl_str_mv |
Choroidal neovascularization Sirolimus/toxicity Uveitis Vascular endothelial growth factor receptor-1 Rabbits |
topic |
Choroidal neovascularization Sirolimus/toxicity Uveitis Vascular endothelial growth factor receptor-1 Rabbits |
description |
PURPOSE: To evaluate retinal toxicity of varying doses of rapamycin when injected intravitreally in rabbits. Rapamycin is a potent immunosuppressive agent with significant antitumor and antiangiogenic properties, clinically approved for prevention of organ transplant rejection. METHODS: Twelve New Zealand albino rabbits were divided into four groups. Four different doses of rapamycin were prepared in 0.1 ml: 20 µg, 50 µg, 200 µg, and 1000 µg. Each concentration was injected in one eye of three rabbits, and 0.1 ml volume of sterile BSS was injected into the contralateral eye of the three rabbits. Slit-lamp and fundoscopic examinations were performed and the animals were observed for 2 weeks for signs of infection, inflammation, and toxicity. A baseline ERG was performed before drug treatment and at day 14, after which the rabbits were euthanized. Histology of the enucleated eyes was studied to look for retinal toxicity. RESULTS: ERG results showed some decrease in scotopic response; however this was not dose related. ERG results were normal at 20 µg. Histological results showed no retinal toxicity in all groups. CONCLUSION: Although ERG changes were identified at dosages between 50-1000 µg, the histology of all groups up to 1000 µg did not show any discernable abnormalities. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492009000100004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492009000100004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0004-27492009000100004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
dc.source.none.fl_str_mv |
Arquivos Brasileiros de Oftalmologia v.72 n.1 2009 reponame:Arquivos brasileiros de oftalmologia (Online) instname:Conselho Brasileiro de Oftalmologia (CBO) instacron:CBO |
instname_str |
Conselho Brasileiro de Oftalmologia (CBO) |
instacron_str |
CBO |
institution |
CBO |
reponame_str |
Arquivos brasileiros de oftalmologia (Online) |
collection |
Arquivos brasileiros de oftalmologia (Online) |
repository.name.fl_str_mv |
Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO) |
repository.mail.fl_str_mv |
aboonline@cbo.com.br||abo@cbo.com.br |
_version_ |
1754209025534197760 |