Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results

Detalhes bibliográficos
Autor(a) principal: Renesto,Adimara da Candelaria
Data de Publicação: 2012
Outros Autores: Barros,Jeison de Nadai, Campos,Mauro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos brasileiros de oftalmologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492012000200006
Resumo: PURPOSE: To report the impression cytologic results after corneal cross-linking and insertion of intrastromal corneal ring segments for keratoconus. METHODS: Thirty-nine eyes were distributed into two groups: 1) cross-linking group (patients underwent corneal cross-linking procedure), and 2) riboflavin eyedrops group (patients received 0.1% riboflavin (w/v) eyedrops in 20% dextran solution for 1 month). After 3 months, all patients underwent insertion of intrastromal corneal ring segments. Impression cytologic specimens were obtained from all eyes at baseline, at 1 month and 3 months after cross-linking or riboflavin eyedrops, and again at 6 months, 1 year, and 2 years after intrastromal corneal ring segment insertion. RESULTS: Patients in the cross-linking group demonstrated improvement in the cell-to-cell contact of epithelial cells and the nucleus-to-cytoplasm ratio on the temporal conjunctiva after treatment (P=0.008 and P=0.047), respectively. On the superior conjunctiva, increases in goblet cell density (P=0.037) and level of organization of nuclear chromatin (P=0.010) after treatment were noted. Patients in the riboflavin eyedrops group demonstrated improvement in the cell-to-cell contact of epithelial cells on the superior conjunctiva after treatment (P=0.021). On the temporal conjunctiva, an improvement in the cell-to-cell contact of epithelial cells (P<0.001) and increases in the nucleus-to-cytoplasm ratio (P<0.001), goblet cell density (P=0.001), and less keratinization (P=0.011) were noted. No changes were identified on the cornea for either group. Fisher's exact test comparison of the impression cytologic total scores after treatment revealed no difference between groups. CONCLUSION: Despite changes in some conjunctival parameters (e.g., cell-to-cell contact of epithelial cells, nucleus-to-cytoplasm ratio, level of organization of nuclear chromatin, goblet cell density, and keratinization), comparison of the total impression cytologic scores revealed no difference between groups.
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spelling Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year resultsCytological techniquesCorneaRiboflavinKeratoconusCorneal surgery, laserCorneal stromaPURPOSE: To report the impression cytologic results after corneal cross-linking and insertion of intrastromal corneal ring segments for keratoconus. METHODS: Thirty-nine eyes were distributed into two groups: 1) cross-linking group (patients underwent corneal cross-linking procedure), and 2) riboflavin eyedrops group (patients received 0.1% riboflavin (w/v) eyedrops in 20% dextran solution for 1 month). After 3 months, all patients underwent insertion of intrastromal corneal ring segments. Impression cytologic specimens were obtained from all eyes at baseline, at 1 month and 3 months after cross-linking or riboflavin eyedrops, and again at 6 months, 1 year, and 2 years after intrastromal corneal ring segment insertion. RESULTS: Patients in the cross-linking group demonstrated improvement in the cell-to-cell contact of epithelial cells and the nucleus-to-cytoplasm ratio on the temporal conjunctiva after treatment (P=0.008 and P=0.047), respectively. On the superior conjunctiva, increases in goblet cell density (P=0.037) and level of organization of nuclear chromatin (P=0.010) after treatment were noted. Patients in the riboflavin eyedrops group demonstrated improvement in the cell-to-cell contact of epithelial cells on the superior conjunctiva after treatment (P=0.021). On the temporal conjunctiva, an improvement in the cell-to-cell contact of epithelial cells (P<0.001) and increases in the nucleus-to-cytoplasm ratio (P<0.001), goblet cell density (P=0.001), and less keratinization (P=0.011) were noted. No changes were identified on the cornea for either group. Fisher's exact test comparison of the impression cytologic total scores after treatment revealed no difference between groups. CONCLUSION: Despite changes in some conjunctival parameters (e.g., cell-to-cell contact of epithelial cells, nucleus-to-cytoplasm ratio, level of organization of nuclear chromatin, goblet cell density, and keratinization), comparison of the total impression cytologic scores revealed no difference between groups.Conselho Brasileiro de Oftalmologia2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492012000200006Arquivos Brasileiros de Oftalmologia v.75 n.2 2012reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.1590/S0004-27492012000200006info:eu-repo/semantics/openAccessRenesto,Adimara da CandelariaBarros,Jeison de NadaiCampos,Mauroeng2012-06-15T00:00:00Zoai:scielo:S0004-27492012000200006Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2012-06-15T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false
dc.title.none.fl_str_mv Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
title Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
spellingShingle Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
Renesto,Adimara da Candelaria
Cytological techniques
Cornea
Riboflavin
Keratoconus
Corneal surgery, laser
Corneal stroma
title_short Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
title_full Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
title_fullStr Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
title_full_unstemmed Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
title_sort Impression cytologic analysis after corneal cross-linking and insertion of corneal ring segments for keratoconus: two-year results
author Renesto,Adimara da Candelaria
author_facet Renesto,Adimara da Candelaria
Barros,Jeison de Nadai
Campos,Mauro
author_role author
author2 Barros,Jeison de Nadai
Campos,Mauro
author2_role author
author
dc.contributor.author.fl_str_mv Renesto,Adimara da Candelaria
Barros,Jeison de Nadai
Campos,Mauro
dc.subject.por.fl_str_mv Cytological techniques
Cornea
Riboflavin
Keratoconus
Corneal surgery, laser
Corneal stroma
topic Cytological techniques
Cornea
Riboflavin
Keratoconus
Corneal surgery, laser
Corneal stroma
description PURPOSE: To report the impression cytologic results after corneal cross-linking and insertion of intrastromal corneal ring segments for keratoconus. METHODS: Thirty-nine eyes were distributed into two groups: 1) cross-linking group (patients underwent corneal cross-linking procedure), and 2) riboflavin eyedrops group (patients received 0.1% riboflavin (w/v) eyedrops in 20% dextran solution for 1 month). After 3 months, all patients underwent insertion of intrastromal corneal ring segments. Impression cytologic specimens were obtained from all eyes at baseline, at 1 month and 3 months after cross-linking or riboflavin eyedrops, and again at 6 months, 1 year, and 2 years after intrastromal corneal ring segment insertion. RESULTS: Patients in the cross-linking group demonstrated improvement in the cell-to-cell contact of epithelial cells and the nucleus-to-cytoplasm ratio on the temporal conjunctiva after treatment (P=0.008 and P=0.047), respectively. On the superior conjunctiva, increases in goblet cell density (P=0.037) and level of organization of nuclear chromatin (P=0.010) after treatment were noted. Patients in the riboflavin eyedrops group demonstrated improvement in the cell-to-cell contact of epithelial cells on the superior conjunctiva after treatment (P=0.021). On the temporal conjunctiva, an improvement in the cell-to-cell contact of epithelial cells (P<0.001) and increases in the nucleus-to-cytoplasm ratio (P<0.001), goblet cell density (P=0.001), and less keratinization (P=0.011) were noted. No changes were identified on the cornea for either group. Fisher's exact test comparison of the impression cytologic total scores after treatment revealed no difference between groups. CONCLUSION: Despite changes in some conjunctival parameters (e.g., cell-to-cell contact of epithelial cells, nucleus-to-cytoplasm ratio, level of organization of nuclear chromatin, goblet cell density, and keratinization), comparison of the total impression cytologic scores revealed no difference between groups.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
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dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
dc.source.none.fl_str_mv Arquivos Brasileiros de Oftalmologia v.75 n.2 2012
reponame:Arquivos brasileiros de oftalmologia (Online)
instname:Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO
instname_str Conselho Brasileiro de Oftalmologia (CBO)
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reponame_str Arquivos brasileiros de oftalmologia (Online)
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repository.name.fl_str_mv Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)
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