Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction

Detalhes bibliográficos
Autor(a) principal: Monteiro,Babyla Geraldes
Data de Publicação: 2019
Outros Autores: Loureiro,Renata Ruoco, Cristovam,Priscila Cardoso, Covre,Joyce Luciana, Gomes,José Álvaro Pereira, Kerkis,Irina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos brasileiros de oftalmologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492019000100032
Resumo: ABSTRACT Purpose: To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes. Methods: We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components. Results: Immature dental pulp stem cells grown on amniotic membranes under basal conditions are able to maintain their undifferentiated state. Our data also suggest that the culture medium used in the present work can modulate the expression of immature dental pulp stem cell markers, thus inducing epithelial differentiation of these cells in vitro. Conclusions: Our results suggest that the amniotic membrane is a good choice for the growth and transplantation of mesenchymal stem cells, particularly immature dental pulp stem cells, in clinical ocular surface reconstruction.
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spelling Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstructionCorneal epitheliaLimbus cornaeStem cellDental pulpAmniotic membraneOcular surfaceABSTRACT Purpose: To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes. Methods: We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components. Results: Immature dental pulp stem cells grown on amniotic membranes under basal conditions are able to maintain their undifferentiated state. Our data also suggest that the culture medium used in the present work can modulate the expression of immature dental pulp stem cell markers, thus inducing epithelial differentiation of these cells in vitro. Conclusions: Our results suggest that the amniotic membrane is a good choice for the growth and transplantation of mesenchymal stem cells, particularly immature dental pulp stem cells, in clinical ocular surface reconstruction.Conselho Brasileiro de Oftalmologia2019-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492019000100032Arquivos Brasileiros de Oftalmologia v.82 n.1 2019reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.5935/0004-2749.20190009info:eu-repo/semantics/openAccessMonteiro,Babyla GeraldesLoureiro,Renata RuocoCristovam,Priscila CardosoCovre,Joyce LucianaGomes,José Álvaro PereiraKerkis,Irinaeng2019-01-10T00:00:00Zoai:scielo:S0004-27492019000100032Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2019-01-10T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false
dc.title.none.fl_str_mv Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
title Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
spellingShingle Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
Monteiro,Babyla Geraldes
Corneal epithelia
Limbus cornae
Stem cell
Dental pulp
Amniotic membrane
Ocular surface
title_short Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
title_full Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
title_fullStr Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
title_full_unstemmed Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
title_sort Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
author Monteiro,Babyla Geraldes
author_facet Monteiro,Babyla Geraldes
Loureiro,Renata Ruoco
Cristovam,Priscila Cardoso
Covre,Joyce Luciana
Gomes,José Álvaro Pereira
Kerkis,Irina
author_role author
author2 Loureiro,Renata Ruoco
Cristovam,Priscila Cardoso
Covre,Joyce Luciana
Gomes,José Álvaro Pereira
Kerkis,Irina
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Monteiro,Babyla Geraldes
Loureiro,Renata Ruoco
Cristovam,Priscila Cardoso
Covre,Joyce Luciana
Gomes,José Álvaro Pereira
Kerkis,Irina
dc.subject.por.fl_str_mv Corneal epithelia
Limbus cornae
Stem cell
Dental pulp
Amniotic membrane
Ocular surface
topic Corneal epithelia
Limbus cornae
Stem cell
Dental pulp
Amniotic membrane
Ocular surface
description ABSTRACT Purpose: To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes. Methods: We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components. Results: Immature dental pulp stem cells grown on amniotic membranes under basal conditions are able to maintain their undifferentiated state. Our data also suggest that the culture medium used in the present work can modulate the expression of immature dental pulp stem cell markers, thus inducing epithelial differentiation of these cells in vitro. Conclusions: Our results suggest that the amniotic membrane is a good choice for the growth and transplantation of mesenchymal stem cells, particularly immature dental pulp stem cells, in clinical ocular surface reconstruction.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492019000100032
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492019000100032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0004-2749.20190009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
dc.source.none.fl_str_mv Arquivos Brasileiros de Oftalmologia v.82 n.1 2019
reponame:Arquivos brasileiros de oftalmologia (Online)
instname:Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO
instname_str Conselho Brasileiro de Oftalmologia (CBO)
instacron_str CBO
institution CBO
reponame_str Arquivos brasileiros de oftalmologia (Online)
collection Arquivos brasileiros de oftalmologia (Online)
repository.name.fl_str_mv Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)
repository.mail.fl_str_mv aboonline@cbo.com.br||abo@cbo.com.br
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