Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris

Detalhes bibliográficos
Autor(a) principal: Cunha,Rodrigo Casquero
Data de Publicação: 2011
Outros Autores: Andreotti,Renato, Leite,Fábio Pereira Leivas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000200003
Resumo: The cattle tick Rhipicephalus (Boophilus) microplus is responsible for great economic losses. It is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. Vaccines may help control this parasite, thereby reducing tick pesticide use. In this light, we performed subcloning of the gene of the protein Bm86-GC, the homologue protein that currently forms the basis of vaccines (GavacTM and TickGardPLUS) that have been developed against cattle ticks. The subcloning was done in the pPIC9 expression vector, for transformation in the yeast Pichia pastoris. This protein was characterized by expression of the recombinant Mut+ strain, which expressed greater quantities of protein. The expressed protein (rBm86-CG) was recognized in the Western-blot assay using anti-Gavac, anti-TickGard, anti-larval extract and anti-rBm86-CG polyclonal sera. The serum produced in cattle vaccinated with the antigen CG rBm86 presented high antibody titers and recognized the native protein. The rBm86-GC has potential relevance as an immunogen for vaccine formulation against cattle ticks.
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spelling Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastorisIxodesticksbovinesrBm86-CGimmunizationThe cattle tick Rhipicephalus (Boophilus) microplus is responsible for great economic losses. It is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. Vaccines may help control this parasite, thereby reducing tick pesticide use. In this light, we performed subcloning of the gene of the protein Bm86-GC, the homologue protein that currently forms the basis of vaccines (GavacTM and TickGardPLUS) that have been developed against cattle ticks. The subcloning was done in the pPIC9 expression vector, for transformation in the yeast Pichia pastoris. This protein was characterized by expression of the recombinant Mut+ strain, which expressed greater quantities of protein. The expressed protein (rBm86-CG) was recognized in the Western-blot assay using anti-Gavac, anti-TickGard, anti-larval extract and anti-rBm86-CG polyclonal sera. The serum produced in cattle vaccinated with the antigen CG rBm86 presented high antibody titers and recognized the native protein. The rBm86-GC has potential relevance as an immunogen for vaccine formulation against cattle ticks.Colégio Brasileiro de Parasitologia Veterinária2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000200003Revista Brasileira de Parasitologia Veterinária v.20 n.2 2011reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612011000200003info:eu-repo/semantics/openAccessCunha,Rodrigo CasqueroAndreotti,RenatoLeite,Fábio Pereira Leivaseng2011-07-07T00:00:00Zoai:scielo:S1984-29612011000200003Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2011-07-07T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
title Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
spellingShingle Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
Cunha,Rodrigo Casquero
Ixodes
ticks
bovines
rBm86-CG
immunization
title_short Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
title_full Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
title_fullStr Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
title_full_unstemmed Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
title_sort Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
author Cunha,Rodrigo Casquero
author_facet Cunha,Rodrigo Casquero
Andreotti,Renato
Leite,Fábio Pereira Leivas
author_role author
author2 Andreotti,Renato
Leite,Fábio Pereira Leivas
author2_role author
author
dc.contributor.author.fl_str_mv Cunha,Rodrigo Casquero
Andreotti,Renato
Leite,Fábio Pereira Leivas
dc.subject.por.fl_str_mv Ixodes
ticks
bovines
rBm86-CG
immunization
topic Ixodes
ticks
bovines
rBm86-CG
immunization
description The cattle tick Rhipicephalus (Boophilus) microplus is responsible for great economic losses. It is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. Vaccines may help control this parasite, thereby reducing tick pesticide use. In this light, we performed subcloning of the gene of the protein Bm86-GC, the homologue protein that currently forms the basis of vaccines (GavacTM and TickGardPLUS) that have been developed against cattle ticks. The subcloning was done in the pPIC9 expression vector, for transformation in the yeast Pichia pastoris. This protein was characterized by expression of the recombinant Mut+ strain, which expressed greater quantities of protein. The expressed protein (rBm86-CG) was recognized in the Western-blot assay using anti-Gavac, anti-TickGard, anti-larval extract and anti-rBm86-CG polyclonal sera. The serum produced in cattle vaccinated with the antigen CG rBm86 presented high antibody titers and recognized the native protein. The rBm86-GC has potential relevance as an immunogen for vaccine formulation against cattle ticks.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000200003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000200003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612011000200003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.20 n.2 2011
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
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reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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