Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs

Detalhes bibliográficos
Autor(a) principal: Pinheiro,Amanda Fernandes
Data de Publicação: 2015
Outros Autores: Borsuk,Sibele, Berne,Maria Elisabeth Aires, Pinto,Luciano da Silva, Andreotti,Renato, Roos,Talita, Roloff,Barbara Couto, Leite,Fábio Pereira Leivas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200148
Resumo: Neosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing neosporosis infection in sheep and dogs. We observed that the ELISA test yielded specificity of 94.5% and sensitivity of 100% for sheep and specificity of 93.3% and sensitivity of 100% for dogs. We observed that the sensitivity was higher than shown by the indirect fluorescent antibody test, and this was confirmed by means of Western blot. The results from this study suggest that the recombinant protein expressed in P. pastoris is a suitable antigen for use in immunodiagnosis to detect N. caninum in two important species exposed to this parasitosis.
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spelling Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogsNeospora caninumPichia pastorisNcSRS2ELISANeosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing neosporosis infection in sheep and dogs. We observed that the ELISA test yielded specificity of 94.5% and sensitivity of 100% for sheep and specificity of 93.3% and sensitivity of 100% for dogs. We observed that the sensitivity was higher than shown by the indirect fluorescent antibody test, and this was confirmed by means of Western blot. The results from this study suggest that the recombinant protein expressed in P. pastoris is a suitable antigen for use in immunodiagnosis to detect N. caninum in two important species exposed to this parasitosis.Colégio Brasileiro de Parasitologia Veterinária2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200148Revista Brasileira de Parasitologia Veterinária v.24 n.2 2015reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612015015info:eu-repo/semantics/openAccessPinheiro,Amanda FernandesBorsuk,SibeleBerne,Maria Elisabeth AiresPinto,Luciano da SilvaAndreotti,RenatoRoos,TalitaRoloff,Barbara CoutoLeite,Fábio Pereira Leivaseng2015-10-09T00:00:00Zoai:scielo:S1984-29612015000200148Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-10-09T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
title Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
spellingShingle Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
Pinheiro,Amanda Fernandes
Neospora caninum
Pichia pastoris
NcSRS2
ELISA
title_short Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
title_full Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
title_fullStr Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
title_full_unstemmed Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
title_sort Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
author Pinheiro,Amanda Fernandes
author_facet Pinheiro,Amanda Fernandes
Borsuk,Sibele
Berne,Maria Elisabeth Aires
Pinto,Luciano da Silva
Andreotti,Renato
Roos,Talita
Roloff,Barbara Couto
Leite,Fábio Pereira Leivas
author_role author
author2 Borsuk,Sibele
Berne,Maria Elisabeth Aires
Pinto,Luciano da Silva
Andreotti,Renato
Roos,Talita
Roloff,Barbara Couto
Leite,Fábio Pereira Leivas
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro,Amanda Fernandes
Borsuk,Sibele
Berne,Maria Elisabeth Aires
Pinto,Luciano da Silva
Andreotti,Renato
Roos,Talita
Roloff,Barbara Couto
Leite,Fábio Pereira Leivas
dc.subject.por.fl_str_mv Neospora caninum
Pichia pastoris
NcSRS2
ELISA
topic Neospora caninum
Pichia pastoris
NcSRS2
ELISA
description Neosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing neosporosis infection in sheep and dogs. We observed that the ELISA test yielded specificity of 94.5% and sensitivity of 100% for sheep and specificity of 93.3% and sensitivity of 100% for dogs. We observed that the sensitivity was higher than shown by the indirect fluorescent antibody test, and this was confirmed by means of Western blot. The results from this study suggest that the recombinant protein expressed in P. pastoris is a suitable antigen for use in immunodiagnosis to detect N. caninum in two important species exposed to this parasitosis.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200148
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200148
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612015015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.24 n.2 2015
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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