Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400318 |
Resumo: | Abstract Neospora caninum is an obligate intracellular parasite that can infect many domestic and wild animals, including birds. These animals are important sources for monitoring of environmental contamination, as they could become infected through sporulated oocysts; however, the real role of birds in the biological cycle of N. caninum remains uncertain. This study aimed to determine the prevalence of anti-N. caninum antibodies, evaluate associated factors, detect the parasite by molecular testing of free-range chickens from Brazil, and evaluate different techniques for its serological diagnosis. Blood samples of 366 chickens from 25 farms were collected for serological assays. The indirect fluorescent antibody test (IFAT) and the indirect enzyme-linked immunosorbent assay (ELISA) were used to detect anti-N. caninum antibodies. Chickens that tested seropositive by IFAT had their brain tissues and a pool of organs (heart, lung, and liver) submitted to PCR for molecular detection of the parasite. Out of 366 chickens, 65 (17.8%) and 163 (44.6%) were seropositive by IFAT and ELISA, respectively. Brain tissues (n=60) and the pools of organs (n=65) were negative in the PCR. Our results showed a high prevalence of antibodies in free-range chickens and that IFAT is the more sensitive technique for the detection of anti-N. caninum antibodies. |
id |
CBPV-1_22ff80280c34245c0cb025c77a9f6485 |
---|---|
oai_identifier_str |
oai:scielo:S1984-29612020000400318 |
network_acronym_str |
CBPV-1 |
network_name_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository_id_str |
|
spelling |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern BrazilFree-range chickenindirect fluorescent antibody testenzyme-linked immunosorbent assaypolymerase chain reactionAbstract Neospora caninum is an obligate intracellular parasite that can infect many domestic and wild animals, including birds. These animals are important sources for monitoring of environmental contamination, as they could become infected through sporulated oocysts; however, the real role of birds in the biological cycle of N. caninum remains uncertain. This study aimed to determine the prevalence of anti-N. caninum antibodies, evaluate associated factors, detect the parasite by molecular testing of free-range chickens from Brazil, and evaluate different techniques for its serological diagnosis. Blood samples of 366 chickens from 25 farms were collected for serological assays. The indirect fluorescent antibody test (IFAT) and the indirect enzyme-linked immunosorbent assay (ELISA) were used to detect anti-N. caninum antibodies. Chickens that tested seropositive by IFAT had their brain tissues and a pool of organs (heart, lung, and liver) submitted to PCR for molecular detection of the parasite. Out of 366 chickens, 65 (17.8%) and 163 (44.6%) were seropositive by IFAT and ELISA, respectively. Brain tissues (n=60) and the pools of organs (n=65) were negative in the PCR. Our results showed a high prevalence of antibodies in free-range chickens and that IFAT is the more sensitive technique for the detection of anti-N. caninum antibodies.Colégio Brasileiro de Parasitologia Veterinária2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400318Revista Brasileira de Parasitologia Veterinária v.29 n.4 2020reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612020107info:eu-repo/semantics/openAccessSasse,João PedroSilva,Ana Clécia dos SantosCarneiro,Priscilla GomesNino,Beatriz de Souza LimaVieira,Fernando Emmanuel GonçalvesBarros,Luiz Daniel deGarcia,João Luiseng2020-11-30T00:00:00Zoai:scielo:S1984-29612020000400318Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2020-11-30T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil |
title |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil |
spellingShingle |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil Sasse,João Pedro Free-range chicken indirect fluorescent antibody test enzyme-linked immunosorbent assay polymerase chain reaction |
title_short |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil |
title_full |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil |
title_fullStr |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil |
title_full_unstemmed |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil |
title_sort |
Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil |
author |
Sasse,João Pedro |
author_facet |
Sasse,João Pedro Silva,Ana Clécia dos Santos Carneiro,Priscilla Gomes Nino,Beatriz de Souza Lima Vieira,Fernando Emmanuel Gonçalves Barros,Luiz Daniel de Garcia,João Luis |
author_role |
author |
author2 |
Silva,Ana Clécia dos Santos Carneiro,Priscilla Gomes Nino,Beatriz de Souza Lima Vieira,Fernando Emmanuel Gonçalves Barros,Luiz Daniel de Garcia,João Luis |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sasse,João Pedro Silva,Ana Clécia dos Santos Carneiro,Priscilla Gomes Nino,Beatriz de Souza Lima Vieira,Fernando Emmanuel Gonçalves Barros,Luiz Daniel de Garcia,João Luis |
dc.subject.por.fl_str_mv |
Free-range chicken indirect fluorescent antibody test enzyme-linked immunosorbent assay polymerase chain reaction |
topic |
Free-range chicken indirect fluorescent antibody test enzyme-linked immunosorbent assay polymerase chain reaction |
description |
Abstract Neospora caninum is an obligate intracellular parasite that can infect many domestic and wild animals, including birds. These animals are important sources for monitoring of environmental contamination, as they could become infected through sporulated oocysts; however, the real role of birds in the biological cycle of N. caninum remains uncertain. This study aimed to determine the prevalence of anti-N. caninum antibodies, evaluate associated factors, detect the parasite by molecular testing of free-range chickens from Brazil, and evaluate different techniques for its serological diagnosis. Blood samples of 366 chickens from 25 farms were collected for serological assays. The indirect fluorescent antibody test (IFAT) and the indirect enzyme-linked immunosorbent assay (ELISA) were used to detect anti-N. caninum antibodies. Chickens that tested seropositive by IFAT had their brain tissues and a pool of organs (heart, lung, and liver) submitted to PCR for molecular detection of the parasite. Out of 366 chickens, 65 (17.8%) and 163 (44.6%) were seropositive by IFAT and ELISA, respectively. Brain tissues (n=60) and the pools of organs (n=65) were negative in the PCR. Our results showed a high prevalence of antibodies in free-range chickens and that IFAT is the more sensitive technique for the detection of anti-N. caninum antibodies. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400318 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400318 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-29612020107 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.29 n.4 2020 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
_version_ |
1754208918831104000 |