Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil

Detalhes bibliográficos
Autor(a) principal: Sasse,João Pedro
Data de Publicação: 2020
Outros Autores: Silva,Ana Clécia dos Santos, Carneiro,Priscilla Gomes, Nino,Beatriz de Souza Lima, Vieira,Fernando Emmanuel Gonçalves, Barros,Luiz Daniel de, Garcia,João Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400318
Resumo: Abstract Neospora caninum is an obligate intracellular parasite that can infect many domestic and wild animals, including birds. These animals are important sources for monitoring of environmental contamination, as they could become infected through sporulated oocysts; however, the real role of birds in the biological cycle of N. caninum remains uncertain. This study aimed to determine the prevalence of anti-N. caninum antibodies, evaluate associated factors, detect the parasite by molecular testing of free-range chickens from Brazil, and evaluate different techniques for its serological diagnosis. Blood samples of 366 chickens from 25 farms were collected for serological assays. The indirect fluorescent antibody test (IFAT) and the indirect enzyme-linked immunosorbent assay (ELISA) were used to detect anti-N. caninum antibodies. Chickens that tested seropositive by IFAT had their brain tissues and a pool of organs (heart, lung, and liver) submitted to PCR for molecular detection of the parasite. Out of 366 chickens, 65 (17.8%) and 163 (44.6%) were seropositive by IFAT and ELISA, respectively. Brain tissues (n=60) and the pools of organs (n=65) were negative in the PCR. Our results showed a high prevalence of antibodies in free-range chickens and that IFAT is the more sensitive technique for the detection of anti-N. caninum antibodies.
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spelling Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern BrazilFree-range chickenindirect fluorescent antibody testenzyme-linked immunosorbent assaypolymerase chain reactionAbstract Neospora caninum is an obligate intracellular parasite that can infect many domestic and wild animals, including birds. These animals are important sources for monitoring of environmental contamination, as they could become infected through sporulated oocysts; however, the real role of birds in the biological cycle of N. caninum remains uncertain. This study aimed to determine the prevalence of anti-N. caninum antibodies, evaluate associated factors, detect the parasite by molecular testing of free-range chickens from Brazil, and evaluate different techniques for its serological diagnosis. Blood samples of 366 chickens from 25 farms were collected for serological assays. The indirect fluorescent antibody test (IFAT) and the indirect enzyme-linked immunosorbent assay (ELISA) were used to detect anti-N. caninum antibodies. Chickens that tested seropositive by IFAT had their brain tissues and a pool of organs (heart, lung, and liver) submitted to PCR for molecular detection of the parasite. Out of 366 chickens, 65 (17.8%) and 163 (44.6%) were seropositive by IFAT and ELISA, respectively. Brain tissues (n=60) and the pools of organs (n=65) were negative in the PCR. Our results showed a high prevalence of antibodies in free-range chickens and that IFAT is the more sensitive technique for the detection of anti-N. caninum antibodies.Colégio Brasileiro de Parasitologia Veterinária2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400318Revista Brasileira de Parasitologia Veterinária v.29 n.4 2020reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612020107info:eu-repo/semantics/openAccessSasse,João PedroSilva,Ana Clécia dos SantosCarneiro,Priscilla GomesNino,Beatriz de Souza LimaVieira,Fernando Emmanuel GonçalvesBarros,Luiz Daniel deGarcia,João Luiseng2020-11-30T00:00:00Zoai:scielo:S1984-29612020000400318Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2020-11-30T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
title Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
spellingShingle Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
Sasse,João Pedro
Free-range chicken
indirect fluorescent antibody test
enzyme-linked immunosorbent assay
polymerase chain reaction
title_short Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
title_full Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
title_fullStr Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
title_full_unstemmed Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
title_sort Neospora caninum in free-range chickens (Gallus gallus domesticus) from southern Brazil
author Sasse,João Pedro
author_facet Sasse,João Pedro
Silva,Ana Clécia dos Santos
Carneiro,Priscilla Gomes
Nino,Beatriz de Souza Lima
Vieira,Fernando Emmanuel Gonçalves
Barros,Luiz Daniel de
Garcia,João Luis
author_role author
author2 Silva,Ana Clécia dos Santos
Carneiro,Priscilla Gomes
Nino,Beatriz de Souza Lima
Vieira,Fernando Emmanuel Gonçalves
Barros,Luiz Daniel de
Garcia,João Luis
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sasse,João Pedro
Silva,Ana Clécia dos Santos
Carneiro,Priscilla Gomes
Nino,Beatriz de Souza Lima
Vieira,Fernando Emmanuel Gonçalves
Barros,Luiz Daniel de
Garcia,João Luis
dc.subject.por.fl_str_mv Free-range chicken
indirect fluorescent antibody test
enzyme-linked immunosorbent assay
polymerase chain reaction
topic Free-range chicken
indirect fluorescent antibody test
enzyme-linked immunosorbent assay
polymerase chain reaction
description Abstract Neospora caninum is an obligate intracellular parasite that can infect many domestic and wild animals, including birds. These animals are important sources for monitoring of environmental contamination, as they could become infected through sporulated oocysts; however, the real role of birds in the biological cycle of N. caninum remains uncertain. This study aimed to determine the prevalence of anti-N. caninum antibodies, evaluate associated factors, detect the parasite by molecular testing of free-range chickens from Brazil, and evaluate different techniques for its serological diagnosis. Blood samples of 366 chickens from 25 farms were collected for serological assays. The indirect fluorescent antibody test (IFAT) and the indirect enzyme-linked immunosorbent assay (ELISA) were used to detect anti-N. caninum antibodies. Chickens that tested seropositive by IFAT had their brain tissues and a pool of organs (heart, lung, and liver) submitted to PCR for molecular detection of the parasite. Out of 366 chickens, 65 (17.8%) and 163 (44.6%) were seropositive by IFAT and ELISA, respectively. Brain tissues (n=60) and the pools of organs (n=65) were negative in the PCR. Our results showed a high prevalence of antibodies in free-range chickens and that IFAT is the more sensitive technique for the detection of anti-N. caninum antibodies.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400318
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1984-29612020107
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.29 n.4 2020
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
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reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
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repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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