Microscopic alterations in Fasciola hepatica from sheep treated with albendazole

Detalhes bibliográficos
Autor(a) principal: Carneiro,Milena Batista
Data de Publicação: 2019
Outros Autores: Avelar,Barbara Rauta, Archanjo,Anderson Barros, Martins,Isabella Vilhena Freire, Nunes,Louisiane de Carvalho, Scott,Fabio Barbour
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000100033
Resumo: Abstract Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours after treatment. The results indicated that parasitic spermatogenesis was severely affected by albendazole and demonstrated the importance of the use of histopathology for the diagnosis of therapeutic efficacy in field strains.
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spelling Microscopic alterations in Fasciola hepatica from sheep treated with albendazoleFasciolosishistopathologytreatmentalbendazoleAbstract Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours after treatment. The results indicated that parasitic spermatogenesis was severely affected by albendazole and demonstrated the importance of the use of histopathology for the diagnosis of therapeutic efficacy in field strains.Colégio Brasileiro de Parasitologia Veterinária2019-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000100033Revista Brasileira de Parasitologia Veterinária v.28 n.1 2019reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-296120180095info:eu-repo/semantics/openAccessCarneiro,Milena BatistaAvelar,Barbara RautaArchanjo,Anderson BarrosMartins,Isabella Vilhena FreireNunes,Louisiane de CarvalhoScott,Fabio Barboureng2020-01-28T00:00:00Zoai:scielo:S1984-29612019000100033Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2020-01-28T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Microscopic alterations in Fasciola hepatica from sheep treated with albendazole
title Microscopic alterations in Fasciola hepatica from sheep treated with albendazole
spellingShingle Microscopic alterations in Fasciola hepatica from sheep treated with albendazole
Carneiro,Milena Batista
Fasciolosis
histopathology
treatment
albendazole
title_short Microscopic alterations in Fasciola hepatica from sheep treated with albendazole
title_full Microscopic alterations in Fasciola hepatica from sheep treated with albendazole
title_fullStr Microscopic alterations in Fasciola hepatica from sheep treated with albendazole
title_full_unstemmed Microscopic alterations in Fasciola hepatica from sheep treated with albendazole
title_sort Microscopic alterations in Fasciola hepatica from sheep treated with albendazole
author Carneiro,Milena Batista
author_facet Carneiro,Milena Batista
Avelar,Barbara Rauta
Archanjo,Anderson Barros
Martins,Isabella Vilhena Freire
Nunes,Louisiane de Carvalho
Scott,Fabio Barbour
author_role author
author2 Avelar,Barbara Rauta
Archanjo,Anderson Barros
Martins,Isabella Vilhena Freire
Nunes,Louisiane de Carvalho
Scott,Fabio Barbour
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carneiro,Milena Batista
Avelar,Barbara Rauta
Archanjo,Anderson Barros
Martins,Isabella Vilhena Freire
Nunes,Louisiane de Carvalho
Scott,Fabio Barbour
dc.subject.por.fl_str_mv Fasciolosis
histopathology
treatment
albendazole
topic Fasciolosis
histopathology
treatment
albendazole
description Abstract Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours after treatment. The results indicated that parasitic spermatogenesis was severely affected by albendazole and demonstrated the importance of the use of histopathology for the diagnosis of therapeutic efficacy in field strains.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000100033
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000100033
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1984-296120180095
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.28 n.1 2019
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
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