Anthelmintic activity of nanoencapsulated carvacryl acetate against gastrointestinal nematodes of sheep and its toxicity in rodents

• Autor(a) principal: André,Weibson Paz Pinheiro
• Data de Publicação: 2020
• Outros Autores: Paiva Junior,José Ribamar de, Cavalcante,Géssica Soares, Ribeiro,Wesley Lyeverton Correia, Araújo Filho,José Vilemar de, Santos,Jéssica Maria Leite dos, Alves,Ana Paula Negreiros Nunes, Monteiro,Jomar Patrício, Morais,Selene Maia de, Silva,Isaac Neto Goes da, Oliveira,Lorena Mayana Beserra de, Abreu,Flávia Oliveira Monteiro da Silva, Bevilaqua,Claudia Maria Leal
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000100300
• topic: Chitosan; gum arabic; biocompounds; acetylation; toxicological safety; Haemonchus contortus

Abstract The objective of this study was to evaluate the efficacy of carvacryl acetate (CVA) and nanoencapsulated CVA (nCVA) on gastrointestinal nematodes of sheep. The CVA was nanoencapsulated with chitosan/gum arabic and the efficacy of nanoencapsulation (EE), yield, zeta potential, nanoparticle morphology and release kinetics at pH 3 and 8 were analyzed. Acute and subchronic toxicity were evaluated in rodents and reduction of egg counts in the faeces (FECRT) of sheep. The sheep were divided into four groups (n = 10): G1, 250 mg/kg CVA; G2, 250 mg/kg nCVA; G3, polymer matrix and G4: 2.5 mg/kg monepantel. EE and nCVA yield were 65% and 57%, respectively. The morphology of the nanoparticles was spherical, size (810.6±286.7 nm), zeta potential in pH 3.2 (+18.3 mV) and the 50% release of CVA at pHs 3 and 8 occurred at 200 and 10 h, respectively. nCVA showed LD50 of 2,609 mg/kg. CVA, nCVA and monepantel reduced the number of eggs per gram of faeces (epg) by 57.7%, 51.1% and 97.7%, respectively. The epg of sheep treated with CVA and nCVA did not differ from the negative control (P>0.05). Nanoencapsulation reduced the toxicity of CVA; however, nCVA and CVA presented similar results in the FECRT.