Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture

Detalhes bibliográficos
Autor(a) principal: Lasmar,Pedro Veloso Facury
Data de Publicação: 2012
Outros Autores: Carvalho,Antônio Último de, Facury Filho,Elias Jorge, Bastos,Camila Valgas, Ribeiro,Múcio Flávio Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200008
Resumo: The protective efficacy of an inactivated vaccine from Anaplasma marginale that was cultured in tick cells (IDE8) for use against bovine anaplasmosis was evaluated. Five calves (Group 1) were inoculated subcutaneously, at 21-day intervals, with three doses of vaccine containing 3 × 10(9) A. marginale initial bodies. Five control calves received saline solution alone (Group 2). Thirty-two days after the final inoculation, all the calves were challenged with approximately 3 × 10(5) erythrocytes infected with A. marginale high-virulence isolate (UFMG2). The Group 1 calves seroconverted 14 days after the second dose of vaccine. After the challenge, all the animals showed patent rickettsemia. There was no significant difference (p > 0.05) between the Group 1 and 2 calves during the incubation period, patency period or convalescence period. All the animals required treatment to prevent death. The results suggest that the inactivated vaccine from A. marginale produced in IDE8 induced seroconversion in calves, but was not effective for preventing anaplasmosis induced by the UFMG2 isolate under the conditions of this experiment.
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spelling Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell cultureAnaplasma marginalevaccinationinactivated vaccineIDE8The protective efficacy of an inactivated vaccine from Anaplasma marginale that was cultured in tick cells (IDE8) for use against bovine anaplasmosis was evaluated. Five calves (Group 1) were inoculated subcutaneously, at 21-day intervals, with three doses of vaccine containing 3 × 10(9) A. marginale initial bodies. Five control calves received saline solution alone (Group 2). Thirty-two days after the final inoculation, all the calves were challenged with approximately 3 × 10(5) erythrocytes infected with A. marginale high-virulence isolate (UFMG2). The Group 1 calves seroconverted 14 days after the second dose of vaccine. After the challenge, all the animals showed patent rickettsemia. There was no significant difference (p > 0.05) between the Group 1 and 2 calves during the incubation period, patency period or convalescence period. All the animals required treatment to prevent death. The results suggest that the inactivated vaccine from A. marginale produced in IDE8 induced seroconversion in calves, but was not effective for preventing anaplasmosis induced by the UFMG2 isolate under the conditions of this experiment.Colégio Brasileiro de Parasitologia Veterinária2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200008Revista Brasileira de Parasitologia Veterinária v.21 n.2 2012reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612012000200008info:eu-repo/semantics/openAccessLasmar,Pedro Veloso FacuryCarvalho,Antônio Último deFacury Filho,Elias JorgeBastos,Camila ValgasRibeiro,Múcio Flávio Barbosaeng2012-07-19T00:00:00Zoai:scielo:S1984-29612012000200008Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2012-07-19T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
title Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
spellingShingle Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
Lasmar,Pedro Veloso Facury
Anaplasma marginale
vaccination
inactivated vaccine
IDE8
title_short Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
title_full Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
title_fullStr Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
title_full_unstemmed Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
title_sort Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
author Lasmar,Pedro Veloso Facury
author_facet Lasmar,Pedro Veloso Facury
Carvalho,Antônio Último de
Facury Filho,Elias Jorge
Bastos,Camila Valgas
Ribeiro,Múcio Flávio Barbosa
author_role author
author2 Carvalho,Antônio Último de
Facury Filho,Elias Jorge
Bastos,Camila Valgas
Ribeiro,Múcio Flávio Barbosa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lasmar,Pedro Veloso Facury
Carvalho,Antônio Último de
Facury Filho,Elias Jorge
Bastos,Camila Valgas
Ribeiro,Múcio Flávio Barbosa
dc.subject.por.fl_str_mv Anaplasma marginale
vaccination
inactivated vaccine
IDE8
topic Anaplasma marginale
vaccination
inactivated vaccine
IDE8
description The protective efficacy of an inactivated vaccine from Anaplasma marginale that was cultured in tick cells (IDE8) for use against bovine anaplasmosis was evaluated. Five calves (Group 1) were inoculated subcutaneously, at 21-day intervals, with three doses of vaccine containing 3 × 10(9) A. marginale initial bodies. Five control calves received saline solution alone (Group 2). Thirty-two days after the final inoculation, all the calves were challenged with approximately 3 × 10(5) erythrocytes infected with A. marginale high-virulence isolate (UFMG2). The Group 1 calves seroconverted 14 days after the second dose of vaccine. After the challenge, all the animals showed patent rickettsemia. There was no significant difference (p > 0.05) between the Group 1 and 2 calves during the incubation period, patency period or convalescence period. All the animals required treatment to prevent death. The results suggest that the inactivated vaccine from A. marginale produced in IDE8 induced seroconversion in calves, but was not effective for preventing anaplasmosis induced by the UFMG2 isolate under the conditions of this experiment.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612012000200008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.21 n.2 2012
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
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reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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