Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia

Detalhes bibliográficos
Autor(a) principal: Colle,Ana Cláudia
Data de Publicação: 2019
Outros Autores: Mendonça,Ravena Fernanda Braga de, Maia,Maerle Oliveira, Freitas,Leodil da Costa, Witter,Rute, Marcili,Arlei, Aguiar,Daniel Moura de, Muñoz-Leal,Sebastián, Labruna,Marcelo Bahia, Rossi,Rogério Vieira, Pacheco,Richard de Campos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400592
Resumo: Abstract Small non-volant mammals (marsupials and small rodents) were captured at three different timepoints from 23 forest fragments across three municipalities (Alta Floresta, Sinop and Cláudia) covering the Amazonian biome of the Mato Grosso State in Midwestern Brazil. The animal tissues (liver and spleen) and blood were screened using molecular tools for the detection of Babesia, Coxiella, Cytauxzoon, Hepatozoon, Theileria, and Anaplasmataceae agents. A total of 230 specimens (78 rodents and 152 marsupials) were trapped. Hepatozoon and Piroplasmorida agents were detected in the common opossums (Didelphis marsupialis). In turn, all samples (blood, liver, or spleen) collected from the small mammals were negative for the genus Coxiella and the family Anaplasmataceae, as detected by polymerase chain reaction (PCR). Phylogenetic analyses inferred from partial sequences of the 18S rRNA gene highlighted the occurrence of new Hepatozoon and Piroplasmorida haplotypes. Future studies determining the role of common opossum (D. marsupialis) in the epidemiological cycles of Hepatozoon and Babesia under natural conditions in the Amazonian biome are necessary.
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spelling Molecular survey of tick-borne pathogens in small mammals from Brazilian AmazoniaHepatozoonBabesiaAmazonian biomeMato Grosso stateAbstract Small non-volant mammals (marsupials and small rodents) were captured at three different timepoints from 23 forest fragments across three municipalities (Alta Floresta, Sinop and Cláudia) covering the Amazonian biome of the Mato Grosso State in Midwestern Brazil. The animal tissues (liver and spleen) and blood were screened using molecular tools for the detection of Babesia, Coxiella, Cytauxzoon, Hepatozoon, Theileria, and Anaplasmataceae agents. A total of 230 specimens (78 rodents and 152 marsupials) were trapped. Hepatozoon and Piroplasmorida agents were detected in the common opossums (Didelphis marsupialis). In turn, all samples (blood, liver, or spleen) collected from the small mammals were negative for the genus Coxiella and the family Anaplasmataceae, as detected by polymerase chain reaction (PCR). Phylogenetic analyses inferred from partial sequences of the 18S rRNA gene highlighted the occurrence of new Hepatozoon and Piroplasmorida haplotypes. Future studies determining the role of common opossum (D. marsupialis) in the epidemiological cycles of Hepatozoon and Babesia under natural conditions in the Amazonian biome are necessary.Colégio Brasileiro de Parasitologia Veterinária2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400592Revista Brasileira de Parasitologia Veterinária v.28 n.4 2019reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612019086info:eu-repo/semantics/openAccessColle,Ana CláudiaMendonça,Ravena Fernanda Braga deMaia,Maerle OliveiraFreitas,Leodil da CostaWitter,RuteMarcili,ArleiAguiar,Daniel Moura deMuñoz-Leal,SebastiánLabruna,Marcelo BahiaRossi,Rogério VieiraPacheco,Richard de Camposeng2020-04-14T00:00:00Zoai:scielo:S1984-29612019000400592Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2020-04-14T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
title Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
spellingShingle Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
Colle,Ana Cláudia
Hepatozoon
Babesia
Amazonian biome
Mato Grosso state
title_short Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
title_full Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
title_fullStr Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
title_full_unstemmed Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
title_sort Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
author Colle,Ana Cláudia
author_facet Colle,Ana Cláudia
Mendonça,Ravena Fernanda Braga de
Maia,Maerle Oliveira
Freitas,Leodil da Costa
Witter,Rute
Marcili,Arlei
Aguiar,Daniel Moura de
Muñoz-Leal,Sebastián
Labruna,Marcelo Bahia
Rossi,Rogério Vieira
Pacheco,Richard de Campos
author_role author
author2 Mendonça,Ravena Fernanda Braga de
Maia,Maerle Oliveira
Freitas,Leodil da Costa
Witter,Rute
Marcili,Arlei
Aguiar,Daniel Moura de
Muñoz-Leal,Sebastián
Labruna,Marcelo Bahia
Rossi,Rogério Vieira
Pacheco,Richard de Campos
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Colle,Ana Cláudia
Mendonça,Ravena Fernanda Braga de
Maia,Maerle Oliveira
Freitas,Leodil da Costa
Witter,Rute
Marcili,Arlei
Aguiar,Daniel Moura de
Muñoz-Leal,Sebastián
Labruna,Marcelo Bahia
Rossi,Rogério Vieira
Pacheco,Richard de Campos
dc.subject.por.fl_str_mv Hepatozoon
Babesia
Amazonian biome
Mato Grosso state
topic Hepatozoon
Babesia
Amazonian biome
Mato Grosso state
description Abstract Small non-volant mammals (marsupials and small rodents) were captured at three different timepoints from 23 forest fragments across three municipalities (Alta Floresta, Sinop and Cláudia) covering the Amazonian biome of the Mato Grosso State in Midwestern Brazil. The animal tissues (liver and spleen) and blood were screened using molecular tools for the detection of Babesia, Coxiella, Cytauxzoon, Hepatozoon, Theileria, and Anaplasmataceae agents. A total of 230 specimens (78 rodents and 152 marsupials) were trapped. Hepatozoon and Piroplasmorida agents were detected in the common opossums (Didelphis marsupialis). In turn, all samples (blood, liver, or spleen) collected from the small mammals were negative for the genus Coxiella and the family Anaplasmataceae, as detected by polymerase chain reaction (PCR). Phylogenetic analyses inferred from partial sequences of the 18S rRNA gene highlighted the occurrence of new Hepatozoon and Piroplasmorida haplotypes. Future studies determining the role of common opossum (D. marsupialis) in the epidemiological cycles of Hepatozoon and Babesia under natural conditions in the Amazonian biome are necessary.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400592
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400592
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1984-29612019086
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.28 n.4 2019
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
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instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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