Humoral response and evolution of Echinococcus infection in experimentally infected sheep
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000200237 |
Resumo: | There is little information on the humoral response of sheep experimentally infected with Echinococcus granulosus. Thus, the objective of this study was to evaluate this response and measure its evolution. Doses of 10, 100, 1000 and 10000 E. granulosus eggs were prepared and inoculated via intraruminal puncture. Blood samples were obtained before inoculation and every 48 h after inoculation, until they became seropositive. Thereafter, they were taken monthly for the first year and then every three months until 1700 days of observation had been completed. An ELISA test, with total hydatid fluid antigen, was used for immunodiagnosis. The average optical density of the 12 inoculated sheep was found to be above the mean cutoff value 10 days after inoculation, went on increasing until 180 days after inoculation and remained above the cutoff level until the end of the observation period. This confirms that the antibody response of sheep to E. granulosus infection occurs before production of hydatid fluid and that activation, mobilization and establishment of oncospheres in the tissues generates a persistent response from the host's immune system. |
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Humoral response and evolution of Echinococcus infection in experimentally infected sheepEchinococcosishumoral responsesheepThere is little information on the humoral response of sheep experimentally infected with Echinococcus granulosus. Thus, the objective of this study was to evaluate this response and measure its evolution. Doses of 10, 100, 1000 and 10000 E. granulosus eggs were prepared and inoculated via intraruminal puncture. Blood samples were obtained before inoculation and every 48 h after inoculation, until they became seropositive. Thereafter, they were taken monthly for the first year and then every three months until 1700 days of observation had been completed. An ELISA test, with total hydatid fluid antigen, was used for immunodiagnosis. The average optical density of the 12 inoculated sheep was found to be above the mean cutoff value 10 days after inoculation, went on increasing until 180 days after inoculation and remained above the cutoff level until the end of the observation period. This confirms that the antibody response of sheep to E. granulosus infection occurs before production of hydatid fluid and that activation, mobilization and establishment of oncospheres in the tissues generates a persistent response from the host's immune system.Colégio Brasileiro de Parasitologia Veterinária2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000200237Revista Brasileira de Parasitologia Veterinária v.23 n.2 2014reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612014030info:eu-repo/semantics/openAccessLamberti,RobertoCavagion,LauraGatti,AntonioCalvo,ClaudioGino,LiliaPuches,Veronica VéspoliAlvarez,Angela RosaAlvarez,EmilianoCachau,Mariela GarcíaMorete,MarielaLarrieu,Edmundoeng2015-09-23T00:00:00Zoai:scielo:S1984-29612014000200237Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-09-23T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Humoral response and evolution of Echinococcus infection in experimentally infected sheep |
title |
Humoral response and evolution of Echinococcus infection in experimentally infected sheep |
spellingShingle |
Humoral response and evolution of Echinococcus infection in experimentally infected sheep Lamberti,Roberto Echinococcosis humoral response sheep |
title_short |
Humoral response and evolution of Echinococcus infection in experimentally infected sheep |
title_full |
Humoral response and evolution of Echinococcus infection in experimentally infected sheep |
title_fullStr |
Humoral response and evolution of Echinococcus infection in experimentally infected sheep |
title_full_unstemmed |
Humoral response and evolution of Echinococcus infection in experimentally infected sheep |
title_sort |
Humoral response and evolution of Echinococcus infection in experimentally infected sheep |
author |
Lamberti,Roberto |
author_facet |
Lamberti,Roberto Cavagion,Laura Gatti,Antonio Calvo,Claudio Gino,Lilia Puches,Veronica Véspoli Alvarez,Angela Rosa Alvarez,Emiliano Cachau,Mariela García Morete,Mariela Larrieu,Edmundo |
author_role |
author |
author2 |
Cavagion,Laura Gatti,Antonio Calvo,Claudio Gino,Lilia Puches,Veronica Véspoli Alvarez,Angela Rosa Alvarez,Emiliano Cachau,Mariela García Morete,Mariela Larrieu,Edmundo |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lamberti,Roberto Cavagion,Laura Gatti,Antonio Calvo,Claudio Gino,Lilia Puches,Veronica Véspoli Alvarez,Angela Rosa Alvarez,Emiliano Cachau,Mariela García Morete,Mariela Larrieu,Edmundo |
dc.subject.por.fl_str_mv |
Echinococcosis humoral response sheep |
topic |
Echinococcosis humoral response sheep |
description |
There is little information on the humoral response of sheep experimentally infected with Echinococcus granulosus. Thus, the objective of this study was to evaluate this response and measure its evolution. Doses of 10, 100, 1000 and 10000 E. granulosus eggs were prepared and inoculated via intraruminal puncture. Blood samples were obtained before inoculation and every 48 h after inoculation, until they became seropositive. Thereafter, they were taken monthly for the first year and then every three months until 1700 days of observation had been completed. An ELISA test, with total hydatid fluid antigen, was used for immunodiagnosis. The average optical density of the 12 inoculated sheep was found to be above the mean cutoff value 10 days after inoculation, went on increasing until 180 days after inoculation and remained above the cutoff level until the end of the observation period. This confirms that the antibody response of sheep to E. granulosus infection occurs before production of hydatid fluid and that activation, mobilization and establishment of oncospheres in the tissues generates a persistent response from the host's immune system. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000200237 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612014000200237 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612014030 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.23 n.2 2014 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
_version_ |
1754208915237634048 |