Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures

Detalhes bibliográficos
Autor(a) principal: Braga,Fabio Ribeiro
Data de Publicação: 2013
Outros Autores: Araújo,Jackson Victor de, Soares,Filippe Elias de Freitas, Araujo,Juliana Milani, Ferreira,Sebastião Rodrigo, Tavela,Alexandre de Oliveira, Silveira,Wendeo Ferreira da, Queiroz,José Humberto de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100143
Resumo: The objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722) on infective larvae (L3) of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001); group 2: 10 mL of crude extract of AC001 with 10 mM of Ca2+; group 3: 10 mL of crude extract of D. flagrans (CG722); group 4: 10 mL of crude extract of CG722 with 10 mM of Ca2+; and group 5: control group (distilled water). The third-stage larvae (L3) were obtained after eight days. The crude extract of D. flagrans was effective in reducing the number of L3, with the following percentage reductions: group 1, 49.5%; group 2, 52.5%; group 3, 36.8%; and group 4, 57.7%; in relation to the control group (p > 0.05). The proteolytic activity of the crude extract was confirmed through the zymogram. The results from this study confirmed that the crude extract of the fungusD. flagrans could be used for controlling cyathostomin L3, and suggested that at least one protease of approximately 38 kDa was present.
id CBPV-1_6e255143be6f667eaf5688ad076cafe0
oai_identifier_str oai:scielo:S1984-29612013000100143
network_acronym_str CBPV-1
network_name_str Revista Brasileira de Parasitologia Veterinária (Online)
repository_id_str
spelling Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coproculturesNematophagous fungiDuddingtonia flagranszymogramnematodehorsesThe objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722) on infective larvae (L3) of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001); group 2: 10 mL of crude extract of AC001 with 10 mM of Ca2+; group 3: 10 mL of crude extract of D. flagrans (CG722); group 4: 10 mL of crude extract of CG722 with 10 mM of Ca2+; and group 5: control group (distilled water). The third-stage larvae (L3) were obtained after eight days. The crude extract of D. flagrans was effective in reducing the number of L3, with the following percentage reductions: group 1, 49.5%; group 2, 52.5%; group 3, 36.8%; and group 4, 57.7%; in relation to the control group (p > 0.05). The proteolytic activity of the crude extract was confirmed through the zymogram. The results from this study confirmed that the crude extract of the fungusD. flagrans could be used for controlling cyathostomin L3, and suggested that at least one protease of approximately 38 kDa was present.Colégio Brasileiro de Parasitologia Veterinária2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100143Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612013000100026info:eu-repo/semantics/openAccessBraga,Fabio RibeiroAraújo,Jackson Victor deSoares,Filippe Elias de FreitasAraujo,Juliana MilaniFerreira,Sebastião RodrigoTavela,Alexandre de OliveiraSilveira,Wendeo Ferreira daQueiroz,José Humberto deeng2015-11-24T00:00:00Zoai:scielo:S1984-29612013000100143Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-11-24T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
title Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
spellingShingle Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
Braga,Fabio Ribeiro
Nematophagous fungi
Duddingtonia flagrans
zymogram
nematode
horses
title_short Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
title_full Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
title_fullStr Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
title_full_unstemmed Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
title_sort Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae) in coprocultures
author Braga,Fabio Ribeiro
author_facet Braga,Fabio Ribeiro
Araújo,Jackson Victor de
Soares,Filippe Elias de Freitas
Araujo,Juliana Milani
Ferreira,Sebastião Rodrigo
Tavela,Alexandre de Oliveira
Silveira,Wendeo Ferreira da
Queiroz,José Humberto de
author_role author
author2 Araújo,Jackson Victor de
Soares,Filippe Elias de Freitas
Araujo,Juliana Milani
Ferreira,Sebastião Rodrigo
Tavela,Alexandre de Oliveira
Silveira,Wendeo Ferreira da
Queiroz,José Humberto de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Braga,Fabio Ribeiro
Araújo,Jackson Victor de
Soares,Filippe Elias de Freitas
Araujo,Juliana Milani
Ferreira,Sebastião Rodrigo
Tavela,Alexandre de Oliveira
Silveira,Wendeo Ferreira da
Queiroz,José Humberto de
dc.subject.por.fl_str_mv Nematophagous fungi
Duddingtonia flagrans
zymogram
nematode
horses
topic Nematophagous fungi
Duddingtonia flagrans
zymogram
nematode
horses
description The objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722) on infective larvae (L3) of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001); group 2: 10 mL of crude extract of AC001 with 10 mM of Ca2+; group 3: 10 mL of crude extract of D. flagrans (CG722); group 4: 10 mL of crude extract of CG722 with 10 mM of Ca2+; and group 5: control group (distilled water). The third-stage larvae (L3) were obtained after eight days. The crude extract of D. flagrans was effective in reducing the number of L3, with the following percentage reductions: group 1, 49.5%; group 2, 52.5%; group 3, 36.8%; and group 4, 57.7%; in relation to the control group (p > 0.05). The proteolytic activity of the crude extract was confirmed through the zymogram. The results from this study confirmed that the crude extract of the fungusD. flagrans could be used for controlling cyathostomin L3, and suggested that at least one protease of approximately 38 kDa was present.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100143
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100143
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612013000100026
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
_version_ 1754208914500485120