Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages

Detalhes bibliográficos
Autor(a) principal: Espindola,Bruna Dias
Data de Publicação: 2022
Outros Autores: Fernandes,Fagner D’ambroso, Roman,Isac Junior, Samoel,Gisele Vaz Aguirre, Barcelos,Roberto Antônio Delgado, Döhler,Alisson Rodrigues, Botton,Sônia de Ávila, Vogel,Fernanda Silveira Flores, Sangioni,Luis Antonio
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612022000300403
Resumo: Abstract The seroprevalence of Sarcocystis spp. and Toxoplasma gondii was researched in swine raised in Santa Maria, RS, Brazil. Serum samples from 84 pigs from 31 farms were tested using indirect immunofluorescence assay (IFA) for both agents. Additionally, 53 samples of pork sausages and tissues destined for human consumption, including: salami, sausage, black pudding, heart, tongue, brain, and rib muscle, were submitted to PCR to detect DNA for each agent. The frequency of anti-Sarcocystis spp. antibodies was 36.9% (31/84), with titers ranging from 32 to 1024, and 25% (21/84) for anti-T. gondii antibodies, with titers ranging from 64 to 2048. Sarcocystis spp. and T. gondii DNA were detected in 67.9% (36/53) and 13.2% (7/53) of samples, respectively. The presence of antibodies and the detection of DNA from Sarcocystis spp., and T. gondii suggests that the pigs were infected and may serve as an important reservoir for both parasites. The infection by these protozoa in the swine population is relevant to public health due to their zoonotic potential.
id CBPV-1_802b76ecf385069698728ce3acacf535
oai_identifier_str oai:scielo:S1984-29612022000300403
network_acronym_str CBPV-1
network_name_str Revista Brasileira de Parasitologia Veterinária (Online)
repository_id_str
spelling Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausagesSwineSarcocystis spp.Toxoplasma gondiiserologyIFAPCRAbstract The seroprevalence of Sarcocystis spp. and Toxoplasma gondii was researched in swine raised in Santa Maria, RS, Brazil. Serum samples from 84 pigs from 31 farms were tested using indirect immunofluorescence assay (IFA) for both agents. Additionally, 53 samples of pork sausages and tissues destined for human consumption, including: salami, sausage, black pudding, heart, tongue, brain, and rib muscle, were submitted to PCR to detect DNA for each agent. The frequency of anti-Sarcocystis spp. antibodies was 36.9% (31/84), with titers ranging from 32 to 1024, and 25% (21/84) for anti-T. gondii antibodies, with titers ranging from 64 to 2048. Sarcocystis spp. and T. gondii DNA were detected in 67.9% (36/53) and 13.2% (7/53) of samples, respectively. The presence of antibodies and the detection of DNA from Sarcocystis spp., and T. gondii suggests that the pigs were infected and may serve as an important reservoir for both parasites. The infection by these protozoa in the swine population is relevant to public health due to their zoonotic potential.Colégio Brasileiro de Parasitologia Veterinária2022-01-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612022000300403Revista Brasileira de Parasitologia Veterinária v.31 n.3 2022reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612022049info:eu-repo/semantics/openAccessEspindola,Bruna DiasFernandes,Fagner D’ambrosoRoman,Isac JuniorSamoel,Gisele Vaz AguirreBarcelos,Roberto Antônio DelgadoDöhler,Alisson RodriguesBotton,Sônia de ÁvilaVogel,Fernanda Silveira FloresSangioni,Luis Antonioeng2022-08-30T00:00:00Zoai:scielo:S1984-29612022000300403Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2022-08-30T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages
title Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages
spellingShingle Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages
Espindola,Bruna Dias
Swine
Sarcocystis spp.
Toxoplasma gondii
serology
IFA
PCR
title_short Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages
title_full Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages
title_fullStr Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages
title_full_unstemmed Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages
title_sort Detection of Sarcocystis spp. and Toxoplasma gondii in swine and detection of DNA of these protozoa in tissues and sausages
author Espindola,Bruna Dias
author_facet Espindola,Bruna Dias
Fernandes,Fagner D’ambroso
Roman,Isac Junior
Samoel,Gisele Vaz Aguirre
Barcelos,Roberto Antônio Delgado
Döhler,Alisson Rodrigues
Botton,Sônia de Ávila
Vogel,Fernanda Silveira Flores
Sangioni,Luis Antonio
author_role author
author2 Fernandes,Fagner D’ambroso
Roman,Isac Junior
Samoel,Gisele Vaz Aguirre
Barcelos,Roberto Antônio Delgado
Döhler,Alisson Rodrigues
Botton,Sônia de Ávila
Vogel,Fernanda Silveira Flores
Sangioni,Luis Antonio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Espindola,Bruna Dias
Fernandes,Fagner D’ambroso
Roman,Isac Junior
Samoel,Gisele Vaz Aguirre
Barcelos,Roberto Antônio Delgado
Döhler,Alisson Rodrigues
Botton,Sônia de Ávila
Vogel,Fernanda Silveira Flores
Sangioni,Luis Antonio
dc.subject.por.fl_str_mv Swine
Sarcocystis spp.
Toxoplasma gondii
serology
IFA
PCR
topic Swine
Sarcocystis spp.
Toxoplasma gondii
serology
IFA
PCR
description Abstract The seroprevalence of Sarcocystis spp. and Toxoplasma gondii was researched in swine raised in Santa Maria, RS, Brazil. Serum samples from 84 pigs from 31 farms were tested using indirect immunofluorescence assay (IFA) for both agents. Additionally, 53 samples of pork sausages and tissues destined for human consumption, including: salami, sausage, black pudding, heart, tongue, brain, and rib muscle, were submitted to PCR to detect DNA for each agent. The frequency of anti-Sarcocystis spp. antibodies was 36.9% (31/84), with titers ranging from 32 to 1024, and 25% (21/84) for anti-T. gondii antibodies, with titers ranging from 64 to 2048. Sarcocystis spp. and T. gondii DNA were detected in 67.9% (36/53) and 13.2% (7/53) of samples, respectively. The presence of antibodies and the detection of DNA from Sarcocystis spp., and T. gondii suggests that the pigs were infected and may serve as an important reservoir for both parasites. The infection by these protozoa in the swine population is relevant to public health due to their zoonotic potential.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612022000300403
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612022000300403
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1984-29612022049
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.31 n.3 2022
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
_version_ 1754208919380557824