Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil

Detalhes bibliográficos
Autor(a) principal: Ferreira,Tamiris Cristine Ribeiro
Data de Publicação: 2018
Outros Autores: Buery,Julyana Cerqueira, Moreira,Narcisa Imaculada Brant, Santos,Claudiney Biral, Costa,Júlia Gatti Ladeia, Pinto,Lorena Velozo, Baraviera,Ramon Castro de Araújo, Vitor,Ricardo Wagner Almeida, Fux,Blima
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300384
Resumo: Abstract Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molecular aspects of T. gondii allows a better understanding of the epidemiology of toxoplasmosis. This work is a serologic screening of 58 chickens grown (Gallus gallus domesticus) for human consumption in Espírito Santo State, by means of indirect haemagglutination assay (IHA). Thirteen chickens tested positive for anti-T. gondii antibodies. The heart and brain of five positive chickens were harvested, treated with pepsin and inoculated separately, in two Swiss mice, intraperitoneally. Tachyzoites were observed in the peritoneum of all the animals, between seven and 10 days after the inoculum. Ten isolates were obtained and biologically characterised in BALB/c mice inoculated with 101 to 104 tachyzoites. All isolates were classified as virulent or intermediately virulent. Isolates were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, revealing three different genotypes. None of the isolates exhibited the clonal type I, II or III genotype. No genotypic differences were observed between the isolates from the brain or heart from the same bird.
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spelling Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast BrazilToxoplasma gondiiisolationvirulencegenotypingAbstract Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molecular aspects of T. gondii allows a better understanding of the epidemiology of toxoplasmosis. This work is a serologic screening of 58 chickens grown (Gallus gallus domesticus) for human consumption in Espírito Santo State, by means of indirect haemagglutination assay (IHA). Thirteen chickens tested positive for anti-T. gondii antibodies. The heart and brain of five positive chickens were harvested, treated with pepsin and inoculated separately, in two Swiss mice, intraperitoneally. Tachyzoites were observed in the peritoneum of all the animals, between seven and 10 days after the inoculum. Ten isolates were obtained and biologically characterised in BALB/c mice inoculated with 101 to 104 tachyzoites. All isolates were classified as virulent or intermediately virulent. Isolates were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, revealing three different genotypes. None of the isolates exhibited the clonal type I, II or III genotype. No genotypic differences were observed between the isolates from the brain or heart from the same bird.Colégio Brasileiro de Parasitologia Veterinária2018-09-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300384Revista Brasileira de Parasitologia Veterinária v.27 n.3 2018reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-296120180028info:eu-repo/semantics/openAccessFerreira,Tamiris Cristine RibeiroBuery,Julyana CerqueiraMoreira,Narcisa Imaculada BrantSantos,Claudiney BiralCosta,Júlia Gatti LadeiaPinto,Lorena VelozoBaraviera,Ramon Castro de AraújoVitor,Ricardo Wagner AlmeidaFux,Blimaeng2018-09-17T00:00:00Zoai:scielo:S1984-29612018000300384Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2018-09-17T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
title Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
spellingShingle Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
Ferreira,Tamiris Cristine Ribeiro
Toxoplasma gondii
isolation
virulence
genotyping
title_short Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
title_full Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
title_fullStr Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
title_full_unstemmed Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
title_sort Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
author Ferreira,Tamiris Cristine Ribeiro
author_facet Ferreira,Tamiris Cristine Ribeiro
Buery,Julyana Cerqueira
Moreira,Narcisa Imaculada Brant
Santos,Claudiney Biral
Costa,Júlia Gatti Ladeia
Pinto,Lorena Velozo
Baraviera,Ramon Castro de Araújo
Vitor,Ricardo Wagner Almeida
Fux,Blima
author_role author
author2 Buery,Julyana Cerqueira
Moreira,Narcisa Imaculada Brant
Santos,Claudiney Biral
Costa,Júlia Gatti Ladeia
Pinto,Lorena Velozo
Baraviera,Ramon Castro de Araújo
Vitor,Ricardo Wagner Almeida
Fux,Blima
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira,Tamiris Cristine Ribeiro
Buery,Julyana Cerqueira
Moreira,Narcisa Imaculada Brant
Santos,Claudiney Biral
Costa,Júlia Gatti Ladeia
Pinto,Lorena Velozo
Baraviera,Ramon Castro de Araújo
Vitor,Ricardo Wagner Almeida
Fux,Blima
dc.subject.por.fl_str_mv Toxoplasma gondii
isolation
virulence
genotyping
topic Toxoplasma gondii
isolation
virulence
genotyping
description Abstract Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molecular aspects of T. gondii allows a better understanding of the epidemiology of toxoplasmosis. This work is a serologic screening of 58 chickens grown (Gallus gallus domesticus) for human consumption in Espírito Santo State, by means of indirect haemagglutination assay (IHA). Thirteen chickens tested positive for anti-T. gondii antibodies. The heart and brain of five positive chickens were harvested, treated with pepsin and inoculated separately, in two Swiss mice, intraperitoneally. Tachyzoites were observed in the peritoneum of all the animals, between seven and 10 days after the inoculum. Ten isolates were obtained and biologically characterised in BALB/c mice inoculated with 101 to 104 tachyzoites. All isolates were classified as virulent or intermediately virulent. Isolates were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, revealing three different genotypes. None of the isolates exhibited the clonal type I, II or III genotype. No genotypic differences were observed between the isolates from the brain or heart from the same bird.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300384
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/s1984-296120180028
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.27 n.3 2018
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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