Trypanocidal activity of human plasma on Trypanosoma evansi in mice

• Autor(a) principal: Da Silva,Aleksandro Schafer
• Data de Publicação: 2012
• Outros Autores: Duck,Marcos Rafael Kroeker, Fanfa,Vinicius da Rosa, Otto,Mateus Anderson, Nunes,João Tomaz Schmitt, Tonin,Alexandre Alberto, Jaques,Jeandre Augusto, Paim,Francine Chimelo, Duarte,Marta Maria Medeiros Frescura, Monteiro,Silvia Gonzalez
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000100011
• topic: Trypanosoma evansi; treatment; human plasma; APOL1

This study aimed to test an alternative protocol with human plasma to control Trypanosoma evansi infection in mice. Plasma from an apparently 27-year-old healthy male, blood type A+, was used in the study. A concentration of 100 mg.dL-1 apolipoprotein L1 (APOL1) was detected in the plasma. Forty mice were divided into four groups with 10 animals each. Group A comprised uninfected animals. Mice from groups B, C and D were inoculated with a T. evansi isolate. Group B was used as a positive control. At three days post-infection (DPI), the mice were administered intraperitoneally with human plasma. A single dose of 0.2 mL plasma was given to those in group C. The mice from group D were administered five doses of 0.2 mL plasma with a 24 hours interval between the doses. Group B showed high increasing parasitemia that led to their death within 5 DPI. Both treatments eliminated parasites from the blood and increased the longevity of animals. An efficacy of 50 (group C) and 80% (group D) of human plasma trypanocidal activity was found using PCR. This therapeutic success was likely achieved in the group D due to their higher levels of APOL1 compared with group C.