Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil

Detalhes bibliográficos
Autor(a) principal: Fernandes,Marcela Fernanda Torres Samico
Data de Publicação: 2016
Outros Autores: Cavalcanti,Erika Fernanda Torres Samico Fernandes, Silva,José Givanildo da, Mota,André da Rocha, Souza Neto,Orestes Luiz de, Santos,André de Souza, Albuquerque,Pedro Paulo Feitosa de, Lima,Débora Costa Viegas de, Mota,Rinaldo Aparecido
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000100105
Resumo: Abstract The aim of the present study was to investigate the occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chickens bred in the metropolitan area of Recife, Brazil. In total, 212 serum samples were collected from 16 properties, and 12 backyard chickens were collected in the six sanitary districts of Recife. An indirect immunofluorescence assay (IFA) was used to investigate the occurrence of anti-Toxoplasma gondii antibodies. Polymerase chain reaction (PCR) was used to detect T. gondii DNA in brain, heart, liver and lung specimens. Of the samples analyzed by serology, 86/212 (40.56%) were positive; of the samples analyzed by PCR, 2/12 (16.7%) were positive, with both samples positive by both tests (serological and molecular). The presence of antibody anti-T. gondii and parasite DNA in tissues of these animals are worrying aspects for public health because there is a risk of transmission of the parasite to humans through eating undercooked or raw meat. Based on the results, the adoption of preventive measures to prevent the cats access to the chickens creations should be encouraged, since these animals were identified in most of the studied properties.
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spelling Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, BrazilToxoplasmosisGallus gallus domesticusIndirect Immunofluorescence Assay (IFA)Polymerase Chain Reaction (PCR)BrazilAbstract The aim of the present study was to investigate the occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chickens bred in the metropolitan area of Recife, Brazil. In total, 212 serum samples were collected from 16 properties, and 12 backyard chickens were collected in the six sanitary districts of Recife. An indirect immunofluorescence assay (IFA) was used to investigate the occurrence of anti-Toxoplasma gondii antibodies. Polymerase chain reaction (PCR) was used to detect T. gondii DNA in brain, heart, liver and lung specimens. Of the samples analyzed by serology, 86/212 (40.56%) were positive; of the samples analyzed by PCR, 2/12 (16.7%) were positive, with both samples positive by both tests (serological and molecular). The presence of antibody anti-T. gondii and parasite DNA in tissues of these animals are worrying aspects for public health because there is a risk of transmission of the parasite to humans through eating undercooked or raw meat. Based on the results, the adoption of preventive measures to prevent the cats access to the chickens creations should be encouraged, since these animals were identified in most of the studied properties.Colégio Brasileiro de Parasitologia Veterinária2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000100105Revista Brasileira de Parasitologia Veterinária v.25 n.1 2016reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612016012info:eu-repo/semantics/openAccessFernandes,Marcela Fernanda Torres SamicoCavalcanti,Erika Fernanda Torres Samico FernandesSilva,José Givanildo daMota,André da RochaSouza Neto,Orestes Luiz deSantos,André de SouzaAlbuquerque,Pedro Paulo Feitosa deLima,Débora Costa Viegas deMota,Rinaldo Aparecidoeng2016-03-22T00:00:00Zoai:scielo:S1984-29612016000100105Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2016-03-22T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil
title Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil
spellingShingle Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil
Fernandes,Marcela Fernanda Torres Samico
Toxoplasmosis
Gallus gallus domesticus
Indirect Immunofluorescence Assay (IFA)
Polymerase Chain Reaction (PCR)
Brazil
title_short Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil
title_full Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil
title_fullStr Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil
title_full_unstemmed Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil
title_sort Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil
author Fernandes,Marcela Fernanda Torres Samico
author_facet Fernandes,Marcela Fernanda Torres Samico
Cavalcanti,Erika Fernanda Torres Samico Fernandes
Silva,José Givanildo da
Mota,André da Rocha
Souza Neto,Orestes Luiz de
Santos,André de Souza
Albuquerque,Pedro Paulo Feitosa de
Lima,Débora Costa Viegas de
Mota,Rinaldo Aparecido
author_role author
author2 Cavalcanti,Erika Fernanda Torres Samico Fernandes
Silva,José Givanildo da
Mota,André da Rocha
Souza Neto,Orestes Luiz de
Santos,André de Souza
Albuquerque,Pedro Paulo Feitosa de
Lima,Débora Costa Viegas de
Mota,Rinaldo Aparecido
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes,Marcela Fernanda Torres Samico
Cavalcanti,Erika Fernanda Torres Samico Fernandes
Silva,José Givanildo da
Mota,André da Rocha
Souza Neto,Orestes Luiz de
Santos,André de Souza
Albuquerque,Pedro Paulo Feitosa de
Lima,Débora Costa Viegas de
Mota,Rinaldo Aparecido
dc.subject.por.fl_str_mv Toxoplasmosis
Gallus gallus domesticus
Indirect Immunofluorescence Assay (IFA)
Polymerase Chain Reaction (PCR)
Brazil
topic Toxoplasmosis
Gallus gallus domesticus
Indirect Immunofluorescence Assay (IFA)
Polymerase Chain Reaction (PCR)
Brazil
description Abstract The aim of the present study was to investigate the occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chickens bred in the metropolitan area of Recife, Brazil. In total, 212 serum samples were collected from 16 properties, and 12 backyard chickens were collected in the six sanitary districts of Recife. An indirect immunofluorescence assay (IFA) was used to investigate the occurrence of anti-Toxoplasma gondii antibodies. Polymerase chain reaction (PCR) was used to detect T. gondii DNA in brain, heart, liver and lung specimens. Of the samples analyzed by serology, 86/212 (40.56%) were positive; of the samples analyzed by PCR, 2/12 (16.7%) were positive, with both samples positive by both tests (serological and molecular). The presence of antibody anti-T. gondii and parasite DNA in tissues of these animals are worrying aspects for public health because there is a risk of transmission of the parasite to humans through eating undercooked or raw meat. Based on the results, the adoption of preventive measures to prevent the cats access to the chickens creations should be encouraged, since these animals were identified in most of the studied properties.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000100105
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000100105
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612016012
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.25 n.1 2016
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
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repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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