Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300003 |
Resumo: | The importance of dogs as a reservoir for Leishmania infantumchagasi in urban environments has stimulated numerous studies assessing diagnostic techniques. When performed properly, such procedures are an important step in preventing leishmaniasis in humans. Molecular methods have become prominent for this purpose. The aim of the present study was to determine the performance of the polymerase chain reaction (PCR) and real-time PCR (qPCR) for diagnosing of canine visceral leishmaniasis (CVL) using different biological samples. For this, 35 dogs from an area endemic for CVL were used. Bone marrow aspirate and lymph node and spleen fragments from these dogs were used for the molecular diagnosis. In the present study, qPCR was able to detect a greater number of positive animals than seen with PCR. Among the different biological samples used, there was no significant difference in L. infantumchagasi DNA detection between PCR and qPCR. However, considering that lymph nodes are easy to acquire, these can be considered to be the best samples for making molecular diagnoses of L. infantum chagasi infection. |
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Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogsLeishmaniasisdiagnosismolecularL. infantum chagasiThe importance of dogs as a reservoir for Leishmania infantumchagasi in urban environments has stimulated numerous studies assessing diagnostic techniques. When performed properly, such procedures are an important step in preventing leishmaniasis in humans. Molecular methods have become prominent for this purpose. The aim of the present study was to determine the performance of the polymerase chain reaction (PCR) and real-time PCR (qPCR) for diagnosing of canine visceral leishmaniasis (CVL) using different biological samples. For this, 35 dogs from an area endemic for CVL were used. Bone marrow aspirate and lymph node and spleen fragments from these dogs were used for the molecular diagnosis. In the present study, qPCR was able to detect a greater number of positive animals than seen with PCR. Among the different biological samples used, there was no significant difference in L. infantumchagasi DNA detection between PCR and qPCR. However, considering that lymph nodes are easy to acquire, these can be considered to be the best samples for making molecular diagnoses of L. infantum chagasi infection.Colégio Brasileiro de Parasitologia Veterinária2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300003Revista Brasileira de Parasitologia Veterinária v.21 n.3 2012reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612012000300003info:eu-repo/semantics/openAccessRamos,Rafael Antonio do NascimentoRamos,Carlos Alberto do NascimentoJusi,Márcia Mariza GomesAraújo,Flábio Ribeiro deMachado,Rosangela ZacariasFaustino,Maria Aparecida da GlóriaAlves,Leucio Câmaraeng2012-10-11T00:00:00Zoai:scielo:S1984-29612012000300003Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2012-10-11T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs |
title |
Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs |
spellingShingle |
Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs Ramos,Rafael Antonio do Nascimento Leishmaniasis diagnosis molecular L. infantum chagasi |
title_short |
Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs |
title_full |
Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs |
title_fullStr |
Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs |
title_full_unstemmed |
Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs |
title_sort |
Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs |
author |
Ramos,Rafael Antonio do Nascimento |
author_facet |
Ramos,Rafael Antonio do Nascimento Ramos,Carlos Alberto do Nascimento Jusi,Márcia Mariza Gomes Araújo,Flábio Ribeiro de Machado,Rosangela Zacarias Faustino,Maria Aparecida da Glória Alves,Leucio Câmara |
author_role |
author |
author2 |
Ramos,Carlos Alberto do Nascimento Jusi,Márcia Mariza Gomes Araújo,Flábio Ribeiro de Machado,Rosangela Zacarias Faustino,Maria Aparecida da Glória Alves,Leucio Câmara |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ramos,Rafael Antonio do Nascimento Ramos,Carlos Alberto do Nascimento Jusi,Márcia Mariza Gomes Araújo,Flábio Ribeiro de Machado,Rosangela Zacarias Faustino,Maria Aparecida da Glória Alves,Leucio Câmara |
dc.subject.por.fl_str_mv |
Leishmaniasis diagnosis molecular L. infantum chagasi |
topic |
Leishmaniasis diagnosis molecular L. infantum chagasi |
description |
The importance of dogs as a reservoir for Leishmania infantumchagasi in urban environments has stimulated numerous studies assessing diagnostic techniques. When performed properly, such procedures are an important step in preventing leishmaniasis in humans. Molecular methods have become prominent for this purpose. The aim of the present study was to determine the performance of the polymerase chain reaction (PCR) and real-time PCR (qPCR) for diagnosing of canine visceral leishmaniasis (CVL) using different biological samples. For this, 35 dogs from an area endemic for CVL were used. Bone marrow aspirate and lymph node and spleen fragments from these dogs were used for the molecular diagnosis. In the present study, qPCR was able to detect a greater number of positive animals than seen with PCR. Among the different biological samples used, there was no significant difference in L. infantumchagasi DNA detection between PCR and qPCR. However, considering that lymph nodes are easy to acquire, these can be considered to be the best samples for making molecular diagnoses of L. infantum chagasi infection. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612012000300003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.21 n.3 2012 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208914371510272 |