Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100129 |
Resumo: | Bovine anaplasmosis, caused by the tick-borne rickettsiaAnaplasma marginale, is endemic in tropical and subtropical regions of the world and results in economic losses in the cattle industry. Major surface proteins (MSPs) have been used as markers for the genetic characterization of A. marginale strains and demonstrate that many isolates may occur in a given geographic area. However, in Brazil, little is known about the genetic diversity of A. marginale isolates within individual herds. This study was designed to examine the genetic variation among A. marginale infecting calves in a farm in the south of Minas Gerais State, Brazil. Blood samples collected from 100 calves were used to prepare Giemsastained smears that were microscopically examined for the presence of A. marginale. From each blood sample, DNA was extracted and analyzed by a polymerase chain reaction (PCR), followed by sequencing to determine diversity among the isolates. Examination of blood smears showed that 48% of the calves were infected with A. marginale, while the real-time PCR detected 70.2% positivity. Congenital infections were found in four calves. The microsatellite and tandem repeat analyses showed high genetic diversity among the isolates. |
id |
CBPV-1_e46b184b66d8ad5096bde720bbc2cd0b |
---|---|
oai_identifier_str |
oai:scielo:S1984-29612013000100129 |
network_acronym_str |
CBPV-1 |
network_name_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository_id_str |
|
spelling |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, BrazilAnaplasma marginaleMSP1aDNA sequencingmicrosatellitestandem repeatsBrazilBovine anaplasmosis, caused by the tick-borne rickettsiaAnaplasma marginale, is endemic in tropical and subtropical regions of the world and results in economic losses in the cattle industry. Major surface proteins (MSPs) have been used as markers for the genetic characterization of A. marginale strains and demonstrate that many isolates may occur in a given geographic area. However, in Brazil, little is known about the genetic diversity of A. marginale isolates within individual herds. This study was designed to examine the genetic variation among A. marginale infecting calves in a farm in the south of Minas Gerais State, Brazil. Blood samples collected from 100 calves were used to prepare Giemsastained smears that were microscopically examined for the presence of A. marginale. From each blood sample, DNA was extracted and analyzed by a polymerase chain reaction (PCR), followed by sequencing to determine diversity among the isolates. Examination of blood smears showed that 48% of the calves were infected with A. marginale, while the real-time PCR detected 70.2% positivity. Congenital infections were found in four calves. The microsatellite and tandem repeat analyses showed high genetic diversity among the isolates.Colégio Brasileiro de Parasitologia Veterinária2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100129Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612013000100024info:eu-repo/semantics/openAccessPohl,Anna ElisabethCabezas-Cruz,AlejandroRibeiro,Múcio Flávio BarbosaSilveira,Júlia Angélica Gonçalves daSilaghi,CorneliaPfister,KurtPassos,Lygia Maria Fricheeng2015-11-24T00:00:00Zoai:scielo:S1984-29612013000100129Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-11-24T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil |
title |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil |
spellingShingle |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil Pohl,Anna Elisabeth Anaplasma marginale MSP1a DNA sequencing microsatellites tandem repeats Brazil |
title_short |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil |
title_full |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil |
title_fullStr |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil |
title_full_unstemmed |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil |
title_sort |
Detection of genetic diversity of Anaplasma marginale isolates in Minas Gerais, Brazil |
author |
Pohl,Anna Elisabeth |
author_facet |
Pohl,Anna Elisabeth Cabezas-Cruz,Alejandro Ribeiro,Múcio Flávio Barbosa Silveira,Júlia Angélica Gonçalves da Silaghi,Cornelia Pfister,Kurt Passos,Lygia Maria Friche |
author_role |
author |
author2 |
Cabezas-Cruz,Alejandro Ribeiro,Múcio Flávio Barbosa Silveira,Júlia Angélica Gonçalves da Silaghi,Cornelia Pfister,Kurt Passos,Lygia Maria Friche |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pohl,Anna Elisabeth Cabezas-Cruz,Alejandro Ribeiro,Múcio Flávio Barbosa Silveira,Júlia Angélica Gonçalves da Silaghi,Cornelia Pfister,Kurt Passos,Lygia Maria Friche |
dc.subject.por.fl_str_mv |
Anaplasma marginale MSP1a DNA sequencing microsatellites tandem repeats Brazil |
topic |
Anaplasma marginale MSP1a DNA sequencing microsatellites tandem repeats Brazil |
description |
Bovine anaplasmosis, caused by the tick-borne rickettsiaAnaplasma marginale, is endemic in tropical and subtropical regions of the world and results in economic losses in the cattle industry. Major surface proteins (MSPs) have been used as markers for the genetic characterization of A. marginale strains and demonstrate that many isolates may occur in a given geographic area. However, in Brazil, little is known about the genetic diversity of A. marginale isolates within individual herds. This study was designed to examine the genetic variation among A. marginale infecting calves in a farm in the south of Minas Gerais State, Brazil. Blood samples collected from 100 calves were used to prepare Giemsastained smears that were microscopically examined for the presence of A. marginale. From each blood sample, DNA was extracted and analyzed by a polymerase chain reaction (PCR), followed by sequencing to determine diversity among the isolates. Examination of blood smears showed that 48% of the calves were infected with A. marginale, while the real-time PCR detected 70.2% positivity. Congenital infections were found in four calves. The microsatellite and tandem repeat analyses showed high genetic diversity among the isolates. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100129 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100129 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612013000100024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
_version_ |
1754208914497339392 |