Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Detalhes bibliográficos
Autor(a) principal: Lemos,Tatiana Didonet
Data de Publicação: 2012
Outros Autores: Cerqueira,Aloysio de Mello Figueiredo, Toma,Helena Keiko, Silva,Adrianna Vieira da, Corrêa,Rafael Gomes Bartolomeu, Paludo,Giane Regina, Massard,Carlos Luiz, Almosny,Nádia Regina Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200012
Resumo: Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil.
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spelling Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast BrazilBabesia vogeliRangelia vitaliipiroplasmsdogsPCRRangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil.Colégio Brasileiro de Parasitologia Veterinária2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200012Revista Brasileira de Parasitologia Veterinária v.21 n.2 2012reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612012000200012info:eu-repo/semantics/openAccessLemos,Tatiana DidonetCerqueira,Aloysio de Mello FigueiredoToma,Helena KeikoSilva,Adrianna Vieira daCorrêa,Rafael Gomes BartolomeuPaludo,Giane ReginaMassard,Carlos LuizAlmosny,Nádia Regina Pereiraeng2012-07-19T00:00:00Zoai:scielo:S1984-29612012000200012Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2012-07-19T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
title Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
spellingShingle Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
Lemos,Tatiana Didonet
Babesia vogeli
Rangelia vitalii
piroplasms
dogs
PCR
title_short Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
title_full Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
title_fullStr Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
title_full_unstemmed Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
title_sort Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
author Lemos,Tatiana Didonet
author_facet Lemos,Tatiana Didonet
Cerqueira,Aloysio de Mello Figueiredo
Toma,Helena Keiko
Silva,Adrianna Vieira da
Corrêa,Rafael Gomes Bartolomeu
Paludo,Giane Regina
Massard,Carlos Luiz
Almosny,Nádia Regina Pereira
author_role author
author2 Cerqueira,Aloysio de Mello Figueiredo
Toma,Helena Keiko
Silva,Adrianna Vieira da
Corrêa,Rafael Gomes Bartolomeu
Paludo,Giane Regina
Massard,Carlos Luiz
Almosny,Nádia Regina Pereira
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lemos,Tatiana Didonet
Cerqueira,Aloysio de Mello Figueiredo
Toma,Helena Keiko
Silva,Adrianna Vieira da
Corrêa,Rafael Gomes Bartolomeu
Paludo,Giane Regina
Massard,Carlos Luiz
Almosny,Nádia Regina Pereira
dc.subject.por.fl_str_mv Babesia vogeli
Rangelia vitalii
piroplasms
dogs
PCR
topic Babesia vogeli
Rangelia vitalii
piroplasms
dogs
PCR
description Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/S1984-29612012000200012
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.21 n.2 2012
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