Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200012 |
Resumo: | Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil. |
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Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast BrazilBabesia vogeliRangelia vitaliipiroplasmsdogsPCRRangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil.Colégio Brasileiro de Parasitologia Veterinária2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200012Revista Brasileira de Parasitologia Veterinária v.21 n.2 2012reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612012000200012info:eu-repo/semantics/openAccessLemos,Tatiana DidonetCerqueira,Aloysio de Mello FigueiredoToma,Helena KeikoSilva,Adrianna Vieira daCorrêa,Rafael Gomes BartolomeuPaludo,Giane ReginaMassard,Carlos LuizAlmosny,Nádia Regina Pereiraeng2012-07-19T00:00:00Zoai:scielo:S1984-29612012000200012Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2012-07-19T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil |
title |
Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil |
spellingShingle |
Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil Lemos,Tatiana Didonet Babesia vogeli Rangelia vitalii piroplasms dogs PCR |
title_short |
Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil |
title_full |
Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil |
title_fullStr |
Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil |
title_full_unstemmed |
Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil |
title_sort |
Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil |
author |
Lemos,Tatiana Didonet |
author_facet |
Lemos,Tatiana Didonet Cerqueira,Aloysio de Mello Figueiredo Toma,Helena Keiko Silva,Adrianna Vieira da Corrêa,Rafael Gomes Bartolomeu Paludo,Giane Regina Massard,Carlos Luiz Almosny,Nádia Regina Pereira |
author_role |
author |
author2 |
Cerqueira,Aloysio de Mello Figueiredo Toma,Helena Keiko Silva,Adrianna Vieira da Corrêa,Rafael Gomes Bartolomeu Paludo,Giane Regina Massard,Carlos Luiz Almosny,Nádia Regina Pereira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Lemos,Tatiana Didonet Cerqueira,Aloysio de Mello Figueiredo Toma,Helena Keiko Silva,Adrianna Vieira da Corrêa,Rafael Gomes Bartolomeu Paludo,Giane Regina Massard,Carlos Luiz Almosny,Nádia Regina Pereira |
dc.subject.por.fl_str_mv |
Babesia vogeli Rangelia vitalii piroplasms dogs PCR |
topic |
Babesia vogeli Rangelia vitalii piroplasms dogs PCR |
description |
Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000200012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612012000200012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.21 n.2 2012 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208914099929088 |