Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil

Detalhes bibliográficos
Autor(a) principal: Baldani,Cristiane Divan
Data de Publicação: 2010
Outros Autores: Nakaghi,Andrea Cristina Higa, Machado,Rosangela Zacarias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400007
Resumo: Blood and serum samples from 170 horses raised in the Jaboticabal microregion, São Paulo State, Brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR) for Theileria equi infections. The association among the test results was verified by the McNemar test. During the examination of thin blood smears, parasites were detected in six (3.52%) horses. Anti-T. equi antibodies were detected in 100% sera samples, with titers ranging between 1:80 and 1:5120. The nPCR based on the T. equi merozoite antigen gene (EMA-1) allowed the visualization of specie-specific amplified product in 108 (63.53%) horses. All six samples judged positive microscopically were also positive for nPCR. Statistical analysis indicated general disagreement (p < 0.0001) between IFAT and nPCR; IFAT and blood smear; and nPCR and blood smear on the detection of parasite carriers. The results of the present study indicate that T. equi is widely spread among horses in the Jaboticabal microregion, Northeast region of São Paulo State, Brazil.
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spelling Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, BrazilTheileria equihorsediagnosisIFATnested PCRBlood and serum samples from 170 horses raised in the Jaboticabal microregion, São Paulo State, Brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR) for Theileria equi infections. The association among the test results was verified by the McNemar test. During the examination of thin blood smears, parasites were detected in six (3.52%) horses. Anti-T. equi antibodies were detected in 100% sera samples, with titers ranging between 1:80 and 1:5120. The nPCR based on the T. equi merozoite antigen gene (EMA-1) allowed the visualization of specie-specific amplified product in 108 (63.53%) horses. All six samples judged positive microscopically were also positive for nPCR. Statistical analysis indicated general disagreement (p < 0.0001) between IFAT and nPCR; IFAT and blood smear; and nPCR and blood smear on the detection of parasite carriers. The results of the present study indicate that T. equi is widely spread among horses in the Jaboticabal microregion, Northeast region of São Paulo State, Brazil.Colégio Brasileiro de Parasitologia Veterinária2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400007Revista Brasileira de Parasitologia Veterinária v.19 n.4 2010reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612010000400007info:eu-repo/semantics/openAccessBaldani,Cristiane DivanNakaghi,Andrea Cristina HigaMachado,Rosangela Zacariaseng2011-02-07T00:00:00Zoai:scielo:S1984-29612010000400007Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2011-02-07T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
title Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
spellingShingle Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
Baldani,Cristiane Divan
Theileria equi
horse
diagnosis
IFAT
nested PCR
title_short Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
title_full Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
title_fullStr Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
title_full_unstemmed Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
title_sort Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
author Baldani,Cristiane Divan
author_facet Baldani,Cristiane Divan
Nakaghi,Andrea Cristina Higa
Machado,Rosangela Zacarias
author_role author
author2 Nakaghi,Andrea Cristina Higa
Machado,Rosangela Zacarias
author2_role author
author
dc.contributor.author.fl_str_mv Baldani,Cristiane Divan
Nakaghi,Andrea Cristina Higa
Machado,Rosangela Zacarias
dc.subject.por.fl_str_mv Theileria equi
horse
diagnosis
IFAT
nested PCR
topic Theileria equi
horse
diagnosis
IFAT
nested PCR
description Blood and serum samples from 170 horses raised in the Jaboticabal microregion, São Paulo State, Brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR) for Theileria equi infections. The association among the test results was verified by the McNemar test. During the examination of thin blood smears, parasites were detected in six (3.52%) horses. Anti-T. equi antibodies were detected in 100% sera samples, with titers ranging between 1:80 and 1:5120. The nPCR based on the T. equi merozoite antigen gene (EMA-1) allowed the visualization of specie-specific amplified product in 108 (63.53%) horses. All six samples judged positive microscopically were also positive for nPCR. Statistical analysis indicated general disagreement (p < 0.0001) between IFAT and nPCR; IFAT and blood smear; and nPCR and blood smear on the detection of parasite carriers. The results of the present study indicate that T. equi is widely spread among horses in the Jaboticabal microregion, Northeast region of São Paulo State, Brazil.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612010000400007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.19 n.4 2010
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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