Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil

Detalhes bibliográficos
Autor(a) principal: Silva,Bianca Ressetti da
Data de Publicação: 2019
Outros Autores: Ferreira,Morgana de Fátima Kuteques, Maffezzolli,Gabriela, Koch,Marília de Oliveira, Beltrame,Olair Carlos, Taques,Isis Indaiara Gonçalves Granjeiro, Campos,Amanda Noéli da Silva, Aguiar,Daniel Moura de, Dittrich,Rosangela Locatelli
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200310
Resumo: Abstract Rangelia vitalii infects erythrocytes, leukocytes and endothelial cells of dogs. The present study aimed to report the molecular detection confirmed by sequencing of R. vitalii in the state of Paraná, as well as describe the clinical, hematological and biochemical alterations of the infected dogs. Three sick dogs from the metropolitan area of Curitiba, PR, Brazil, underwent a physical exam, and laboratory tests included hematology, biochemistry, polymerase chain reaction (PCR), and gene sequencing. Clinical signs included apathy, anorexia, and hemorrhage. Intra-erythrocytic and extracellular piroplasms were found on peripheral blood smears from all three dogs. Blood samples from these animals were positive for Babesia sp. by PCR targeting 18S rRNA. PCR products from all three dogs were sequenced, and BLAST analysis showed that the PCR-generated sequences were highly homologous with those of R. vitalii previously reported. Hematologic findings included severe anemia, shift of neutrophils to the regenerative left, and thrombocytopenia. Serum urea levels were increased in all three dogs, and direct bilirubin levels were elevated in one dog.
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spelling Detection molecular of Rangelia vitalii in dogs from Parana State, Southern BrazilDoghemoparasitenambiuvúrangeliosistick-borne diseaseAbstract Rangelia vitalii infects erythrocytes, leukocytes and endothelial cells of dogs. The present study aimed to report the molecular detection confirmed by sequencing of R. vitalii in the state of Paraná, as well as describe the clinical, hematological and biochemical alterations of the infected dogs. Three sick dogs from the metropolitan area of Curitiba, PR, Brazil, underwent a physical exam, and laboratory tests included hematology, biochemistry, polymerase chain reaction (PCR), and gene sequencing. Clinical signs included apathy, anorexia, and hemorrhage. Intra-erythrocytic and extracellular piroplasms were found on peripheral blood smears from all three dogs. Blood samples from these animals were positive for Babesia sp. by PCR targeting 18S rRNA. PCR products from all three dogs were sequenced, and BLAST analysis showed that the PCR-generated sequences were highly homologous with those of R. vitalii previously reported. Hematologic findings included severe anemia, shift of neutrophils to the regenerative left, and thrombocytopenia. Serum urea levels were increased in all three dogs, and direct bilirubin levels were elevated in one dog.Colégio Brasileiro de Parasitologia Veterinária2019-04-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200310Revista Brasileira de Parasitologia Veterinária v.28 n.2 2019reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-296120180080info:eu-repo/semantics/openAccessSilva,Bianca Ressetti daFerreira,Morgana de Fátima KutequesMaffezzolli,GabrielaKoch,Marília de OliveiraBeltrame,Olair CarlosTaques,Isis Indaiara Gonçalves GranjeiroCampos,Amanda Noéli da SilvaAguiar,Daniel Moura deDittrich,Rosangela Locatellieng2019-06-18T00:00:00Zoai:scielo:S1984-29612019000200310Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2019-06-18T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
title Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
spellingShingle Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
Silva,Bianca Ressetti da
Dog
hemoparasite
nambiuvú
rangeliosis
tick-borne disease
title_short Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
title_full Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
title_fullStr Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
title_full_unstemmed Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
title_sort Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
author Silva,Bianca Ressetti da
author_facet Silva,Bianca Ressetti da
Ferreira,Morgana de Fátima Kuteques
Maffezzolli,Gabriela
Koch,Marília de Oliveira
Beltrame,Olair Carlos
Taques,Isis Indaiara Gonçalves Granjeiro
Campos,Amanda Noéli da Silva
Aguiar,Daniel Moura de
Dittrich,Rosangela Locatelli
author_role author
author2 Ferreira,Morgana de Fátima Kuteques
Maffezzolli,Gabriela
Koch,Marília de Oliveira
Beltrame,Olair Carlos
Taques,Isis Indaiara Gonçalves Granjeiro
Campos,Amanda Noéli da Silva
Aguiar,Daniel Moura de
Dittrich,Rosangela Locatelli
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Bianca Ressetti da
Ferreira,Morgana de Fátima Kuteques
Maffezzolli,Gabriela
Koch,Marília de Oliveira
Beltrame,Olair Carlos
Taques,Isis Indaiara Gonçalves Granjeiro
Campos,Amanda Noéli da Silva
Aguiar,Daniel Moura de
Dittrich,Rosangela Locatelli
dc.subject.por.fl_str_mv Dog
hemoparasite
nambiuvú
rangeliosis
tick-borne disease
topic Dog
hemoparasite
nambiuvú
rangeliosis
tick-borne disease
description Abstract Rangelia vitalii infects erythrocytes, leukocytes and endothelial cells of dogs. The present study aimed to report the molecular detection confirmed by sequencing of R. vitalii in the state of Paraná, as well as describe the clinical, hematological and biochemical alterations of the infected dogs. Three sick dogs from the metropolitan area of Curitiba, PR, Brazil, underwent a physical exam, and laboratory tests included hematology, biochemistry, polymerase chain reaction (PCR), and gene sequencing. Clinical signs included apathy, anorexia, and hemorrhage. Intra-erythrocytic and extracellular piroplasms were found on peripheral blood smears from all three dogs. Blood samples from these animals were positive for Babesia sp. by PCR targeting 18S rRNA. PCR products from all three dogs were sequenced, and BLAST analysis showed that the PCR-generated sequences were highly homologous with those of R. vitalii previously reported. Hematologic findings included severe anemia, shift of neutrophils to the regenerative left, and thrombocytopenia. Serum urea levels were increased in all three dogs, and direct bilirubin levels were elevated in one dog.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200310
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200310
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1984-296120180080
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.28 n.2 2019
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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