Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200310 |
Resumo: | Abstract Rangelia vitalii infects erythrocytes, leukocytes and endothelial cells of dogs. The present study aimed to report the molecular detection confirmed by sequencing of R. vitalii in the state of Paraná, as well as describe the clinical, hematological and biochemical alterations of the infected dogs. Three sick dogs from the metropolitan area of Curitiba, PR, Brazil, underwent a physical exam, and laboratory tests included hematology, biochemistry, polymerase chain reaction (PCR), and gene sequencing. Clinical signs included apathy, anorexia, and hemorrhage. Intra-erythrocytic and extracellular piroplasms were found on peripheral blood smears from all three dogs. Blood samples from these animals were positive for Babesia sp. by PCR targeting 18S rRNA. PCR products from all three dogs were sequenced, and BLAST analysis showed that the PCR-generated sequences were highly homologous with those of R. vitalii previously reported. Hematologic findings included severe anemia, shift of neutrophils to the regenerative left, and thrombocytopenia. Serum urea levels were increased in all three dogs, and direct bilirubin levels were elevated in one dog. |
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Detection molecular of Rangelia vitalii in dogs from Parana State, Southern BrazilDoghemoparasitenambiuvúrangeliosistick-borne diseaseAbstract Rangelia vitalii infects erythrocytes, leukocytes and endothelial cells of dogs. The present study aimed to report the molecular detection confirmed by sequencing of R. vitalii in the state of Paraná, as well as describe the clinical, hematological and biochemical alterations of the infected dogs. Three sick dogs from the metropolitan area of Curitiba, PR, Brazil, underwent a physical exam, and laboratory tests included hematology, biochemistry, polymerase chain reaction (PCR), and gene sequencing. Clinical signs included apathy, anorexia, and hemorrhage. Intra-erythrocytic and extracellular piroplasms were found on peripheral blood smears from all three dogs. Blood samples from these animals were positive for Babesia sp. by PCR targeting 18S rRNA. PCR products from all three dogs were sequenced, and BLAST analysis showed that the PCR-generated sequences were highly homologous with those of R. vitalii previously reported. Hematologic findings included severe anemia, shift of neutrophils to the regenerative left, and thrombocytopenia. Serum urea levels were increased in all three dogs, and direct bilirubin levels were elevated in one dog.Colégio Brasileiro de Parasitologia Veterinária2019-04-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200310Revista Brasileira de Parasitologia Veterinária v.28 n.2 2019reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-296120180080info:eu-repo/semantics/openAccessSilva,Bianca Ressetti daFerreira,Morgana de Fátima KutequesMaffezzolli,GabrielaKoch,Marília de OliveiraBeltrame,Olair CarlosTaques,Isis Indaiara Gonçalves GranjeiroCampos,Amanda Noéli da SilvaAguiar,Daniel Moura deDittrich,Rosangela Locatellieng2019-06-18T00:00:00Zoai:scielo:S1984-29612019000200310Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2019-06-18T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil |
title |
Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil |
spellingShingle |
Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil Silva,Bianca Ressetti da Dog hemoparasite nambiuvú rangeliosis tick-borne disease |
title_short |
Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil |
title_full |
Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil |
title_fullStr |
Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil |
title_full_unstemmed |
Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil |
title_sort |
Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil |
author |
Silva,Bianca Ressetti da |
author_facet |
Silva,Bianca Ressetti da Ferreira,Morgana de Fátima Kuteques Maffezzolli,Gabriela Koch,Marília de Oliveira Beltrame,Olair Carlos Taques,Isis Indaiara Gonçalves Granjeiro Campos,Amanda Noéli da Silva Aguiar,Daniel Moura de Dittrich,Rosangela Locatelli |
author_role |
author |
author2 |
Ferreira,Morgana de Fátima Kuteques Maffezzolli,Gabriela Koch,Marília de Oliveira Beltrame,Olair Carlos Taques,Isis Indaiara Gonçalves Granjeiro Campos,Amanda Noéli da Silva Aguiar,Daniel Moura de Dittrich,Rosangela Locatelli |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Bianca Ressetti da Ferreira,Morgana de Fátima Kuteques Maffezzolli,Gabriela Koch,Marília de Oliveira Beltrame,Olair Carlos Taques,Isis Indaiara Gonçalves Granjeiro Campos,Amanda Noéli da Silva Aguiar,Daniel Moura de Dittrich,Rosangela Locatelli |
dc.subject.por.fl_str_mv |
Dog hemoparasite nambiuvú rangeliosis tick-borne disease |
topic |
Dog hemoparasite nambiuvú rangeliosis tick-borne disease |
description |
Abstract Rangelia vitalii infects erythrocytes, leukocytes and endothelial cells of dogs. The present study aimed to report the molecular detection confirmed by sequencing of R. vitalii in the state of Paraná, as well as describe the clinical, hematological and biochemical alterations of the infected dogs. Three sick dogs from the metropolitan area of Curitiba, PR, Brazil, underwent a physical exam, and laboratory tests included hematology, biochemistry, polymerase chain reaction (PCR), and gene sequencing. Clinical signs included apathy, anorexia, and hemorrhage. Intra-erythrocytic and extracellular piroplasms were found on peripheral blood smears from all three dogs. Blood samples from these animals were positive for Babesia sp. by PCR targeting 18S rRNA. PCR products from all three dogs were sequenced, and BLAST analysis showed that the PCR-generated sequences were highly homologous with those of R. vitalii previously reported. Hematologic findings included severe anemia, shift of neutrophils to the regenerative left, and thrombocytopenia. Serum urea levels were increased in all three dogs, and direct bilirubin levels were elevated in one dog. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200310 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200310 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-296120180080 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.28 n.2 2019 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208917852782592 |