Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I

Detalhes bibliográficos
Autor(a) principal: Pena,Hilda Fátima de Jesus
Data de Publicação: 2017
Outros Autores: Evangelista,Camila Mariellen, Casagrande,Renata Assis, Biezus,Giovana, Wisser,Claudia Salete, Ferian,Paulo Eduardo, Moura,Anderson Barbosa de, Rolim,Veronica Machado, Driemeier,David, Oliveira,Solange, Alves,Bruna Farias, Gennari,Solange Maria, Traverso,Sandra Davi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177
Resumo: Abstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil.
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spelling Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type IFelis silvestris catusFeLVgenotypingimmunohistochemistrymicrosatellite markersAbstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil.Colégio Brasileiro de Parasitologia Veterinária2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177Revista Brasileira de Parasitologia Veterinária v.26 n.2 2017reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612017025info:eu-repo/semantics/openAccessPena,Hilda Fátima de JesusEvangelista,Camila MariellenCasagrande,Renata AssisBiezus,GiovanaWisser,Claudia SaleteFerian,Paulo EduardoMoura,Anderson Barbosa deRolim,Veronica MachadoDriemeier,DavidOliveira,SolangeAlves,Bruna FariasGennari,Solange MariaTraverso,Sandra Davieng2017-07-12T00:00:00Zoai:scielo:S1984-29612017000200177Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2017-07-12T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
title Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
spellingShingle Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
Pena,Hilda Fátima de Jesus
Felis silvestris catus
FeLV
genotyping
immunohistochemistry
microsatellite markers
title_short Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
title_full Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
title_fullStr Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
title_full_unstemmed Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
title_sort Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
author Pena,Hilda Fátima de Jesus
author_facet Pena,Hilda Fátima de Jesus
Evangelista,Camila Mariellen
Casagrande,Renata Assis
Biezus,Giovana
Wisser,Claudia Salete
Ferian,Paulo Eduardo
Moura,Anderson Barbosa de
Rolim,Veronica Machado
Driemeier,David
Oliveira,Solange
Alves,Bruna Farias
Gennari,Solange Maria
Traverso,Sandra Davi
author_role author
author2 Evangelista,Camila Mariellen
Casagrande,Renata Assis
Biezus,Giovana
Wisser,Claudia Salete
Ferian,Paulo Eduardo
Moura,Anderson Barbosa de
Rolim,Veronica Machado
Driemeier,David
Oliveira,Solange
Alves,Bruna Farias
Gennari,Solange Maria
Traverso,Sandra Davi
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pena,Hilda Fátima de Jesus
Evangelista,Camila Mariellen
Casagrande,Renata Assis
Biezus,Giovana
Wisser,Claudia Salete
Ferian,Paulo Eduardo
Moura,Anderson Barbosa de
Rolim,Veronica Machado
Driemeier,David
Oliveira,Solange
Alves,Bruna Farias
Gennari,Solange Maria
Traverso,Sandra Davi
dc.subject.por.fl_str_mv Felis silvestris catus
FeLV
genotyping
immunohistochemistry
microsatellite markers
topic Felis silvestris catus
FeLV
genotyping
immunohistochemistry
microsatellite markers
description Abstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1984-29612017025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.26 n.2 2017
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
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