Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177 |
Resumo: | Abstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil. |
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Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type IFelis silvestris catusFeLVgenotypingimmunohistochemistrymicrosatellite markersAbstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil.Colégio Brasileiro de Parasitologia Veterinária2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177Revista Brasileira de Parasitologia Veterinária v.26 n.2 2017reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612017025info:eu-repo/semantics/openAccessPena,Hilda Fátima de JesusEvangelista,Camila MariellenCasagrande,Renata AssisBiezus,GiovanaWisser,Claudia SaleteFerian,Paulo EduardoMoura,Anderson Barbosa deRolim,Veronica MachadoDriemeier,DavidOliveira,SolangeAlves,Bruna FariasGennari,Solange MariaTraverso,Sandra Davieng2017-07-12T00:00:00Zoai:scielo:S1984-29612017000200177Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2017-07-12T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
spellingShingle |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I Pena,Hilda Fátima de Jesus Felis silvestris catus FeLV genotyping immunohistochemistry microsatellite markers |
title_short |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_full |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_fullStr |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_full_unstemmed |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
title_sort |
Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I |
author |
Pena,Hilda Fátima de Jesus |
author_facet |
Pena,Hilda Fátima de Jesus Evangelista,Camila Mariellen Casagrande,Renata Assis Biezus,Giovana Wisser,Claudia Salete Ferian,Paulo Eduardo Moura,Anderson Barbosa de Rolim,Veronica Machado Driemeier,David Oliveira,Solange Alves,Bruna Farias Gennari,Solange Maria Traverso,Sandra Davi |
author_role |
author |
author2 |
Evangelista,Camila Mariellen Casagrande,Renata Assis Biezus,Giovana Wisser,Claudia Salete Ferian,Paulo Eduardo Moura,Anderson Barbosa de Rolim,Veronica Machado Driemeier,David Oliveira,Solange Alves,Bruna Farias Gennari,Solange Maria Traverso,Sandra Davi |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pena,Hilda Fátima de Jesus Evangelista,Camila Mariellen Casagrande,Renata Assis Biezus,Giovana Wisser,Claudia Salete Ferian,Paulo Eduardo Moura,Anderson Barbosa de Rolim,Veronica Machado Driemeier,David Oliveira,Solange Alves,Bruna Farias Gennari,Solange Maria Traverso,Sandra Davi |
dc.subject.por.fl_str_mv |
Felis silvestris catus FeLV genotyping immunohistochemistry microsatellite markers |
topic |
Felis silvestris catus FeLV genotyping immunohistochemistry microsatellite markers |
description |
Abstract The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000200177 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-29612017025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.26 n.2 2017 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208916639580160 |