Luting agents differentially modulate inflammation and matrix metalloproteinases in connective tissue

• Autor(a) principal: de Queiroz, Alexandra Mussolino
• Data de Publicação: 2018
• Outros Autores: do Amaral, Thais Helena Andreolli, Paula-Silva, Francisco Wanderley Garcia, Filho, Paulo Nelson, Bezerra da Silva, Raquel Assed, Maria Gaton-Hernández, Patrícia, Assed Bezerra da Silva, Léa
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Científica do CRO-RJ (Online)
• Texto Completo: https://cro-rj.org.br/revcientifica/index.php/revista/article/view/15
• topic: luting agents; resin cements; glass ionomer cement; connective tissue response; matrix metalloproteinases; agentes cimentantes; cimentos resinosos; cimentos ionoméricos; resposta do tecido conjuntivo; metaloproteinases da matriz

Aim: The objective of this study was to evaluate the tissue response and expression of matrix metalloproteinases (MMP)-2 and -9 to RelyXTM Unicem (RU) and KetacTM Cem Easymix (KC) cements in direct contact with the subcutaneous connective tissue. Methods: RU (n=30), KC (n=30), and empty tubes (control; n=30) were implanted in the dorsal subcutaneous tissue of isogenic BALB/c mice, and the tissues were biopsied after 7, 21, and 63 days for histological analysis. The inflammatory cells and fibroblasts were counted, and the fibrous capsule thickness was measured. MMP-2 and MMP-9 expression levels were investigated by immunohistochemistry. Data were analyzed statistically (significance level=5%). Results: We found that RU induced a low inflammation at day 7 and 21, which was increased at day 63 (p<0.05). KC induced a more intense mononuclear inflammatory response at day 7 and 21 (p<0.05), which was reduced at day 63 to levels similar to the control (p>0.05). The fibrous capsule thickness was thin for RU, KC, and control (p>0.05). MMP-2 was detected early for KC and RU and decreased afterwards. MMP-9 presented a similar pattern for KC, whereas the MMP-9 expression was late for RU. Conclusion: RU induced an inflammatory response and late MMP-9 expression in the subcutaneous connective tissue that was different from that induced by KC.