Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice

Detalhes bibliográficos
Autor(a) principal: Coutinho, Diego de Sá
Data de Publicação: 2020
Outros Autores: Pires, Jader, Gomes, Hyago, Pohlmann, Adriana Raffin, Guterres, Sílvia Stanisçuaski, Silva, Patrícia Machado Rodrigues e, Martins, Marco Aurélio, Ferrarini, Stela Regina, Bernardi, Andressa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45988
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
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spelling Coutinho, Diego de SáPires, JaderGomes, HyagoPohlmann, Adriana RaffinGuterres, Sílvia StanisçuaskiSilva, Patrícia Machado Rodrigues eMartins, Marco AurélioFerrarini, Stela ReginaBernardi, Andressa2021-02-08T14:08:12Z2021-02-08T14:08:12Z2020COUTINHO, Diego de Sá et al. Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice. Pharmaceutics, v. 12, n.1075, 12p, 2020.1999-4923https://www.arca.fiocruz.br/handle/icict/4598810.3390/pharmaceutics12111075engMDPILesão pulmonar aguda (ALI)/respiratória agudaNanoemulsãoEntrega de drogasÁcido oleicoÓleo de PequiAcute lung injury (ALI)/acute respiratory distress syndrome (ARDS);NanoemulsionDrug deliveryPequi oilOleic acidPequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Instituto de Química. Departamento de Química Orgânica. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Escola de Farmácia. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Escola de Farmácia. Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Pequi is a Brazilian fruit used in folk medicine for pulmonary diseases treatment, but its oil presents bioavailability limitations. The use of nanocarriers can overcome this limitation. We developed nanoemulsions containing pequi oil (pequi-NE) and evaluated their e ects in a lipopolysaccharide (LPS)-induced lung injury model. Free pequi oil or pequi-NE (20 mg/kg) was orally administered to A/J mice 16 and 4 h prior to intranasal LPS exposure, and the analyses were performed 24 h after LPS provocation. The physicochemical results revealed that pequi-NE comprised particles with mean diameter of 174–223 nm, low polydispersity index (0.11 0.01), zeta potential of 7.13 0.08 mV, and pH of 5.83 0.12. In vivo evaluation showed that free pequi oil pretreatment reduced the influx of inflammatory cells into bronchoalveolar fluid (BALF), while pequi-NE completely abolished leukocyte accumulation. Moreover, pequi-NE, but not free pequi oil, reduced myeloperoxidase (MPO), TNF- , IL-1 , IL-6, MCP-1, and KC levels. Similar anti-inflammatory e ects were observed when LPS-exposed animals were pre-treated with the nanoemulsion containing pequi or oleic acid. These results suggest that the use of nanoemulsions as carriers enhances the anti-inflammatory properties of oleic acid-containing pequi oil. Moreover, pequi’s beneficial e ect is likely due its high levels of oleic acid.Pequi is a Brazilian fruit used in folk medicine for pulmonary diseases treatment, but its oil presents bioavailability limitations. The use of nanocarriers can overcome this limitation. We developed nanoemulsions containing pequi oil (pequi-NE) and evaluated their e ects in a lipopolysaccharide (LPS)-induced lung injury model. Free pequi oil or pequi-NE (20 mg/kg) was orally administered to A/J mice 16 and 4 h prior to intranasal LPS exposure, and the analyses were performed 24 h after LPS provocation. The physicochemical results revealed that pequi-NE comprised particles with mean diameter of 174–223 nm, low polydispersity index (0.11 0.01), zeta potential of 7.13 0.08 mV, and pH of 5.83 0.12. In vivo evaluation showed that free pequi oil pretreatment reduced the influx of inflammatory cells into bronchoalveolar fluid (BALF), while pequi-NE completely abolished leukocyte accumulation. Moreover, pequi-NE, but not free pequi oil, reduced myeloperoxidase (MPO), TNF- , IL-1 , IL-6, MCP-1, and KC levels. Similar anti-inflammatory e ects were observed when LPS-exposed animals were pre-treated with the nanoemulsion containing pequi or oleic acid. These results suggest that the use of nanoemulsions as carriers enhances the anti-inflammatory properties of oleic acid-containing pequi oil. Moreover, pequi’s beneficial e ect is likely due its high levels of oleic acid.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45988/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDiegoCoutinho_AndressaBernardi_etal_IOC_2020.pdfDiegoCoutinho_AndressaBernardi_etal_IOC_2020.pdfapplication/pdf2566008https://www.arca.fiocruz.br/bitstream/icict/45988/2/DiegoCoutinho_AndressaBernardi_etal_IOC_2020.pdf9c1bdfef686311f327247cbbc9f67f85MD52TEXTDiegoCoutinho_AndressaBernardi_etal_IOC_2020.pdf.txtDiegoCoutinho_AndressaBernardi_etal_IOC_2020.pdf.txtExtracted texttext/plain78147https://www.arca.fiocruz.br/bitstream/icict/45988/3/DiegoCoutinho_AndressaBernardi_etal_IOC_2020.pdf.txt75f4e4a3b8c20ea2ad3428f4b42365bbMD53icict/459882021-02-09 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dc.title.pt_BR.fl_str_mv Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice
title Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice
spellingShingle Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice
Coutinho, Diego de Sá
Lesão pulmonar aguda (ALI)/respiratória aguda
Nanoemulsão
Entrega de drogas
Ácido oleico
Óleo de Pequi
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS);
Nanoemulsion
Drug delivery
Pequi oil
Oleic acid
title_short Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice
title_full Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice
title_fullStr Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice
title_full_unstemmed Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice
title_sort Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice
author Coutinho, Diego de Sá
author_facet Coutinho, Diego de Sá
Pires, Jader
Gomes, Hyago
Pohlmann, Adriana Raffin
Guterres, Sílvia Stanisçuaski
Silva, Patrícia Machado Rodrigues e
Martins, Marco Aurélio
Ferrarini, Stela Regina
Bernardi, Andressa
author_role author
author2 Pires, Jader
Gomes, Hyago
Pohlmann, Adriana Raffin
Guterres, Sílvia Stanisçuaski
Silva, Patrícia Machado Rodrigues e
Martins, Marco Aurélio
Ferrarini, Stela Regina
Bernardi, Andressa
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Coutinho, Diego de Sá
Pires, Jader
Gomes, Hyago
Pohlmann, Adriana Raffin
Guterres, Sílvia Stanisçuaski
Silva, Patrícia Machado Rodrigues e
Martins, Marco Aurélio
Ferrarini, Stela Regina
Bernardi, Andressa
dc.subject.other.pt_BR.fl_str_mv Lesão pulmonar aguda (ALI)/respiratória aguda
Nanoemulsão
Entrega de drogas
Ácido oleico
Óleo de Pequi
topic Lesão pulmonar aguda (ALI)/respiratória aguda
Nanoemulsão
Entrega de drogas
Ácido oleico
Óleo de Pequi
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS);
Nanoemulsion
Drug delivery
Pequi oil
Oleic acid
dc.subject.en.pt_BR.fl_str_mv Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS);
Nanoemulsion
Drug delivery
Pequi oil
Oleic acid
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-02-08T14:08:12Z
dc.date.available.fl_str_mv 2021-02-08T14:08:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COUTINHO, Diego de Sá et al. Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice. Pharmaceutics, v. 12, n.1075, 12p, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45988
dc.identifier.issn.pt_BR.fl_str_mv 1999-4923
dc.identifier.doi.none.fl_str_mv 10.3390/pharmaceutics12111075
identifier_str_mv COUTINHO, Diego de Sá et al. Pequi (Caryocar brasiliense Cambess)-Loaded Nanoemulsion, Orally Delivered, Modulates Inflammation in LPS-Induced Acute Lung Injury in Mice. Pharmaceutics, v. 12, n.1075, 12p, 2020.
1999-4923
10.3390/pharmaceutics12111075
url https://www.arca.fiocruz.br/handle/icict/45988
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/45988/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/45988/2/DiegoCoutinho_AndressaBernardi_etal_IOC_2020.pdf
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