Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line

Detalhes bibliográficos
Autor(a) principal: Siqueira, Elaine da Silva
Data de Publicação: 2020
Outros Autores: Concato, Virgínia Márcia, Tomiotto-Pellissier, Fernanda, Silva, Taylon Felipe, Bortoleti, Bruna Taciane da Silva, Gonçalves, Manoela Daiele, Costa, Idessania Nazareth, Verri Junior, Waldiceu Aparecido, Pavanelli, Wander Rogério, Panis, Carolina, Mantovani, Mário Sérgio, Miranda-Sapla, Milena Menegazzo, Conchon-Costa, Ivete
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44533
Resumo: Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.
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spelling Siqueira, Elaine da SilvaConcato, Virgínia MárciaTomiotto-Pellissier, FernandaSilva, Taylon FelipeBortoleti, Bruna Taciane da SilvaGonçalves, Manoela DaieleCosta, Idessania NazarethVerri Junior, Waldiceu AparecidoPavanelli, Wander RogérioPanis, CarolinaMantovani, Mário SérgioMiranda-Sapla, Milena MenegazzoConchon-Costa, Ivete2020-11-18T23:31:01Z2020-11-18T23:31:01Z2020SIQUEIRA, Elaine da Silva et al. Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line. Phytomedicine. v. 80, n. 153373, p. 1-9, 2020.0944-7113https://www.arca.fiocruz.br/handle/icict/4453310.1016/j.phymed.2020.153373porElsevierTrans-ChalconesCarcinoma, HepatocellularChalconesAutophagyTumor Suppressor Protein p53beta CateninCarcinoma HepatocelularChalconasAutofagiaProteína p53 Supresora de TumorCarcinome hépatocellulaireAutophagieProtéine p53 suppresseur de tumeurbêta-CaténineCarcinoma HepatocelularChalconasAutofagiaProteína Supressora de Tumor p53beta CateninaTrans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell lineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-Graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-Graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Biotransformação e Fitoquímica. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Dor, Inflamação e Neuropatia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual do Oeste do Paraná. Laboratório de Biologia de Tumores. Francisco Beltrão, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Genética Toxicológica. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Hepatocellular Carcinoma (HCC) is extremely aggressive and presents low rates of response to the available chemotherapeutic agents. Many studies have focused on the search for alternative low-cost natural compounds with antiproliferative potential that selectively respond to liver cancer cells. This study assessed the in vitro direct action of trans-chalcone (TC) on cells of the human HCC HuH7.5 cell line. As methods, we subjected the HuH7.5 tumor cells to TC treatment at increasing concentrations (12.5–100 µM) for 24 and 48 h. Cell viability was verified through MTT and 50% inhibitory concentration of cells (IC50 23.66 µM) was determined within 48 h. We quantified trypan blue proliferation and light microscopy, ROS production, mitochondrial depolarization and autophagy, cell cycle analysis, and apoptosis using Muse® cell analyzer and immunocytochemical markings of p53 and β-catenin. In the results, data showed an effective dose- and time-dependent TC-cytotoxic action at low micromolar concentrations without causing toxicity to non-cancerous cells, such as erythrocytes. TC-treatment caused mitochondrial membrane damage and cell cycle G0/G1 phase arrest, increasing the presence of the p53 protein and decreasing β-catenin, in addition, to inducing cell death by autophagy. Additionally, TC decreased the metastatic capacity of HuH7.5, which affected the migration/invasion of this type of cell. We had as conclusion that: In vitro TC activity in the human HCC HuH7.5 tumor cell line is shown to be a potential molecule to develop new therapies to repair the p53 pathway and prevent the overexpression of Wnt/β-catenin tumor development inducing autophagy cell death and decreasing metastatic capacity of HuH7.5 cell line.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/44533/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALS094471132030204XOK.pdfS094471132030204XOK.pdfapplication/pdf5206269https://www.arca.fiocruz.br/bitstream/icict/44533/2/S094471132030204XOK.pdfa9f91df2c92354e7e49010548923d6e3MD52TEXTS094471132030204XOK.pdf.txtS094471132030204XOK.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
title Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
spellingShingle Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
Siqueira, Elaine da Silva
Trans-Chalcones
Carcinoma, Hepatocellular
Chalcones
Autophagy
Tumor Suppressor Protein p53
beta Catenin
Carcinoma Hepatocelular
Chalconas
Autofagia
Proteína p53 Supresora de Tumor
Carcinome hépatocellulaire
Autophagie
Protéine p53 suppresseur de tumeur
bêta-Caténine
Carcinoma Hepatocelular
Chalconas
Autofagia
Proteína Supressora de Tumor p53
beta Catenina
title_short Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
title_full Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
title_fullStr Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
title_full_unstemmed Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
title_sort Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
author Siqueira, Elaine da Silva
author_facet Siqueira, Elaine da Silva
Concato, Virgínia Márcia
Tomiotto-Pellissier, Fernanda
Silva, Taylon Felipe
Bortoleti, Bruna Taciane da Silva
Gonçalves, Manoela Daiele
Costa, Idessania Nazareth
Verri Junior, Waldiceu Aparecido
Pavanelli, Wander Rogério
Panis, Carolina
Mantovani, Mário Sérgio
Miranda-Sapla, Milena Menegazzo
Conchon-Costa, Ivete
author_role author
author2 Concato, Virgínia Márcia
Tomiotto-Pellissier, Fernanda
Silva, Taylon Felipe
Bortoleti, Bruna Taciane da Silva
Gonçalves, Manoela Daiele
Costa, Idessania Nazareth
Verri Junior, Waldiceu Aparecido
Pavanelli, Wander Rogério
Panis, Carolina
Mantovani, Mário Sérgio
Miranda-Sapla, Milena Menegazzo
Conchon-Costa, Ivete
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Siqueira, Elaine da Silva
Concato, Virgínia Márcia
Tomiotto-Pellissier, Fernanda
Silva, Taylon Felipe
Bortoleti, Bruna Taciane da Silva
Gonçalves, Manoela Daiele
Costa, Idessania Nazareth
Verri Junior, Waldiceu Aparecido
Pavanelli, Wander Rogério
Panis, Carolina
Mantovani, Mário Sérgio
Miranda-Sapla, Milena Menegazzo
Conchon-Costa, Ivete
dc.subject.other.pt_BR.fl_str_mv Trans-Chalcones
topic Trans-Chalcones
Carcinoma, Hepatocellular
Chalcones
Autophagy
Tumor Suppressor Protein p53
beta Catenin
Carcinoma Hepatocelular
Chalconas
Autofagia
Proteína p53 Supresora de Tumor
Carcinome hépatocellulaire
Autophagie
Protéine p53 suppresseur de tumeur
bêta-Caténine
Carcinoma Hepatocelular
Chalconas
Autofagia
Proteína Supressora de Tumor p53
beta Catenina
dc.subject.en.pt_BR.fl_str_mv Carcinoma, Hepatocellular
Chalcones
Autophagy
Tumor Suppressor Protein p53
beta Catenin
dc.subject.es.pt_BR.fl_str_mv Carcinoma Hepatocelular
Chalconas
Autofagia
Proteína p53 Supresora de Tumor
dc.subject.fr.pt_BR.fl_str_mv Carcinome hépatocellulaire
Autophagie
Protéine p53 suppresseur de tumeur
bêta-Caténine
dc.subject.decs.pt_BR.fl_str_mv Carcinoma Hepatocelular
Chalconas
Autofagia
Proteína Supressora de Tumor p53
beta Catenina
description Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-11-18T23:31:01Z
dc.date.available.fl_str_mv 2020-11-18T23:31:01Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SIQUEIRA, Elaine da Silva et al. Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line. Phytomedicine. v. 80, n. 153373, p. 1-9, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44533
dc.identifier.issn.pt_BR.fl_str_mv 0944-7113
dc.identifier.doi.none.fl_str_mv 10.1016/j.phymed.2020.153373
identifier_str_mv SIQUEIRA, Elaine da Silva et al. Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line. Phytomedicine. v. 80, n. 153373, p. 1-9, 2020.
0944-7113
10.1016/j.phymed.2020.153373
url https://www.arca.fiocruz.br/handle/icict/44533
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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