Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44533 |
Resumo: | Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. |
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Siqueira, Elaine da SilvaConcato, Virgínia MárciaTomiotto-Pellissier, FernandaSilva, Taylon FelipeBortoleti, Bruna Taciane da SilvaGonçalves, Manoela DaieleCosta, Idessania NazarethVerri Junior, Waldiceu AparecidoPavanelli, Wander RogérioPanis, CarolinaMantovani, Mário SérgioMiranda-Sapla, Milena MenegazzoConchon-Costa, Ivete2020-11-18T23:31:01Z2020-11-18T23:31:01Z2020SIQUEIRA, Elaine da Silva et al. Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line. Phytomedicine. v. 80, n. 153373, p. 1-9, 2020.0944-7113https://www.arca.fiocruz.br/handle/icict/4453310.1016/j.phymed.2020.153373porElsevierTrans-ChalconesCarcinoma, HepatocellularChalconesAutophagyTumor Suppressor Protein p53beta CateninCarcinoma HepatocelularChalconasAutofagiaProteína p53 Supresora de TumorCarcinome hépatocellulaireAutophagieProtéine p53 suppresseur de tumeurbêta-CaténineCarcinoma HepatocelularChalconasAutofagiaProteína Supressora de Tumor p53beta CateninaTrans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell lineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-Graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-Graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Biotransformação e Fitoquímica. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Dor, Inflamação e Neuropatia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual do Oeste do Paraná. Laboratório de Biologia de Tumores. Francisco Beltrão, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Genética Toxicológica. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Hepatocellular Carcinoma (HCC) is extremely aggressive and presents low rates of response to the available chemotherapeutic agents. Many studies have focused on the search for alternative low-cost natural compounds with antiproliferative potential that selectively respond to liver cancer cells. This study assessed the in vitro direct action of trans-chalcone (TC) on cells of the human HCC HuH7.5 cell line. As methods, we subjected the HuH7.5 tumor cells to TC treatment at increasing concentrations (12.5–100 µM) for 24 and 48 h. Cell viability was verified through MTT and 50% inhibitory concentration of cells (IC50 23.66 µM) was determined within 48 h. We quantified trypan blue proliferation and light microscopy, ROS production, mitochondrial depolarization and autophagy, cell cycle analysis, and apoptosis using Muse® cell analyzer and immunocytochemical markings of p53 and β-catenin. In the results, data showed an effective dose- and time-dependent TC-cytotoxic action at low micromolar concentrations without causing toxicity to non-cancerous cells, such as erythrocytes. TC-treatment caused mitochondrial membrane damage and cell cycle G0/G1 phase arrest, increasing the presence of the p53 protein and decreasing β-catenin, in addition, to inducing cell death by autophagy. Additionally, TC decreased the metastatic capacity of HuH7.5, which affected the migration/invasion of this type of cell. We had as conclusion that: In vitro TC activity in the human HCC HuH7.5 tumor cell line is shown to be a potential molecule to develop new therapies to repair the p53 pathway and prevent the overexpression of Wnt/β-catenin tumor development inducing autophagy cell death and decreasing metastatic capacity of HuH7.5 cell line.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/44533/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALS094471132030204XOK.pdfS094471132030204XOK.pdfapplication/pdf5206269https://www.arca.fiocruz.br/bitstream/icict/44533/2/S094471132030204XOK.pdfa9f91df2c92354e7e49010548923d6e3MD52TEXTS094471132030204XOK.pdf.txtS094471132030204XOK.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line |
| title |
Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line |
| spellingShingle |
Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line Siqueira, Elaine da Silva Trans-Chalcones Carcinoma, Hepatocellular Chalcones Autophagy Tumor Suppressor Protein p53 beta Catenin Carcinoma Hepatocelular Chalconas Autofagia Proteína p53 Supresora de Tumor Carcinome hépatocellulaire Autophagie Protéine p53 suppresseur de tumeur bêta-Caténine Carcinoma Hepatocelular Chalconas Autofagia Proteína Supressora de Tumor p53 beta Catenina |
| title_short |
Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line |
| title_full |
Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line |
| title_fullStr |
Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line |
| title_full_unstemmed |
Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line |
| title_sort |
Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line |
| author |
Siqueira, Elaine da Silva |
| author_facet |
Siqueira, Elaine da Silva Concato, Virgínia Márcia Tomiotto-Pellissier, Fernanda Silva, Taylon Felipe Bortoleti, Bruna Taciane da Silva Gonçalves, Manoela Daiele Costa, Idessania Nazareth Verri Junior, Waldiceu Aparecido Pavanelli, Wander Rogério Panis, Carolina Mantovani, Mário Sérgio Miranda-Sapla, Milena Menegazzo Conchon-Costa, Ivete |
| author_role |
author |
| author2 |
Concato, Virgínia Márcia Tomiotto-Pellissier, Fernanda Silva, Taylon Felipe Bortoleti, Bruna Taciane da Silva Gonçalves, Manoela Daiele Costa, Idessania Nazareth Verri Junior, Waldiceu Aparecido Pavanelli, Wander Rogério Panis, Carolina Mantovani, Mário Sérgio Miranda-Sapla, Milena Menegazzo Conchon-Costa, Ivete |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Siqueira, Elaine da Silva Concato, Virgínia Márcia Tomiotto-Pellissier, Fernanda Silva, Taylon Felipe Bortoleti, Bruna Taciane da Silva Gonçalves, Manoela Daiele Costa, Idessania Nazareth Verri Junior, Waldiceu Aparecido Pavanelli, Wander Rogério Panis, Carolina Mantovani, Mário Sérgio Miranda-Sapla, Milena Menegazzo Conchon-Costa, Ivete |
| dc.subject.other.pt_BR.fl_str_mv |
Trans-Chalcones |
| topic |
Trans-Chalcones Carcinoma, Hepatocellular Chalcones Autophagy Tumor Suppressor Protein p53 beta Catenin Carcinoma Hepatocelular Chalconas Autofagia Proteína p53 Supresora de Tumor Carcinome hépatocellulaire Autophagie Protéine p53 suppresseur de tumeur bêta-Caténine Carcinoma Hepatocelular Chalconas Autofagia Proteína Supressora de Tumor p53 beta Catenina |
| dc.subject.en.pt_BR.fl_str_mv |
Carcinoma, Hepatocellular Chalcones Autophagy Tumor Suppressor Protein p53 beta Catenin |
| dc.subject.es.pt_BR.fl_str_mv |
Carcinoma Hepatocelular Chalconas Autofagia Proteína p53 Supresora de Tumor |
| dc.subject.fr.pt_BR.fl_str_mv |
Carcinome hépatocellulaire Autophagie Protéine p53 suppresseur de tumeur bêta-Caténine |
| dc.subject.decs.pt_BR.fl_str_mv |
Carcinoma Hepatocelular Chalconas Autofagia Proteína Supressora de Tumor p53 beta Catenina |
| description |
Universidade Estadual de Londrina. Laboratório de Imunopatologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. |
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2020 |
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2020-11-18T23:31:01Z |
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2020-11-18T23:31:01Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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| dc.identifier.citation.fl_str_mv |
SIQUEIRA, Elaine da Silva et al. Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line. Phytomedicine. v. 80, n. 153373, p. 1-9, 2020. |
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https://www.arca.fiocruz.br/handle/icict/44533 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0944-7113 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.phymed.2020.153373 |
| identifier_str_mv |
SIQUEIRA, Elaine da Silva et al. Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line. Phytomedicine. v. 80, n. 153373, p. 1-9, 2020. 0944-7113 10.1016/j.phymed.2020.153373 |
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Elsevier |
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Elsevier |
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