Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis

Detalhes bibliográficos
Autor(a) principal: Vieira, Alexandre Zanatta
Data de Publicação: 2021
Outros Autores: Raittz, Roberto Tadeu, Faoro, Helisson
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/47138
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil / Universidade Federal do Paraná. Programa de Graduação em Bioinformática. Curitiba, PR, Brasil.
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spelling Vieira, Alexandre ZanattaRaittz, Roberto TadeuFaoro, Helisson2021-05-11T19:34:01Z2021-05-11T19:34:01Z2021VIEIRA, Alexandre Zanatta; RAITTZ, Roberto Tadeu; FAORO, Helisson. Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis. Microbial Genomics, v.7, n. 563, p. 1-14, 2021.2057-5858https://www.arca.fiocruz.br/handle/icict/4713810.1099/mgen.0.000563porMicrobiology SocietyOrigin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil / Universidade Federal do Paraná. Programa de Graduação em Bioinformática. Curitiba, PR, Brasil.Universidade Federal do Paraná. Programa de Graduação em Bioinformática. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil / Universidade Federal do Paraná. Programa de Graduação em Bioinformática. Curitiba, PR, Brasil.Nonulosonic acids (NulOs) are a group of nine-carbon monosaccharides with different functions in nature. N-acetylneuraminic acid (Neu5Ac) is the most common NulO. It covers the membrane surface of all human cells and is a central molecule in the process of self-recognition via SIGLECS receptors. Some pathogenic bacteria escape the immune system by copying the sialylation of the host cell membrane. Neu5Ac production in these bacteria is catalysed by the enzyme NeuB. Some bacteria can also produce other NulOs named pseudaminic and legionaminic acids, through the NeuB homologues PseI and LegI, respectively. In Opisthokonta eukaryotes, the biosynthesis of Neu5Ac is catalysed by the enzyme NanS. In this study, we used publicly available data of sequences of NulOs synthases to investigate its distribution within the three domains of life and its relationship with pathogenic bacteria. We mined the KEGG database and found 425 NeuB sequences. Most NeuB sequences (58.74%) from the KEGG orthology database were classified as from environmental bacteria; however, sequences from pathogenic bacteria showed higher conservation and prevalence of a specific domain named SAF. Using the HMM profile we identified 13941 NulO synthase sequences in UniProt. Phylogenetic analysis of these sequences showed that the synthases were divided into three main groups that can be related to the lifestyle of these bacteria: (I) predominantly environmental, (II) intermediate and (III) predominantly pathogenic. NeuB was widely distributed in the groups. However, LegI and PseI were more concentrated in groups II and III, respectively. We also found that PseI appeared later in the evolutionary process, derived from NeuB. We use this same methodology to retrieve sialic acid synthase sequences from Archaea and Eukarya. A large-scale phylogenetic analysis showed that while the Archaea sequences are spread across the tree, the eukaryotic NanS sequences were grouped in a specific branch in group II. None of the bacterial NanS sequences grouped with the eukaryotic branch. The analysis of conserved residues showed that the synthases of Archaea and Eukarya present a mutation in one of the three catalytic residues, an E134D change, related to a Neisseria meningitidis reference sequence. We also found that the conservation profile is higher between NeuB of pathogenic bacteria and NanS of eukaryotes than between NeuB of environmental bacteria and NanS of eukaryotes. Our large-scale analysis brings new perspectives on the evolution of NulOs synthases, suggesting their presence in the last common universal ancestor.MonosaccharidesN-Acetylneuraminic AcidBacterial InfectionsPhylogenyMonosacáridosÁcido N-AcetilneuramínicoInfecciones BacterianasFilogeniaOsesAcide N-acétyl-neuraminiqueInfections bactériennesPhylogenèseMonossacarídeosÁcido N-AcetilneuramínicoInfecções BacterianasFilogeniainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/47138/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALmgen000563ok.pdfmgen000563ok.pdfapplication/pdf7636184https://www.arca.fiocruz.br/bitstream/icict/47138/2/mgen000563ok.pdfd7226ecfe9121a62a2303bc3cbb4dc18MD52TEXTmgen000563ok.pdf.txtmgen000563ok.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis
title Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis
spellingShingle Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis
Vieira, Alexandre Zanatta
Monosaccharides
N-Acetylneuraminic Acid
Bacterial Infections
Phylogeny
Monosacáridos
Ácido N-Acetilneuramínico
Infecciones Bacterianas
Filogenia
Oses
Acide N-acétyl-neuraminique
Infections bactériennes
Phylogenèse
Monossacarídeos
Ácido N-Acetilneuramínico
Infecções Bacterianas
Filogenia
title_short Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis
title_full Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis
title_fullStr Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis
title_full_unstemmed Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis
title_sort Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis
author Vieira, Alexandre Zanatta
author_facet Vieira, Alexandre Zanatta
Raittz, Roberto Tadeu
Faoro, Helisson
author_role author
author2 Raittz, Roberto Tadeu
Faoro, Helisson
author2_role author
author
dc.contributor.author.fl_str_mv Vieira, Alexandre Zanatta
Raittz, Roberto Tadeu
Faoro, Helisson
dc.subject.en.pt_BR.fl_str_mv Monosaccharides
N-Acetylneuraminic Acid
Bacterial Infections
Phylogeny
topic Monosaccharides
N-Acetylneuraminic Acid
Bacterial Infections
Phylogeny
Monosacáridos
Ácido N-Acetilneuramínico
Infecciones Bacterianas
Filogenia
Oses
Acide N-acétyl-neuraminique
Infections bactériennes
Phylogenèse
Monossacarídeos
Ácido N-Acetilneuramínico
Infecções Bacterianas
Filogenia
dc.subject.es.pt_BR.fl_str_mv Monosacáridos
Ácido N-Acetilneuramínico
Infecciones Bacterianas
Filogenia
dc.subject.fr.pt_BR.fl_str_mv Oses
Acide N-acétyl-neuraminique
Infections bactériennes
Phylogenèse
dc.subject.decs.pt_BR.fl_str_mv Monossacarídeos
Ácido N-Acetilneuramínico
Infecções Bacterianas
Filogenia
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil / Universidade Federal do Paraná. Programa de Graduação em Bioinformática. Curitiba, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-05-11T19:34:01Z
dc.date.available.fl_str_mv 2021-05-11T19:34:01Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIEIRA, Alexandre Zanatta; RAITTZ, Roberto Tadeu; FAORO, Helisson. Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis. Microbial Genomics, v.7, n. 563, p. 1-14, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47138
dc.identifier.issn.pt_BR.fl_str_mv 2057-5858
dc.identifier.doi.none.fl_str_mv 10.1099/mgen.0.000563
identifier_str_mv VIEIRA, Alexandre Zanatta; RAITTZ, Roberto Tadeu; FAORO, Helisson. Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis. Microbial Genomics, v.7, n. 563, p. 1-14, 2021.
2057-5858
10.1099/mgen.0.000563
url https://www.arca.fiocruz.br/handle/icict/47138
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/47138/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/47138/2/mgen000563ok.pdf
https://www.arca.fiocruz.br/bitstream/icict/47138/3/mgen000563ok.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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