A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual

Detalhes bibliográficos
Autor(a) principal: Caccuri, Francesca
Data de Publicação: 2020
Outros Autores: Zanni, Alberto, Messali, Serena, Giovanetti, Marta, Bugatti, Antonella, Campisi, Giovanni, Filippini, Federica, Scaltriti, Erika, Ciccozzi, Massimo, Fiorentini, Simona, Caruso, Arnaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45901
Resumo: University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.
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spelling Caccuri, FrancescaZanni, AlbertoMessali, SerenaGiovanetti, MartaBugatti, AntonellaCampisi, GiovanniFilippini, FedericaScaltriti, ErikaCiccozzi, MassimoFiorentini, SimonaCaruso, Arnaldo2021-02-01T15:33:47Z2021-02-01T15:33:47Z2020CACCURI, Francesca et al. A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual. Journal of Translational Medicine, v. 18, n. 362, p. 1-12, 2020.1479-5876https://www.arca.fiocruz.br/handle/icict/4590110.1186/s12967-020-02535-1engBMCSARS-CoV-2Persistência do vírusInfecção assintomáticaIsolado do vírusVariação genéticaEpidemiaCOVID-19Virus persistenceSARS-CoV-2Asymptomatic infectionVirus isolateGenetic variationEpidemicCOVID-19A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individualinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.University Campus Bio-Medico of Rome. Unit of Medical Statistics and Molecular Epidemiology. Rome, Italy.University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.Background: Since the first outbreak of SARS-CoV-2, the clinical characteristics of the Coronavirus Disease 2019 (COVID-19) have been progressively changed. Data reporting a viral intra-host and inter-host evolution favouring the appearance of mild SARS-CoV-2 strains are since being accumulating. To better understand the evolution of SARSCoV- 2 pathogenicity and its adaptation to the host, it is therefore crucial to investigate the genetic and phenotypic characteristics of SARS-CoV-2 strains circulating lately in the epidemic. Methods: Nasopharyngeal swabs have been analyzed for viral load in the early (March 2020) and late (May 2020) phases of epidemic in Brescia, Italy. Isolation of SARS-CoV-2 from 2 high viral load specimens identified on March 9 (AP66) and on May 8 (GZ69) was performed on Vero E6 cells. Amount of virus released was assessed by quantitative PCR. Genotypic characterization of AP66 and GZ69 was performed by next generation sequencing followed by an in-depth in silico analysis of nucleotide mutations. Results: The SARS-CoV-2 GZ69 strain, isolated in May from an asymptomatic healthcare worker, showed an unprecedented capability of replication in Vero E6 cells in the absence of any evident cytopathic effect. Vero E6 subculturing, up to passage 4, showed that SARS-CoV-2 GZ69 infection was as productive as the one sustained by the cytopathic strain AP66. Whole genome sequencing of the persistently replicating SARS-CoV-2 GZ69 has shown that this strain differs from the early AP66 variant in 9 nucleotide positions (C2939T; C3828T; G21784T; T21846C; T24631C; G28881A; G28882A; G28883C; G29810T) which lead to 6 non-synonymous substitutions spanning on ORF1ab (P892S; S1188L), S (K74N; I95T) and N (R203K, G204R) proteins. Conclusions: Identification of the peculiar SARS-CoV-2 GZ69 strain in the late Italian epidemic highlights the need to better characterize viral variants circulating among asymptomatic or paucisymptomatic individuals. The current approach could unravel the ways for future studies aimed at analyzing the selection process which favours viral mutations in the human host.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45901/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMartaGIovanetti_MassmoCiccozi_etal_IOC_2020.pdfMartaGIovanetti_MassmoCiccozi_etal_IOC_2020.pdfapplication/pdf3132803https://www.arca.fiocruz.br/bitstream/icict/45901/2/MartaGIovanetti_MassmoCiccozi_etal_IOC_2020.pdfabc665b7ba7a245045d01ea2bfde9ad9MD52TEXTMartaGIovanetti_MassmoCiccozi_etal_IOC_2020.pdf.txtMartaGIovanetti_MassmoCiccozi_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual
title A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual
spellingShingle A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual
Caccuri, Francesca
SARS-CoV-2
Persistência do vírus
Infecção assintomática
Isolado do vírus
Variação genética
Epidemia
COVID-19
Virus persistence
SARS-CoV-2
Asymptomatic infection
Virus isolate
Genetic variation
Epidemic
COVID-19
title_short A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual
title_full A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual
title_fullStr A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual
title_full_unstemmed A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual
title_sort A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual
author Caccuri, Francesca
author_facet Caccuri, Francesca
Zanni, Alberto
Messali, Serena
Giovanetti, Marta
Bugatti, Antonella
Campisi, Giovanni
Filippini, Federica
Scaltriti, Erika
Ciccozzi, Massimo
Fiorentini, Simona
Caruso, Arnaldo
author_role author
author2 Zanni, Alberto
Messali, Serena
Giovanetti, Marta
Bugatti, Antonella
Campisi, Giovanni
Filippini, Federica
Scaltriti, Erika
Ciccozzi, Massimo
Fiorentini, Simona
Caruso, Arnaldo
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Caccuri, Francesca
Zanni, Alberto
Messali, Serena
Giovanetti, Marta
Bugatti, Antonella
Campisi, Giovanni
Filippini, Federica
Scaltriti, Erika
Ciccozzi, Massimo
Fiorentini, Simona
Caruso, Arnaldo
dc.subject.other.pt_BR.fl_str_mv SARS-CoV-2
Persistência do vírus
Infecção assintomática
Isolado do vírus
Variação genética
Epidemia
COVID-19
topic SARS-CoV-2
Persistência do vírus
Infecção assintomática
Isolado do vírus
Variação genética
Epidemia
COVID-19
Virus persistence
SARS-CoV-2
Asymptomatic infection
Virus isolate
Genetic variation
Epidemic
COVID-19
dc.subject.en.pt_BR.fl_str_mv Virus persistence
SARS-CoV-2
Asymptomatic infection
Virus isolate
Genetic variation
Epidemic
COVID-19
description University of Brescia. Department of Molecular and Translational Medicine. Section of Microbiology. Brescia, Italy.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-02-01T15:33:47Z
dc.date.available.fl_str_mv 2021-02-01T15:33:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CACCURI, Francesca et al. A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual. Journal of Translational Medicine, v. 18, n. 362, p. 1-12, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45901
dc.identifier.issn.pt_BR.fl_str_mv 1479-5876
dc.identifier.doi.none.fl_str_mv 10.1186/s12967-020-02535-1
identifier_str_mv CACCURI, Francesca et al. A persistently replicating SARS‑CoV‑2 variant derived from an asymptomatic individual. Journal of Translational Medicine, v. 18, n. 362, p. 1-12, 2020.
1479-5876
10.1186/s12967-020-02535-1
url https://www.arca.fiocruz.br/handle/icict/45901
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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