Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp

Detalhes bibliográficos
Autor(a) principal: Araujo, Paula Finamore
Data de Publicação: 2021
Outros Autores: Pereira, Amanda Faler, Brito, Carlos Ramon do Nascimento, Peres, Eldrinei Gomes, Yamaguchi, Klenicy Kazumy de Lima, Ferreira, Renata Trotta Barroso, Moreira, Octacilio Cruz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/48247
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Plataforma Fiocruz de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil.
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spelling Araujo, Paula FinamorePereira, Amanda FalerBrito, Carlos Ramon do NascimentoPeres, Eldrinei GomesYamaguchi, Klenicy Kazumy de LimaFerreira, Renata Trotta BarrosoMoreira, Octacilio Cruz2021-07-15T13:38:54Z2021-07-15T13:38:54Z2021ARAUJO, Paula Finamore et al. Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp. Plos One, v. 16, n. 2, e0246435, 17 p, Feb. 2021.1932-6203https://www.arca.fiocruz.br/handle/icict/4824710.1371/journal.pone.0246435engPublic Library of ScienceValidaçãoNovo ensaio PCR multiplexTempo realDetecçãoQuantificaçãoTrypanosoma cruziPolpa de açaiValidationNovel multiplex real-time PCR assayTrypanosoma cruzi detectionQuantificationAçai pulpValidation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulpinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Plataforma Fiocruz de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Plataforma Fiocruz de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Departamento de Anáises Clínicas e Toxicológicas. Natal, RN, Brasil.Universidade Federal do Amazonas. Departamento de Quimica. Manaus, AM, Brasil.Universidade Federal do Amazonas. Instituto de Saúde e Biotecnologia. Campus Coari, AM, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Plataforma Fiocruz de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil.In Brazil, orally acquired T. cruzi infection has become the most relevant transmission mech anisms from public health perspective. Around 70% of new Chagas disease cases have been associated with consumption of contaminated food or beverages. Ac¸ai (Euterpe olera cea and Euterpe precatoria) is currently one of the most commercialized Amazonian fruits in the Brazilian and international markets. Therefore, it has become important to incorporate in the production process some procedures to measure out effective hygiene and product quality control required by global market. Molecular methods have been developed for rapid detection and quantification of T. cruzi DNA in several biological samples, including food matrices, for epidemiological investigation of Chagas disease and food quality control. How ever, a high-performance molecular methodology since DNA extraction until detection and quantification of T. cruzi DNA in ac¸ai berry pulp is still needed. Herein, a simple DNA extrac tion methodology was standardized from the supernatant of ac¸ai berry pulp stabilized in a 6M Guanidine-HCl/0.2M EDTA buffer. In addition, a multiplex real time qPCR assay, target ing T. cruzi DNA and an Exogenous Internal Positive Control was developed and validated, using reference from all T. cruzi DTUs and commercial samples of ac¸ai pulp, from an endemic municipality with previous history of oral Chagas disease outbreak. Thus, a high sensitivity qPCR assay, that could detect up to 0.01 parasite equivalents/mL in ac¸ai, was reached. As of the 45 commercial samples analyzed, 9 (20%) were positive for T. cruzi. This high-sensitive, fast, and easy-to-use molecular assay is compatible with most of the labora tories involved in the investigations of oral Chagas disease outbreaks, representing an important tool to the epidemiology, control, and surveillance of Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48247/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAmandaF_Pereira_etl_IOC_2021.pdfAmandaF_Pereira_etl_IOC_2021.pdfapplication/pdf1039553https://www.arca.fiocruz.br/bitstream/icict/48247/2/AmandaF_Pereira_etl_IOC_2021.pdf591da1811817ce393e7c2b73ebee559aMD52TEXTAmandaF_Pereira_etl_IOC_2021.pdf.txtAmandaF_Pereira_etl_IOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp
title Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp
spellingShingle Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp
Araujo, Paula Finamore
Validação
Novo ensaio PCR multiplex
Tempo real
Detecção
Quantificação
Trypanosoma cruzi
Polpa de açai
Validation
Novel multiplex real-time PCR assay
Trypanosoma cruzi detection
Quantification
Açai pulp
title_short Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp
title_full Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp
title_fullStr Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp
title_full_unstemmed Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp
title_sort Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp
author Araujo, Paula Finamore
author_facet Araujo, Paula Finamore
Pereira, Amanda Faler
Brito, Carlos Ramon do Nascimento
Peres, Eldrinei Gomes
Yamaguchi, Klenicy Kazumy de Lima
Ferreira, Renata Trotta Barroso
Moreira, Octacilio Cruz
author_role author
author2 Pereira, Amanda Faler
Brito, Carlos Ramon do Nascimento
Peres, Eldrinei Gomes
Yamaguchi, Klenicy Kazumy de Lima
Ferreira, Renata Trotta Barroso
Moreira, Octacilio Cruz
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo, Paula Finamore
Pereira, Amanda Faler
Brito, Carlos Ramon do Nascimento
Peres, Eldrinei Gomes
Yamaguchi, Klenicy Kazumy de Lima
Ferreira, Renata Trotta Barroso
Moreira, Octacilio Cruz
dc.subject.other.pt_BR.fl_str_mv Validação
Novo ensaio PCR multiplex
Tempo real
Detecção
Quantificação
Trypanosoma cruzi
Polpa de açai
topic Validação
Novo ensaio PCR multiplex
Tempo real
Detecção
Quantificação
Trypanosoma cruzi
Polpa de açai
Validation
Novel multiplex real-time PCR assay
Trypanosoma cruzi detection
Quantification
Açai pulp
dc.subject.en.pt_BR.fl_str_mv Validation
Novel multiplex real-time PCR assay
Trypanosoma cruzi detection
Quantification
Açai pulp
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Plataforma Fiocruz de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-07-15T13:38:54Z
dc.date.available.fl_str_mv 2021-07-15T13:38:54Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAUJO, Paula Finamore et al. Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp. Plos One, v. 16, n. 2, e0246435, 17 p, Feb. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/48247
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0246435
identifier_str_mv ARAUJO, Paula Finamore et al. Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp. Plos One, v. 16, n. 2, e0246435, 17 p, Feb. 2021.
1932-6203
10.1371/journal.pone.0246435
url https://www.arca.fiocruz.br/handle/icict/48247
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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