Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections

Detalhes bibliográficos
Autor(a) principal: Malta, Fábio Correia
Data de Publicação: 2020
Outros Autores: Varella, Rafael Brandão, Guimarães, Maria Angelica Arpon Marandino, Miagostovich, Marize Pereira, Fumian, Tulio Machado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45714
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Escola de Medicina. Departamento de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.
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spelling Malta, Fábio CorreiaVarella, Rafael BrandãoGuimarães, Maria Angelica Arpon MarandinoMiagostovich, Marize PereiraFumian, Tulio Machado2021-01-15T13:54:39Z2021-01-15T13:54:39Z2020MALTA, Fábio Correia et al. Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections. Pathogens, v. 9, n. 645, p. 1-14, 2020.2076-0817https://www.arca.fiocruz.br/handle/icict/4571410.3390/pathogens9080645engMDPIGastroenterite agudaBocavírus humanoCo-infecçõesCarga viralGenotipagemBrasilAcute gastroenteritisHuman bocavirusCo-infectionsViral loadGenotypingBrazilHuman Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Escola de Medicina. Departamento de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia.. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Escola de Medicina. Departamento de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Human bocavirus (HBoV) is an emerging virus and has been detected worldwide, especially in pediatric patients with respiratory and gastrointestinal infection. In this study, we describe HBoV prevalence, genotypes circulation andDNAshedding, in stool samples fromchildren up to two years of age in Brazil. During 2016 and 2017, 886 acute gastroenteritis (AGE) stool samples from ten Brazilian states were analyzed by TaqMan®-based qPCR, to detect and quantify HBoV. Positive samples were genotyped by sequencing the VP1/2 overlap region, followed by phylogenetic analysis and co-infections were accessed by screening other gastroenteric viruses. HBoV was detected in 12.4% (n = 110) of samples, with viral load ranging from 1.6 102 to 1.2 109 genome copies per gram of stool. From these, co-infections were found in 79.1%, and a statistically lower HBoV viral load was found compared to viral loads of rotavirus, norovirus and adenovirus in double infected patients (p < 0.05). No significant di erences were found between HBoV viral load in single or co-infections, age groups or genotypes. Phylogenetic analysis identified the circulation of HBoV-1 in 38%, HBoV-2 in 40% and HBoV-3 in 22%. Continuous HBoV monitoring is needed to clarify its role in diarrhea disease, especially in the absence of classic gastroenteric viruses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45714/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFabioMalta_TulioFumian_etal_IOC_2020.pdfFabioMalta_TulioFumian_etal_IOC_2020.pdfapplication/pdf1799668https://www.arca.fiocruz.br/bitstream/icict/45714/2/FabioMalta_TulioFumian_etal_IOC_2020.pdf8c008c8734502a15ed41aff79c2cb048MD52TEXTFabioMalta_TulioFumian_etal_IOC_2020.pdf.txtFabioMalta_TulioFumian_etal_IOC_2020.pdf.txtExtracted texttext/plain55682https://www.arca.fiocruz.br/bitstream/icict/45714/3/FabioMalta_TulioFumian_etal_IOC_2020.pdf.txt451e287662b215c4fa3df0e659a403bcMD53icict/457142022-06-24 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dc.title.pt_BR.fl_str_mv Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
title Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
spellingShingle Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
Malta, Fábio Correia
Gastroenterite aguda
Bocavírus humano
Co-infecções
Carga viral
Genotipagem
Brasil
Acute gastroenteritis
Human bocavirus
Co-infections
Viral load
Genotyping
Brazil
title_short Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
title_full Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
title_fullStr Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
title_full_unstemmed Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
title_sort Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections
author Malta, Fábio Correia
author_facet Malta, Fábio Correia
Varella, Rafael Brandão
Guimarães, Maria Angelica Arpon Marandino
Miagostovich, Marize Pereira
Fumian, Tulio Machado
author_role author
author2 Varella, Rafael Brandão
Guimarães, Maria Angelica Arpon Marandino
Miagostovich, Marize Pereira
Fumian, Tulio Machado
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Malta, Fábio Correia
Varella, Rafael Brandão
Guimarães, Maria Angelica Arpon Marandino
Miagostovich, Marize Pereira
Fumian, Tulio Machado
dc.subject.other.pt_BR.fl_str_mv Gastroenterite aguda
Bocavírus humano
Co-infecções
Carga viral
Genotipagem
Brasil
topic Gastroenterite aguda
Bocavírus humano
Co-infecções
Carga viral
Genotipagem
Brasil
Acute gastroenteritis
Human bocavirus
Co-infections
Viral load
Genotyping
Brazil
dc.subject.en.pt_BR.fl_str_mv Acute gastroenteritis
Human bocavirus
Co-infections
Viral load
Genotyping
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Escola de Medicina. Departamento de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-15T13:54:39Z
dc.date.available.fl_str_mv 2021-01-15T13:54:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MALTA, Fábio Correia et al. Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections. Pathogens, v. 9, n. 645, p. 1-14, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45714
dc.identifier.issn.pt_BR.fl_str_mv 2076-0817
dc.identifier.doi.none.fl_str_mv 10.3390/pathogens9080645
identifier_str_mv MALTA, Fábio Correia et al. Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections. Pathogens, v. 9, n. 645, p. 1-14, 2020.
2076-0817
10.3390/pathogens9080645
url https://www.arca.fiocruz.br/handle/icict/45714
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/45714/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/45714/2/FabioMalta_TulioFumian_etal_IOC_2020.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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