Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates

Detalhes bibliográficos
Autor(a) principal: Vilardo, Mônica de Castro Britto
Data de Publicação: 2006
Outros Autores: Thomé, Jacqueline Darc da Silva, Esteves, Wagner Thadeu Cardoso, Filgueiras, Ana Luzia Lauria, Oliveira, Selma Soares de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39762
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
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spelling Vilardo, Mônica de Castro BrittoThomé, Jacqueline Darc da SilvaEsteves, Wagner Thadeu CardosoFilgueiras, Ana Luzia LauriaOliveira, Selma Soares de2020-02-06T12:18:06Z2020-02-06T12:18:06Z2006VILARDO, Mônica de Castro Brito et al. Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 5, p. 499-501, Aug. 2006.0074-0206https://www.arca.fiocruz.br/handle/icict/3976210.1590/s0074-027620060005000031678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzCampylobacterIdentificaçãoPrimatas não-humanosCampylobacterIdentificationNon-human primatesApplication of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Genética de Bactérias Associadas a Alimentos. Rio de Janeiro, RJ, Brasil.A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39762/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMOnicaBVillardo_JaquelineThome_etal_IOC_2006.pdfMOnicaBVillardo_JaquelineThome_etal_IOC_2006.pdfapplication/pdf436119https://www.arca.fiocruz.br/bitstream/icict/39762/2/MOnicaBVillardo_JaquelineThome_etal_IOC_2006.pdfcf0335a5a0b6c3cfe690c7522601d764MD52TEXTMOnicaBVillardo_JaquelineThome_etal_IOC_2006.pdf.txtMOnicaBVillardo_JaquelineThome_etal_IOC_2006.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
spellingShingle Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
Vilardo, Mônica de Castro Britto
Campylobacter
Identificação
Primatas não-humanos
Campylobacter
Identification
Non-human primates
title_short Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title_full Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title_fullStr Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title_full_unstemmed Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title_sort Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
author Vilardo, Mônica de Castro Britto
author_facet Vilardo, Mônica de Castro Britto
Thomé, Jacqueline Darc da Silva
Esteves, Wagner Thadeu Cardoso
Filgueiras, Ana Luzia Lauria
Oliveira, Selma Soares de
author_role author
author2 Thomé, Jacqueline Darc da Silva
Esteves, Wagner Thadeu Cardoso
Filgueiras, Ana Luzia Lauria
Oliveira, Selma Soares de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vilardo, Mônica de Castro Britto
Thomé, Jacqueline Darc da Silva
Esteves, Wagner Thadeu Cardoso
Filgueiras, Ana Luzia Lauria
Oliveira, Selma Soares de
dc.subject.other.pt_BR.fl_str_mv Campylobacter
Identificação
Primatas não-humanos
topic Campylobacter
Identificação
Primatas não-humanos
Campylobacter
Identification
Non-human primates
dc.subject.en.pt_BR.fl_str_mv Campylobacter
Identification
Non-human primates
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2020-02-06T12:18:06Z
dc.date.available.fl_str_mv 2020-02-06T12:18:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VILARDO, Mônica de Castro Brito et al. Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 5, p. 499-501, Aug. 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39762
dc.identifier.issn.pt_BR.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762006000500003
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv VILARDO, Mônica de Castro Brito et al. Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 5, p. 499-501, Aug. 2006.
0074-0206
10.1590/s0074-02762006000500003
1678-8060
url https://www.arca.fiocruz.br/handle/icict/39762
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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