LM14 defined medium enables continuous growth of Trypanosoma cruzi
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8871 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. |
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Lima, Carla Vanessa de PaulaBatista, MichelKugeratski, Fernanda G.Vincent, Isabel M.Soares, Maurilio JoséProbst, Christian MacagnanKrieger, Marco AurélioMarchini, Fabrício Klerynton2014-11-14T15:04:51Z2014-11-14T15:04:51Z2014LIMA, Carla V. de Paula et al. LM14 defined medium enables continuous growth of Trypanosoma cruzi. BMC Microbiology, v.14, n. 238, p. 1-8, 2014.1471-2180https://www.arca.fiocruz.br/handle/icict/887110.1186/s12866-014-0238-yengBiomed CentralLM14 defined medium enables continuous growth of Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.University of Glasgow. College of Medical, Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. The Wellcome Trust Centre for Molecular Parasitology. Glasgow, UK.Background Trypanosoma cruzi, the etiologic agent of Chagas disease, alternates between distinct morphological and functional forms during its life cycle. Axenic multiplication and differentiation processes of this protozoan parasite can be reproduced in vitro, enabling the isolation and study of the different evolutionary forms. Although there are several publications attempting the cultivation of T. cruzi under chemically defined conditions, in our experience none of the published media are capable of maintaining T. cruzi in continuous growth. Results In this work we modified a known chemically defined medium for Trypanosoma brucei growth. The resulting LM14 and LM14B defined media enabled cultivation of five different strains of T. cruzi for more than forty passages until now. The parasite’s biological characteristics such as morphology and differentiation to metacyclic trypomastigotes were maintained when defined media is used. Conclusions The establishment of a defined medium for T. cruzi cultivation is an important tool for basic biological research allowing several different approaches, providing new perspectives for further studies related to cell biology of this parasite.Trypanosoma cruziChemically defined mediumLife CycleCiclo de VidaParasitosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8871/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_Lima_etal_LM14 defined medium enables continuous.pdf2014_Lima_etal_LM14 defined medium enables continuous.pdfdocumento principalapplication/pdf1269991https://www.arca.fiocruz.br/bitstream/icict/8871/2/2014_Lima_etal_LM14%20defined%20medium%20enables%20continuous.pdf570520ef84561415d6984f6dd07298a5MD52LM14B preparation method.xlsxLM14B preparation method.xlsxapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheet17970https://www.arca.fiocruz.br/bitstream/icict/8871/3/LM14B%20preparation%20method.xlsxd4eb8fe20cd7a69207d5d47d45706a18MD53Ultrastructural morphology of T. cruzi epimastigotes under cultivation in different culture media.docUltrastructural morphology of T. cruzi epimastigotes under cultivation in different culture media.docapplication/msword6547456https://www.arca.fiocruz.br/bitstream/icict/8871/4/Ultrastructural%20morphology%20of%20T.%20cruzi%20epimastigotes%20under%20cultivation%20in%20different%20culture%20media.docfcf0f013c285225981ee39b46df93f62MD54TEXTUltrastructural morphology of T. cruzi epimastigotes under cultivation in different culture media.doc.txtUltrastructural morphology of T. cruzi epimastigotes under cultivation in different culture media.doc.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
LM14 defined medium enables continuous growth of Trypanosoma cruzi |
| title |
LM14 defined medium enables continuous growth of Trypanosoma cruzi |
| spellingShingle |
LM14 defined medium enables continuous growth of Trypanosoma cruzi Lima, Carla Vanessa de Paula Trypanosoma cruzi Chemically defined medium Life Cycle Ciclo de Vida Parasitos |
| title_short |
LM14 defined medium enables continuous growth of Trypanosoma cruzi |
| title_full |
LM14 defined medium enables continuous growth of Trypanosoma cruzi |
| title_fullStr |
LM14 defined medium enables continuous growth of Trypanosoma cruzi |
| title_full_unstemmed |
LM14 defined medium enables continuous growth of Trypanosoma cruzi |
| title_sort |
LM14 defined medium enables continuous growth of Trypanosoma cruzi |
| author |
Lima, Carla Vanessa de Paula |
| author_facet |
Lima, Carla Vanessa de Paula Batista, Michel Kugeratski, Fernanda G. Vincent, Isabel M. Soares, Maurilio José Probst, Christian Macagnan Krieger, Marco Aurélio Marchini, Fabrício Klerynton |
| author_role |
author |
| author2 |
Batista, Michel Kugeratski, Fernanda G. Vincent, Isabel M. Soares, Maurilio José Probst, Christian Macagnan Krieger, Marco Aurélio Marchini, Fabrício Klerynton |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Carla Vanessa de Paula Batista, Michel Kugeratski, Fernanda G. Vincent, Isabel M. Soares, Maurilio José Probst, Christian Macagnan Krieger, Marco Aurélio Marchini, Fabrício Klerynton |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Chemically defined medium Life Cycle |
| topic |
Trypanosoma cruzi Chemically defined medium Life Cycle Ciclo de Vida Parasitos |
| dc.subject.decs.pt_BR.fl_str_mv |
Ciclo de Vida Parasitos |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. |
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2014 |
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2014-11-14T15:04:51Z |
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2014-11-14T15:04:51Z |
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2014 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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LIMA, Carla V. de Paula et al. LM14 defined medium enables continuous growth of Trypanosoma cruzi. BMC Microbiology, v.14, n. 238, p. 1-8, 2014. |
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https://www.arca.fiocruz.br/handle/icict/8871 |
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1471-2180 |
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10.1186/s12866-014-0238-y |
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LIMA, Carla V. de Paula et al. LM14 defined medium enables continuous growth of Trypanosoma cruzi. BMC Microbiology, v.14, n. 238, p. 1-8, 2014. 1471-2180 10.1186/s12866-014-0238-y |
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Biomed Central |
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Biomed Central |
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