Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays

Detalhes bibliográficos
Autor(a) principal: Castro Junior, José Geraldo
Data de Publicação: 2014
Outros Autores: Freire, Mariana Lourenço, Campos, Samantha Priscila Silva, Scopel, Kezia K. G, Porrozzi, Renato, Silva, Edmilson Domingos da, Colombo, Fábio A, Silveira, Rita de Cássia Viveiros da, Marques, Marcos José, Coimbra, Elaine Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10699
Resumo: Universidade Federal de Juiz de Fora. Programa de Pós-graduação em Saúde Brasileira. Juiz de Fora, MG, Brasil / Secretaria Municipal de Saúde de Juiz de Fora. Departamento de Vigilância Epidemiológica e Ambiental. Setor de Zoonoses. Juiz de Fora, MG, Brasil.
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spelling Castro Junior, José GeraldoFreire, Mariana LourençoCampos, Samantha Priscila SilvaScopel, Kezia K. GPorrozzi, RenatoSilva, Edmilson Domingos daColombo, Fábio ASilveira, Rita de Cássia Viveiros daMarques, Marcos JoséCoimbra, Elaine Soares2015-06-08T14:01:52Z2015-06-08T14:01:52Z2014CASTRO JUNIOR, José Geraldo et al. Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays. Rev. Inst. Med. Trop. Sao Paulo, v.56, n.3, p.225-229, May-June, 2014.0036-4665https://www.arca.fiocruz.br/handle/icict/1069910.1590/S0036-46652014000300008engInstituto de Medicina Tropical de São PauloEvidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assaysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Juiz de Fora. Programa de Pós-graduação em Saúde Brasileira. Juiz de Fora, MG, Brasil / Secretaria Municipal de Saúde de Juiz de Fora. Departamento de Vigilância Epidemiológica e Ambiental. Setor de Zoonoses. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Intituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Intituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Biomanguinhos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Departamento de Patologia e Parasitologia. Laboratório de Parasitologia. Alfenas, MG, Brasil.Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Departamento de Patologia e Parasitologia. Laboratório de Parasitologia. Alfenas, MG, Brasil.Universidade Federal de Alfenas. Instituto de Ciências Biomédicas. Departamento de Patologia e Parasitologia. Laboratório de Parasitologia. Alfenas, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.In Brazil, domestic dogs are branded as the primary reservoir for zoonotic visceral leishmaniasis, due to the clear positive correlation observed between human and canine infection rates. This study aimed to carry out a serological survey of canine visceral leishmaniasis (CVL) in dogs housed at a public kennel in the municipality of Juiz de Fora, Minas Gerais State, Brazil, using the immunochromatographic TR DPP® CVL rapid test. Additionally, conventional and/or real time PCR assay was used to detect and confirm L. infantum infection in the DPP positive dogs only. Of the 400 dogs studied, most did not present clinical signs for CVL (p < 0.05), and fifteen (3.8%) were seropositive in the DPP test. There was no statistically significant difference between the DPP seropositive dogs and the clinical signs of the disease (p > 0.05). Both conventional and real time PCR tests confirmed L. infantum infection in nine (75.0%) of the twelve DPP seropositive dogs that remained alive during the follow-up period. This study is the first seroepidemiologic survey of CVL held in the city of Juiz de Fora, and the results reinforce the idea that this disease is currently in a process of expansion and urbanization in Brazil. Furthermore, this study highlights the use of the DPP test as an alternative for diagnosing CVL in large and mid-sized cities, due to its ease of implementation.Canine visceral leishmaniasisSerological diagnosisImmunochromatographic diagnosisDPPPCR diagnosisLeishmaniose VisceralCãesDiagnósticoReação em Cadeia da Polimeraseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10699/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALrenato_porrozzietal_IOC_2014.pdfapplication/pdf431937https://www.arca.fiocruz.br/bitstream/icict/10699/2/renato_porrozzietal_IOC_2014.pdf936bcf6e69d7c33d9b39d44ce0e75a8aMD52TEXTrenato_porrozzietal_IOC_2014.pdf.txtrenato_porrozzietal_IOC_2014.pdf.txtExtracted texttext/plain27641https://www.arca.fiocruz.br/bitstream/icict/10699/3/renato_porrozzietal_IOC_2014.pdf.txt0dabe08ba00a01fec5019821c3c4553dMD53icict/106992022-06-24 12:17:45.97oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:45Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
title Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
spellingShingle Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
Castro Junior, José Geraldo
Canine visceral leishmaniasis
Serological diagnosis
Immunochromatographic diagnosis
DPP
PCR diagnosis
Leishmaniose Visceral
Cães
Diagnóstico
Reação em Cadeia da Polimerase
title_short Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
title_full Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
title_fullStr Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
title_full_unstemmed Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
title_sort Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
author Castro Junior, José Geraldo
author_facet Castro Junior, José Geraldo
Freire, Mariana Lourenço
Campos, Samantha Priscila Silva
Scopel, Kezia K. G
Porrozzi, Renato
Silva, Edmilson Domingos da
Colombo, Fábio A
Silveira, Rita de Cássia Viveiros da
Marques, Marcos José
Coimbra, Elaine Soares
author_role author
author2 Freire, Mariana Lourenço
Campos, Samantha Priscila Silva
Scopel, Kezia K. G
Porrozzi, Renato
Silva, Edmilson Domingos da
Colombo, Fábio A
Silveira, Rita de Cássia Viveiros da
Marques, Marcos José
Coimbra, Elaine Soares
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Castro Junior, José Geraldo
Freire, Mariana Lourenço
Campos, Samantha Priscila Silva
Scopel, Kezia K. G
Porrozzi, Renato
Silva, Edmilson Domingos da
Colombo, Fábio A
Silveira, Rita de Cássia Viveiros da
Marques, Marcos José
Coimbra, Elaine Soares
dc.subject.en.pt_BR.fl_str_mv Canine visceral leishmaniasis
Serological diagnosis
Immunochromatographic diagnosis
DPP
PCR diagnosis
topic Canine visceral leishmaniasis
Serological diagnosis
Immunochromatographic diagnosis
DPP
PCR diagnosis
Leishmaniose Visceral
Cães
Diagnóstico
Reação em Cadeia da Polimerase
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose Visceral
Cães
Diagnóstico
Reação em Cadeia da Polimerase
description Universidade Federal de Juiz de Fora. Programa de Pós-graduação em Saúde Brasileira. Juiz de Fora, MG, Brasil / Secretaria Municipal de Saúde de Juiz de Fora. Departamento de Vigilância Epidemiológica e Ambiental. Setor de Zoonoses. Juiz de Fora, MG, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:52Z
dc.date.available.fl_str_mv 2015-06-08T14:01:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CASTRO JUNIOR, José Geraldo et al. Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays. Rev. Inst. Med. Trop. Sao Paulo, v.56, n.3, p.225-229, May-June, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10699
dc.identifier.issn.pt_BR.fl_str_mv 0036-4665
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S0036-46652014000300008
identifier_str_mv CASTRO JUNIOR, José Geraldo et al. Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays. Rev. Inst. Med. Trop. Sao Paulo, v.56, n.3, p.225-229, May-June, 2014.
0036-4665
10.1590/S0036-46652014000300008
url https://www.arca.fiocruz.br/handle/icict/10699
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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